Enterobacter phage LAU1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9WIU1|A0A6B9WIU1_9CAUD WbuC OS=Enterobacter phage LAU1 OX=2690212 PE=4 SV=1
MM1 pKa = 7.28VLNFDD6 pKa = 3.92DD7 pKa = 5.07RR8 pKa = 11.84GTVTHH13 pKa = 6.95RR14 pKa = 11.84AILGEE19 pKa = 4.2TCTVLEE25 pKa = 4.34MAAGTWHH32 pKa = 6.98AVLSLDD38 pKa = 3.03TGGIIFEE45 pKa = 4.55VKK47 pKa = 10.05HH48 pKa = 6.08GGYY51 pKa = 9.95QPVAADD57 pKa = 5.7DD58 pKa = 3.98YY59 pKa = 11.24AHH61 pKa = 7.4WAPAEE66 pKa = 4.25GEE68 pKa = 4.18PGTTEE73 pKa = 3.83LMAWYY78 pKa = 9.37AQAQVGDD85 pKa = 3.87STFAVV90 pKa = 3.71

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9WI61|A0A6B9WI61_9CAUD Tail assembly protein OS=Enterobacter phage LAU1 OX=2690212 PE=4 SV=1
MM1 pKa = 7.24EE2 pKa = 5.67RR3 pKa = 11.84KK4 pKa = 7.57TVIKK8 pKa = 10.51LSGSMAQRR16 pKa = 11.84FGRR19 pKa = 11.84THH21 pKa = 6.8RR22 pKa = 11.84RR23 pKa = 11.84ALTSASEE30 pKa = 4.23VFRR33 pKa = 11.84ALSNTIAGFDD43 pKa = 3.22AYY45 pKa = 10.87LRR47 pKa = 11.84EE48 pKa = 4.1ARR50 pKa = 11.84AKK52 pKa = 10.7GLDD55 pKa = 3.48FVIFRR60 pKa = 11.84DD61 pKa = 3.54RR62 pKa = 11.84RR63 pKa = 11.84NIGHH67 pKa = 6.54EE68 pKa = 4.17EE69 pKa = 4.04FEE71 pKa = 4.33LLGPGDD77 pKa = 4.34EE78 pKa = 4.51LRR80 pKa = 11.84IIPVIRR86 pKa = 11.84GSKK89 pKa = 9.04RR90 pKa = 11.84AGVFQALLGTALVAAAIWMPGVSIAASNLMFSVGAAMAVGGVVQMLSPQVSGLRR144 pKa = 11.84MRR146 pKa = 11.84QEE148 pKa = 4.29PDD150 pKa = 3.05NKK152 pKa = 9.92PSYY155 pKa = 11.21AFGGPVNTTASGNPVPLLYY174 pKa = 10.33GQRR177 pKa = 11.84EE178 pKa = 3.92IGGAIISAGVYY189 pKa = 10.64AEE191 pKa = 4.25DD192 pKa = 3.65QQQ194 pKa = 4.32

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

9533

55

1281

288.9

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.819 ± 0.704

0.818 ± 0.133

5.78 ± 0.31

6.189 ± 0.424

3.567 ± 0.336

7.721 ± 0.493

1.301 ± 0.238

5.549 ± 0.244

4.815 ± 0.417

8.277 ± 0.497

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.172

4.857 ± 0.248

3.797 ± 0.337

4.92 ± 0.433

5.937 ± 0.33

7.144 ± 0.266

6.294 ± 0.516

6.535 ± 0.351

1.427 ± 0.126

2.916 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski