Xenorhabdus doucetiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Morganellaceae; Xenorhabdus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3749 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068QVP2|A0A068QVP2_9GAMM Glycine cleavage system transcriptional activator OS=Xenorhabdus doucetiae OX=351671 GN=gcvA PE=3 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.69DD4 pKa = 4.1AALPLQFTDD13 pKa = 3.4AAANKK18 pKa = 9.7VKK20 pKa = 10.87VLVADD25 pKa = 3.8EE26 pKa = 5.16DD27 pKa = 4.14NPNLRR32 pKa = 11.84LRR34 pKa = 11.84VYY36 pKa = 9.12ITGGGCSGFQYY47 pKa = 11.03GFTFDD52 pKa = 4.96DD53 pKa = 4.21QINDD57 pKa = 3.28GDD59 pKa = 3.99MTIQKK64 pKa = 10.01QGVEE68 pKa = 4.12LVVDD72 pKa = 4.31PMSLQYY78 pKa = 11.18LVGGCVDD85 pKa = 3.48YY86 pKa = 11.55TEE88 pKa = 4.72GLEE91 pKa = 4.02GSRR94 pKa = 11.84FIVTNPNAKK103 pKa = 6.23TTCGCGSSFSII114 pKa = 5.02

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068QXF7|A0A068QXF7_9GAMM Maltose/maltodextrin import ATP-binding protein MalK OS=Xenorhabdus doucetiae OX=351671 GN=malK PE=3 SV=1
MM1 pKa = 7.11SQYY4 pKa = 10.51HH5 pKa = 5.8SQGLWVIWLSFLIAIVLQIMPWPEE29 pKa = 3.63QFFMFRR35 pKa = 11.84PTLLMLCLIYY45 pKa = 10.15WLLALPHH52 pKa = 6.34RR53 pKa = 11.84VSVGTGFVLGMITDD67 pKa = 4.88LLHH70 pKa = 6.86GSILGAHH77 pKa = 7.17ALAFSLISFLVAFKK91 pKa = 10.5FQLIRR96 pKa = 11.84NMALWQQALVVVGLSTLMNLCVFLVHH122 pKa = 7.39ALLLKK127 pKa = 10.36PLFHH131 pKa = 7.66PEE133 pKa = 4.02VFWNGVVNGILWPWLFLLMRR153 pKa = 11.84KK154 pKa = 8.77IRR156 pKa = 11.84RR157 pKa = 11.84QFSIRR162 pKa = 3.34

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3749

0

3749

1174667

20

7632

313.3

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.49 ± 0.054

1.071 ± 0.016

5.119 ± 0.036

5.874 ± 0.042

3.986 ± 0.036

6.899 ± 0.044

2.515 ± 0.022

6.697 ± 0.043

4.986 ± 0.054

10.783 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.027

4.317 ± 0.039

4.341 ± 0.035

4.792 ± 0.043

5.246 ± 0.037

6.158 ± 0.034

5.309 ± 0.031

6.378 ± 0.033

1.357 ± 0.016

3.148 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski