Listeria phage vB_LmoS_293

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5CTY6|A0A0B5CTY6_9CAUD Uncharacterized protein OS=Listeria phage vB_LmoS_293 OX=1591073 GN=SE25_060 PE=4 SV=1
MM1 pKa = 7.15NFKK4 pKa = 10.84VGDD7 pKa = 3.58KK8 pKa = 9.08VTWIKK13 pKa = 10.99DD14 pKa = 3.33KK15 pKa = 11.09NVSGKK20 pKa = 9.68IVSINDD26 pKa = 2.99FRR28 pKa = 11.84PPEE31 pKa = 4.02MEE33 pKa = 4.11CAVDD37 pKa = 5.19FGFNDD42 pKa = 3.17VAFCSRR48 pKa = 11.84EE49 pKa = 3.85EE50 pKa = 4.04LVLDD54 pKa = 4.51EE55 pKa = 5.0SATKK59 pKa = 10.27QEE61 pKa = 4.17SVNCKK66 pKa = 9.98EE67 pKa = 4.1CDD69 pKa = 3.45EE70 pKa = 4.87TFTQYY75 pKa = 11.66DD76 pKa = 4.01EE77 pKa = 5.86IIVFDD82 pKa = 3.95GNAWHH87 pKa = 7.41KK88 pKa = 11.18DD89 pKa = 3.58CLDD92 pKa = 4.09KK93 pKa = 11.34FPATYY98 pKa = 9.99AYY100 pKa = 10.33YY101 pKa = 10.56EE102 pKa = 4.26KK103 pKa = 11.35GEE105 pKa = 4.43DD106 pKa = 3.75GEE108 pKa = 5.77FINTDD113 pKa = 3.45DD114 pKa = 4.79NADD117 pKa = 3.67FDD119 pKa = 4.48GDD121 pKa = 3.8AYY123 pKa = 10.62EE124 pKa = 5.12HH125 pKa = 6.93FDD127 pKa = 3.84GLEE130 pKa = 3.88EE131 pKa = 4.03EE132 pKa = 4.61

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5CU49|A0A0B5CU49_9CAUD Uncharacterized protein OS=Listeria phage vB_LmoS_293 OX=1591073 GN=SE25_056 PE=4 SV=1
MM1 pKa = 7.43GKK3 pKa = 10.33YY4 pKa = 8.07YY5 pKa = 10.08WHH7 pKa = 6.92VSRR10 pKa = 11.84LGGKK14 pKa = 6.75PTEE17 pKa = 3.88IRR19 pKa = 11.84HH20 pKa = 5.44YY21 pKa = 10.3NHH23 pKa = 5.92ITKK26 pKa = 9.45MYY28 pKa = 10.54KK29 pKa = 10.34FILRR33 pKa = 11.84NPAMFKK39 pKa = 10.89DD40 pKa = 3.79KK41 pKa = 10.4TLTIYY46 pKa = 11.02DD47 pKa = 3.5HH48 pKa = 7.08AKK50 pKa = 10.36AVTNMTFNEE59 pKa = 3.72IKK61 pKa = 10.7YY62 pKa = 9.87RR63 pKa = 11.84ASLNLCEE70 pKa = 4.31TVEE73 pKa = 3.87RR74 pKa = 11.84RR75 pKa = 11.84YY76 pKa = 10.5VLSLTQRR83 pKa = 11.84LKK85 pKa = 11.07EE86 pKa = 4.04EE87 pKa = 4.13QAA89 pKa = 3.46

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12636

32

1788

175.5

19.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.914 ± 0.893

0.728 ± 0.136

5.888 ± 0.35

7.985 ± 0.582

3.909 ± 0.243

5.69 ± 0.376

1.219 ± 0.175

6.838 ± 0.238

9.196 ± 0.396

8.143 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.146

6.323 ± 0.224

2.453 ± 0.21

3.538 ± 0.231

3.498 ± 0.368

6.371 ± 0.414

6.117 ± 0.211

6.569 ± 0.226

1.108 ± 0.142

3.878 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski