Aquicella lusitana

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Aquicella

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2370 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370G8C2|A0A370G8C2_9COXI Hemolysin activation/secretion protein OS=Aquicella lusitana OX=254246 GN=C8D86_1242 PE=3 SV=1
MM1 pKa = 7.88GIGNIATTGMQAAMTNMEE19 pKa = 4.59VISNNIANSNTFGYY33 pKa = 9.52KK34 pKa = 10.12GSYY37 pKa = 11.04ANFADD42 pKa = 4.74MYY44 pKa = 9.92PSGSNASSVQAGLGVSVSGIQQDD67 pKa = 4.22FSPGGPTPTGIASDD81 pKa = 3.68LTITNDD87 pKa = 3.05GFFVLKK93 pKa = 10.33DD94 pKa = 3.47ASSGQTSYY102 pKa = 11.65SRR104 pKa = 11.84FGRR107 pKa = 11.84FNFNQGYY114 pKa = 9.81FMVGNQRR121 pKa = 11.84LQGFPAVNNTIPPGSTPTDD140 pKa = 3.82LYY142 pKa = 10.69INTASIPAQATGTITQQQLNLNASDD167 pKa = 4.38SVPSTTPFDD176 pKa = 4.88PNDD179 pKa = 3.5PTSFNFSSTATVYY192 pKa = 11.09DD193 pKa = 3.73SLGSSNTLNLYY204 pKa = 8.13YY205 pKa = 10.83VKK207 pKa = 9.78TAANEE212 pKa = 4.12WTVNAYY218 pKa = 10.71VNGTSVGTGSLTFSSDD234 pKa = 2.95GTLSSATGLSNLSFSPTSGATSPQVFSVSMTGATQYY270 pKa = 11.47ANPYY274 pKa = 6.78TANPFSSDD282 pKa = 2.78GYY284 pKa = 10.24AAGNFTGYY292 pKa = 9.28TIDD295 pKa = 3.55KK296 pKa = 9.38NGKK299 pKa = 7.45VTVNYY304 pKa = 10.55SNNQTTVAGQIALAMFQSPQGLQNVGNMSWIATSDD339 pKa = 3.38SGEE342 pKa = 4.2PNVNQANSQNGIQQAALEE360 pKa = 4.18MSNVDD365 pKa = 3.93LASEE369 pKa = 4.29MVSLITAQNIFQANAQVEE387 pKa = 4.29QVYY390 pKa = 9.31NQVMQTVTKK399 pKa = 10.48LAA401 pKa = 3.85

Molecular weight: 41.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370GMY4|A0A370GMY4_9COXI Uncharacterized protein OS=Aquicella lusitana OX=254246 GN=C8D86_11054 PE=4 SV=1
MM1 pKa = 7.52AKK3 pKa = 10.04ARR5 pKa = 11.84SSAKK9 pKa = 10.22RR10 pKa = 11.84KK11 pKa = 6.51TSKK14 pKa = 9.85VAKK17 pKa = 9.79AKK19 pKa = 9.53PRR21 pKa = 11.84KK22 pKa = 6.55TARR25 pKa = 11.84KK26 pKa = 8.9KK27 pKa = 9.42KK28 pKa = 7.08TAKK31 pKa = 8.89TVKK34 pKa = 10.14KK35 pKa = 10.15AATKK39 pKa = 10.38KK40 pKa = 8.92RR41 pKa = 11.84TGGRR45 pKa = 11.84KK46 pKa = 8.02RR47 pKa = 11.84TTAKK51 pKa = 10.02RR52 pKa = 11.84KK53 pKa = 9.3KK54 pKa = 9.5AAKK57 pKa = 9.68KK58 pKa = 10.12

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2370

0

2370

748558

25

2565

315.8

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.487 ± 0.051

1.084 ± 0.015

4.787 ± 0.039

5.875 ± 0.057

4.395 ± 0.043

6.259 ± 0.049

2.519 ± 0.029

7.336 ± 0.043

6.065 ± 0.058

10.705 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.024

4.272 ± 0.047

4.329 ± 0.034

4.386 ± 0.036

4.837 ± 0.042

6.105 ± 0.044

5.323 ± 0.047

6.151 ± 0.039

1.168 ± 0.022

3.383 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski