Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; Potato leafroll virus

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P17525-2|MCAPS-2_PLRV1 Isoform of P17525 Isoform 2 of Minor capsid protein P3-RTD OS=Potato leafroll virus (strain Potato/Scotland/strain 1/1984) OX=12046 GN=ORF3/ORF5 PE=4 SV=2
VV1 pKa = 7.71DD2 pKa = 4.22SGSEE6 pKa = 4.06PSPSPQPTPTPTPQKK21 pKa = 9.54HH22 pKa = 5.31EE23 pKa = 4.05RR24 pKa = 11.84FIAYY28 pKa = 9.71VGIPMLTIQARR39 pKa = 11.84EE40 pKa = 3.85NDD42 pKa = 3.67DD43 pKa = 3.94QIILGSLGSQRR54 pKa = 11.84MKK56 pKa = 10.93YY57 pKa = 9.9IEE59 pKa = 5.51DD60 pKa = 3.36EE61 pKa = 4.18NQNYY65 pKa = 8.21TKK67 pKa = 10.51FSSEE71 pKa = 4.59YY72 pKa = 8.59YY73 pKa = 9.92SQSSMQAVPMYY84 pKa = 9.89YY85 pKa = 10.3FNVPKK90 pKa = 10.36GQWSVDD96 pKa = 3.39ISCEE100 pKa = 4.18GYY102 pKa = 10.21QPTSSTSDD110 pKa = 3.55PNRR113 pKa = 11.84GRR115 pKa = 11.84SDD117 pKa = 3.4GMIAYY122 pKa = 10.35SNADD126 pKa = 2.79SDD128 pKa = 4.02YY129 pKa = 9.5WNVGEE134 pKa = 4.53ADD136 pKa = 3.49GVKK139 pKa = 9.88ISKK142 pKa = 9.59LRR144 pKa = 11.84NDD146 pKa = 2.86NTYY149 pKa = 10.53RR150 pKa = 11.84QGHH153 pKa = 6.79PEE155 pKa = 4.05LEE157 pKa = 4.1INSCHH162 pKa = 5.84FRR164 pKa = 11.84EE165 pKa = 4.68GQLLEE170 pKa = 4.26RR171 pKa = 11.84DD172 pKa = 3.63ATISFHH178 pKa = 8.31VEE180 pKa = 3.32APTDD184 pKa = 3.4GRR186 pKa = 11.84FFLVGPAIQKK196 pKa = 5.49TAKK199 pKa = 9.33YY200 pKa = 9.73NYY202 pKa = 8.05TISYY206 pKa = 10.17GDD208 pKa = 3.06WTDD211 pKa = 3.7RR212 pKa = 11.84DD213 pKa = 3.91MEE215 pKa = 4.99LGLITVVLDD224 pKa = 3.29EE225 pKa = 4.77HH226 pKa = 8.61LEE228 pKa = 4.34GTGSANRR235 pKa = 11.84VRR237 pKa = 11.84RR238 pKa = 11.84PPRR241 pKa = 11.84EE242 pKa = 3.32GHH244 pKa = 6.13TYY246 pKa = 8.54MASPHH251 pKa = 5.77EE252 pKa = 4.38PEE254 pKa = 4.9GKK256 pKa = 9.47PVGNKK261 pKa = 9.3PRR263 pKa = 11.84DD264 pKa = 3.45EE265 pKa = 4.47TPIQTQEE272 pKa = 4.03RR273 pKa = 11.84QPDD276 pKa = 3.65QTPSDD281 pKa = 4.17DD282 pKa = 3.7VSDD285 pKa = 4.08AGSVNSGGPTEE296 pKa = 4.13SLRR299 pKa = 11.84LEE301 pKa = 4.24FGVNSDD307 pKa = 3.13STYY310 pKa = 11.27DD311 pKa = 3.5ATVDD315 pKa = 3.44GTDD318 pKa = 3.38WPRR321 pKa = 11.84IPPPRR326 pKa = 11.84HH327 pKa = 5.24PPEE330 pKa = 4.29PRR332 pKa = 11.84VSGNSRR338 pKa = 11.84TVTDD342 pKa = 5.0FSSKK346 pKa = 11.18ADD348 pKa = 4.0LLEE351 pKa = 4.27NWDD354 pKa = 4.58AEE356 pKa = 4.39HH357 pKa = 7.23FDD359 pKa = 3.6PGYY362 pKa = 10.6SKK364 pKa = 11.2EE365 pKa = 4.24DD366 pKa = 3.24VAAATIIAHH375 pKa = 6.99GSIQDD380 pKa = 3.53GRR382 pKa = 11.84SMLEE386 pKa = 3.66KK387 pKa = 10.35RR388 pKa = 11.84EE389 pKa = 4.03EE390 pKa = 3.98NVKK393 pKa = 10.85NKK395 pKa = 8.99TSSWKK400 pKa = 9.93PPSLKK405 pKa = 10.48AVSPAIAKK413 pKa = 9.51LRR415 pKa = 11.84SIRR418 pKa = 11.84KK419 pKa = 7.69SQPLEE424 pKa = 4.04GGTLNKK430 pKa = 10.38DD431 pKa = 3.0ATDD434 pKa = 4.12GVSSIGSGSLTGGTLKK450 pKa = 10.59RR451 pKa = 11.84KK452 pKa = 8.53ATIEE456 pKa = 3.84EE457 pKa = 4.38RR458 pKa = 11.84LLQTLTTEE466 pKa = 3.67QRR468 pKa = 11.84LWYY471 pKa = 10.26EE472 pKa = 4.08NFKK475 pKa = 9.32KK476 pKa = 9.31TNPPAATQWLFEE488 pKa = 4.28YY489 pKa = 10.49QPPPQVDD496 pKa = 3.24RR497 pKa = 11.84NIAEE501 pKa = 4.33KK502 pKa = 10.39PFQGRR507 pKa = 11.84KK508 pKa = 8.39

Molecular weight: 56.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C769|P6_PLRV1 Uncharacterized protein P6 OS=Potato leafroll virus (strain Potato/Scotland/strain 1/1984) OX=12046 PE=4 SV=1
MM1 pKa = 7.19TPMRR5 pKa = 11.84ITVWRR10 pKa = 11.84EE11 pKa = 3.06RR12 pKa = 11.84LQQMRR17 pKa = 11.84PQRR20 pKa = 11.84KK21 pKa = 8.48LLKK24 pKa = 8.83QTQQRR29 pKa = 11.84RR30 pKa = 11.84LLHH33 pKa = 5.63QLQQRR38 pKa = 11.84KK39 pKa = 8.55LLL41 pKa = 4.03

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3764

41

1062

376.4

41.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.333 ± 0.892

1.328 ± 0.3

4.145 ± 0.554

6.376 ± 0.463

3.666 ± 0.332

6.961 ± 0.341

2.046 ± 0.203

4.171 ± 0.24

5.898 ± 0.489

8.502 ± 1.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.171

4.304 ± 0.179

6.35 ± 0.622

4.623 ± 0.537

6.27 ± 0.694

9.458 ± 0.49

6.536 ± 0.309

5.074 ± 0.341

1.86 ± 0.225

3.162 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski