Oceanithermus profundus (strain DSM 14977 / NBRC 100410 / VKM B-2274 / 506)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Oceanithermus; Oceanithermus profundus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4UA54|E4UA54_OCEP5 Small multidrug resistance protein OS=Oceanithermus profundus (strain DSM 14977 / NBRC 100410 / VKM B-2274 / 506) OX=670487 GN=Ocepr_1980 PE=3 SV=1
MM1 pKa = 7.2LTHH4 pKa = 6.37LRR6 pKa = 11.84YY7 pKa = 9.35RR8 pKa = 11.84VRR10 pKa = 11.84FAAFVLGVMGLLAACSQPAGGAGGFSLSLEE40 pKa = 4.29PTSVTVAAGGSTTVRR55 pKa = 11.84LDD57 pKa = 3.17VGSEE61 pKa = 4.02GGFSGEE67 pKa = 3.72VSLALEE73 pKa = 4.49GLAGATVSPARR84 pKa = 11.84VQVPGGPYY92 pKa = 9.73TLTLAVDD99 pKa = 4.05DD100 pKa = 4.59SVAPGSYY107 pKa = 10.88DD108 pKa = 3.28LALVGASGSLMGRR121 pKa = 11.84ATFTLAVTDD130 pKa = 3.75ATPDD134 pKa = 3.41FEE136 pKa = 4.93LALSAVDD143 pKa = 4.48LAADD147 pKa = 3.96PGGSVGTALTVTPSGGFAGTLTLSLVDD174 pKa = 4.99ASDD177 pKa = 3.7APAAGFTLVPASLYY191 pKa = 10.73VSGIVQQPLSIQVAGDD207 pKa = 3.72VGPGTYY213 pKa = 10.18PLRR216 pKa = 11.84LKK218 pKa = 9.66VTGDD222 pKa = 3.52GVTHH226 pKa = 6.02YY227 pKa = 11.21ADD229 pKa = 3.69LAVTVSGLEE238 pKa = 3.83LALTSTEE245 pKa = 4.31LYY247 pKa = 10.5APQGGTATATLLVNAVGVSGDD268 pKa = 3.64LALALASADD277 pKa = 3.74GGPAPTGLALQPSSVAVPGGPYY299 pKa = 10.55ALSLSVDD306 pKa = 3.27AGVATGSYY314 pKa = 11.07DD315 pKa = 4.0LLLKK319 pKa = 9.68GTLGSFEE326 pKa = 4.43RR327 pKa = 11.84SVAFTLTVQPPPEE340 pKa = 4.24FTTSVSPDD348 pKa = 3.21ALTVEE353 pKa = 4.72VGSSGTLYY361 pKa = 11.19LEE363 pKa = 4.51LTFQADD369 pKa = 3.7FNGDD373 pKa = 2.69IGFALEE379 pKa = 4.41LADD382 pKa = 5.49GSAAPAGLALDD393 pKa = 3.82PTVVSMAANSGDD405 pKa = 3.66TNYY408 pKa = 10.38VALDD412 pKa = 3.67LAVADD417 pKa = 4.13TMAPGTYY424 pKa = 10.13ALRR427 pKa = 11.84VRR429 pKa = 11.84ASSSNTTQYY438 pKa = 11.73AGFSLTVPVPPDD450 pKa = 3.31FTLSLDD456 pKa = 3.39PTIADD461 pKa = 3.56VVAGGSTTVRR471 pKa = 11.84LDD473 pKa = 3.58AVPHH477 pKa = 6.02NGFAGVVQLSLVDD490 pKa = 4.01SSNGAAPSGMTLEE503 pKa = 4.36PTSVDD508 pKa = 3.37LSGGAVSQTLTLRR521 pKa = 11.84VDD523 pKa = 3.25EE524 pKa = 4.78GVLVDD529 pKa = 5.05RR530 pKa = 11.84YY531 pKa = 9.6HH532 pKa = 6.16LQVVGSGGGTTQTANLSLSVQDD554 pKa = 4.7FSLSFGTTTPSLAADD569 pKa = 3.53QGGSATTTLTVQVSQPDD586 pKa = 4.08TYY588 pKa = 10.81STFPGPVALEE598 pKa = 4.48LATQDD603 pKa = 4.55GSPLPPGLTLSPVSVDD619 pKa = 3.55LSSGYY624 pKa = 11.13VNVNLTVTADD634 pKa = 3.09ASTPPGSYY642 pKa = 10.67ALMVIGRR649 pKa = 11.84AGGTARR655 pKa = 11.84AAAFGLNVRR664 pKa = 11.84GFDD667 pKa = 3.56VALGASEE674 pKa = 4.06LAFWTEE680 pKa = 3.9GSGDD684 pKa = 3.9LGLTLTPGGGFDD696 pKa = 3.76GTVSLSLEE704 pKa = 4.29AQDD707 pKa = 5.47GSAAPAGLTLSPSSVSVSSTTSVTLSVAADD737 pKa = 3.39ASVAAGVYY745 pKa = 9.13PLRR748 pKa = 11.84LKK750 pKa = 10.6AVSGSIVHH758 pKa = 6.16YY759 pKa = 11.12ADD761 pKa = 3.45FTLKK765 pKa = 10.95VGDD768 pKa = 4.21FDD770 pKa = 5.0LALDD774 pKa = 4.22ASSLAVWQNDD784 pKa = 2.89RR785 pKa = 11.84SGFGVTVTPSGGFAGTVNLYY805 pKa = 9.7LAPQVGATGPTGVSLDD821 pKa = 3.78PSALDD826 pKa = 3.27VSGGVSQTVNVVAADD841 pKa = 3.6AASPGSFAMQLEE853 pKa = 3.89ARR855 pKa = 11.84AYY857 pKa = 9.07LGGVARR863 pKa = 11.84SRR865 pKa = 11.84TVPFTLEE872 pKa = 3.83VKK874 pKa = 10.56GFLVEE879 pKa = 4.56LSEE882 pKa = 5.08DD883 pKa = 3.58NYY885 pKa = 11.06FEE887 pKa = 4.63ARR889 pKa = 11.84GGSASGQLTLTAYY902 pKa = 10.29GLNDD906 pKa = 3.6VVALALLDD914 pKa = 3.93PSGAAPAGIALAPASVAASDD934 pKa = 3.87GAGTHH939 pKa = 7.18TITIGTDD946 pKa = 2.82ANVDD950 pKa = 3.36YY951 pKa = 11.17DD952 pKa = 3.73RR953 pKa = 11.84TYY955 pKa = 11.72GLDD958 pKa = 4.65LVATWGSLEE967 pKa = 3.91RR968 pKa = 11.84RR969 pKa = 11.84LAINLTVYY977 pKa = 10.86RR978 pKa = 11.84LTEE981 pKa = 3.77YY982 pKa = 9.58WVPRR986 pKa = 11.84DD987 pKa = 3.56PGTDD991 pKa = 3.2YY992 pKa = 11.48DD993 pKa = 3.99LWGVAYY999 pKa = 10.41GEE1001 pKa = 4.57VNGSGLFVAVGGGQLSLNGSSYY1023 pKa = 11.0GVAATSTDD1031 pKa = 3.4GASWSLTEE1039 pKa = 5.83DD1040 pKa = 3.0ISSNPLLGIGYY1051 pKa = 10.24GGGTFVAVGRR1061 pKa = 11.84ACEE1064 pKa = 3.72IVTYY1068 pKa = 10.57DD1069 pKa = 3.21GTGWTTRR1076 pKa = 11.84ANPDD1080 pKa = 3.54AYY1082 pKa = 10.89CSANLNDD1089 pKa = 3.49VAYY1092 pKa = 10.68SGTRR1096 pKa = 11.84FVAVGDD1102 pKa = 3.85SGTVLWSNDD1111 pKa = 2.92GASWSSATSGVSGDD1125 pKa = 4.63LYY1127 pKa = 11.14AITYY1131 pKa = 10.39GNGTFVAVGAGGTVLTSTDD1150 pKa = 3.38GLSWSAQASGTTADD1164 pKa = 4.32LSGIAYY1170 pKa = 10.33GDD1172 pKa = 3.61GTFVAVGAGGTVLTSTDD1189 pKa = 3.33GASWSLGDD1197 pKa = 5.07LGTSGDD1203 pKa = 3.94ATGVAYY1209 pKa = 9.8GYY1211 pKa = 10.82DD1212 pKa = 3.53WDD1214 pKa = 4.06NVGVFVVTTNEE1225 pKa = 4.18PNTGIHH1231 pKa = 6.03TSSDD1235 pKa = 3.4AGSSWSSQSSGGLGDD1250 pKa = 3.9ALEE1253 pKa = 4.16AAGYY1257 pKa = 8.59GDD1259 pKa = 3.54HH1260 pKa = 6.48VFVVVGQLGALGTSPP1275 pKa = 4.6

Molecular weight: 126.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4U4H4|E4U4H4_OCEP5 Thymidine kinase OS=Oceanithermus profundus (strain DSM 14977 / NBRC 100410 / VKM B-2274 / 506) OX=670487 GN=tdk PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.45RR3 pKa = 11.84TWQPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84AKK14 pKa = 8.56THH16 pKa = 5.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 8.77VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2372

0

2372

750473

30

2686

316.4

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.74 ± 0.073

0.433 ± 0.015

4.711 ± 0.038

7.414 ± 0.063

3.599 ± 0.032

9.096 ± 0.052

1.877 ± 0.021

3.459 ± 0.043

2.928 ± 0.044

11.98 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.768 ± 0.021

1.943 ± 0.027

6.034 ± 0.039

2.561 ± 0.025

8.171 ± 0.073

3.867 ± 0.031

4.476 ± 0.041

8.387 ± 0.052

1.701 ± 0.027

2.855 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski