Barley yellow dwarf virus (isolate PAV) (BYDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P09505-2|RDRP-2_BYDVP Isoform of P09505 Isoform 39 kDa protein of Putative RNA-directed RNA polymerase OS=Barley yellow dwarf virus (isolate PAV) OX=2169986 GN=ORF1/ORF2 PE=4 SV=2
MM1 pKa = 7.29FFEE4 pKa = 4.37ILIGASAKK12 pKa = 9.9AVKK15 pKa = 10.39DD16 pKa = 4.76FISHH20 pKa = 6.66CYY22 pKa = 10.06SRR24 pKa = 11.84LKK26 pKa = 10.37SIYY29 pKa = 9.71YY30 pKa = 9.79SFKK33 pKa = 9.13RR34 pKa = 11.84WLMEE38 pKa = 3.49ISGQFKK44 pKa = 10.55AHH46 pKa = 6.75DD47 pKa = 4.2AFVNMCFGHH56 pKa = 6.08MADD59 pKa = 4.13IEE61 pKa = 4.26DD62 pKa = 4.48FEE64 pKa = 6.67AEE66 pKa = 3.94LAEE69 pKa = 4.22EE70 pKa = 4.04FAEE73 pKa = 4.79RR74 pKa = 11.84EE75 pKa = 4.3DD76 pKa = 3.93EE77 pKa = 4.31VEE79 pKa = 4.12EE80 pKa = 4.29ARR82 pKa = 11.84SLLKK86 pKa = 10.96LLVAQKK92 pKa = 10.25SKK94 pKa = 11.19SGVTEE99 pKa = 3.65AWTDD103 pKa = 3.69FFTKK107 pKa = 10.38SRR109 pKa = 11.84GGVYY113 pKa = 10.38APLSCEE119 pKa = 3.84PTRR122 pKa = 11.84QEE124 pKa = 5.16LEE126 pKa = 4.27VKK128 pKa = 10.02SEE130 pKa = 3.81KK131 pKa = 10.67LEE133 pKa = 4.14RR134 pKa = 11.84LLEE137 pKa = 4.24EE138 pKa = 3.56QHH140 pKa = 5.56QFEE143 pKa = 4.32VRR145 pKa = 11.84AAKK148 pKa = 10.1KK149 pKa = 9.89YY150 pKa = 9.82IKK152 pKa = 10.21EE153 pKa = 3.96KK154 pKa = 10.45GRR156 pKa = 11.84GFINCWNDD164 pKa = 2.75LRR166 pKa = 11.84SRR168 pKa = 11.84LRR170 pKa = 11.84LVKK173 pKa = 10.26DD174 pKa = 3.8VKK176 pKa = 11.28DD177 pKa = 3.65EE178 pKa = 4.5AKK180 pKa = 10.96DD181 pKa = 3.6NARR184 pKa = 11.84AAAKK188 pKa = 10.08IGAEE192 pKa = 3.9MFAPVDD198 pKa = 3.85VQDD201 pKa = 4.83LYY203 pKa = 12.01SFTEE207 pKa = 4.12VKK209 pKa = 10.42KK210 pKa = 11.31VEE212 pKa = 4.03TGLMKK217 pKa = 10.55EE218 pKa = 4.31VVKK221 pKa = 10.66EE222 pKa = 4.01KK223 pKa = 10.85NGEE226 pKa = 4.13EE227 pKa = 4.15EE228 pKa = 4.05KK229 pKa = 10.79HH230 pKa = 6.15LEE232 pKa = 4.42PIMEE236 pKa = 4.39EE237 pKa = 3.79VRR239 pKa = 11.84SIKK242 pKa = 9.8DD243 pKa = 3.03TAEE246 pKa = 3.95ARR248 pKa = 11.84DD249 pKa = 3.8AASTWITEE257 pKa = 4.33TVKK260 pKa = 11.0LKK262 pKa = 10.83NATLNADD269 pKa = 4.14EE270 pKa = 5.48LSLATIARR278 pKa = 11.84YY279 pKa = 9.55VEE281 pKa = 3.98NVGDD285 pKa = 4.18KK286 pKa = 10.66FKK288 pKa = 11.42LDD290 pKa = 3.69IASKK294 pKa = 9.49TYY296 pKa = 10.25LKK298 pKa = 10.28QVASMSVPIPTNKK311 pKa = 10.06DD312 pKa = 2.74IKK314 pKa = 10.87LKK316 pKa = 10.16MVLQSPEE323 pKa = 3.1ARR325 pKa = 11.84ARR327 pKa = 11.84RR328 pKa = 11.84EE329 pKa = 3.79RR330 pKa = 11.84MDD332 pKa = 3.34VLDD335 pKa = 3.87SVGFF339 pKa = 3.75

Molecular weight: 38.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P09513|MVP_BYDVP Movement protein OS=Barley yellow dwarf virus (isolate PAV) OX=2169986 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.32NSVGRR6 pKa = 11.84RR7 pKa = 11.84GPRR10 pKa = 11.84RR11 pKa = 11.84ANQNGTRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84TVRR26 pKa = 11.84PVVVVQPNRR35 pKa = 11.84AGPRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84NGRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 10.36GRR48 pKa = 11.84GGANFVFRR56 pKa = 11.84PTGGTEE62 pKa = 3.86VFVFSVDD69 pKa = 3.29NLKK72 pKa = 11.06ANSSGAIKK80 pKa = 10.42FGPSLSQCPALSDD93 pKa = 4.84GILKK97 pKa = 10.33SYY99 pKa = 10.27HH100 pKa = 6.5RR101 pKa = 11.84YY102 pKa = 9.53KK103 pKa = 9.44ITSIRR108 pKa = 11.84VEE110 pKa = 4.62FKK112 pKa = 10.49SHH114 pKa = 6.49ASANTAGAIFIEE126 pKa = 5.16LDD128 pKa = 3.7TACKK132 pKa = 9.92QSALGSYY139 pKa = 9.83INSFTISKK147 pKa = 8.05TASKK151 pKa = 9.21TFRR154 pKa = 11.84SEE156 pKa = 4.17AINGKK161 pKa = 7.99EE162 pKa = 4.03FQEE165 pKa = 4.27STIDD169 pKa = 3.81QFWMLYY175 pKa = 8.9KK176 pKa = 10.97ANGTTTDD183 pKa = 3.17TAGQFIITMSVSLMTAKK200 pKa = 10.59

Molecular weight: 22.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

2327

55

867

332.4

37.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.778 ± 0.49

1.418 ± 0.251

4.641 ± 0.348

7.434 ± 0.866

4.083 ± 0.381

5.887 ± 0.468

1.676 ± 0.316

5.329 ± 0.274

7.134 ± 0.998

7.005 ± 0.865

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.3

4.211 ± 0.402

4.856 ± 1.04

3.997 ± 0.677

6.489 ± 0.591

8.337 ± 0.675

6.231 ± 1.011

6.661 ± 0.393

0.945 ± 0.095

3.309 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski