Azobacteroides pseudotrichonymphae genomovar. CFP2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidales incertae sedis; Candidatus Azobacteroides; Candidatus Azobacteroides pseudotrichonymphae

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6YS48|B6YS48_AZOPC M22 family peptidase OS=Azobacteroides pseudotrichonymphae genomovar. CFP2 OX=511995 GN=CFPG_757 PE=4 SV=1
MM1 pKa = 7.23YY2 pKa = 7.73WTLEE6 pKa = 4.06LVSYY10 pKa = 11.06LEE12 pKa = 4.33DD13 pKa = 4.15APWPATKK20 pKa = 10.41SEE22 pKa = 4.57LIDD25 pKa = 3.56YY26 pKa = 9.21AVRR29 pKa = 11.84ACCPDD34 pKa = 3.41EE35 pKa = 4.95VIEE38 pKa = 4.22NLQEE42 pKa = 4.08LEE44 pKa = 4.32EE45 pKa = 4.16EE46 pKa = 4.22NEE48 pKa = 4.07IYY50 pKa = 10.66EE51 pKa = 4.56SIEE54 pKa = 4.28DD55 pKa = 3.58VWPDD59 pKa = 3.71YY60 pKa = 10.43PSKK63 pKa = 11.26DD64 pKa = 2.92DD65 pKa = 3.94FLFNEE70 pKa = 4.77DD71 pKa = 3.58EE72 pKa = 4.45YY73 pKa = 12.0

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B6YQ75|RL14_AZOPC 50S ribosomal protein L14 OS=Azobacteroides pseudotrichonymphae genomovar. CFP2 OX=511995 GN=rplN PE=3 SV=1
MM1 pKa = 7.72AVRR4 pKa = 11.84ILGVDD9 pKa = 4.0LPQNKK14 pKa = 9.41RR15 pKa = 11.84GEE17 pKa = 3.94IALTYY22 pKa = 9.89IYY24 pKa = 10.62GIGCGLSSKK33 pKa = 10.32ILTEE37 pKa = 4.07AGIDD41 pKa = 3.23RR42 pKa = 11.84DD43 pKa = 4.0TRR45 pKa = 11.84IKK47 pKa = 10.86DD48 pKa = 3.18WTDD51 pKa = 3.08VQVAAVRR58 pKa = 11.84EE59 pKa = 4.24IISRR63 pKa = 11.84NFKK66 pKa = 10.89VEE68 pKa = 3.33GDD70 pKa = 3.73LRR72 pKa = 11.84SEE74 pKa = 3.57IQLNIKK80 pKa = 10.03RR81 pKa = 11.84LMDD84 pKa = 3.77IGCYY88 pKa = 9.44RR89 pKa = 11.84GIRR92 pKa = 11.84HH93 pKa = 7.08RR94 pKa = 11.84IGLPLRR100 pKa = 11.84GQSTKK105 pKa = 10.94NNARR109 pKa = 11.84TRR111 pKa = 11.84KK112 pKa = 9.26GKK114 pKa = 10.5RR115 pKa = 11.84KK116 pKa = 7.5TVANKK121 pKa = 10.07KK122 pKa = 9.95RR123 pKa = 11.84VTKK126 pKa = 10.74

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

847

0

847

285234

50

1508

336.8

38.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.637 ± 0.076

1.379 ± 0.033

4.87 ± 0.063

6.608 ± 0.068

4.817 ± 0.061

6.228 ± 0.064

1.956 ± 0.033

9.701 ± 0.072

8.048 ± 0.069

9.343 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.031

5.627 ± 0.063

3.252 ± 0.036

3.381 ± 0.05

4.474 ± 0.054

6.358 ± 0.046

4.945 ± 0.046

6.018 ± 0.071

0.941 ± 0.029

4.057 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski