Corynebacterium halotolerans YIM 70093 = DSM 44683

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium halotolerans

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M1MYC0|M1MYC0_9CORY Membrane protein OS=Corynebacterium halotolerans YIM 70093 = DSM 44683 OX=1121362 GN=A605_08660 PE=4 SV=1
MM1 pKa = 7.8SGLTLAATGVLAPTVTAQEE20 pKa = 4.32DD21 pKa = 4.24TATTAWEE28 pKa = 3.88EE29 pKa = 3.64TAYY32 pKa = 10.37RR33 pKa = 11.84GSVEE37 pKa = 4.68VVDD40 pKa = 4.52EE41 pKa = 4.16QDD43 pKa = 5.11DD44 pKa = 4.26EE45 pKa = 4.49NTLEE49 pKa = 4.13GVVFEE54 pKa = 4.84DD55 pKa = 3.94TNKK58 pKa = 10.3NSQRR62 pKa = 11.84DD63 pKa = 3.62EE64 pKa = 4.42GEE66 pKa = 3.69PGIPGVSVSNGRR78 pKa = 11.84TIVTTDD84 pKa = 2.67AEE86 pKa = 4.49GRR88 pKa = 11.84YY89 pKa = 8.76EE90 pKa = 4.62LQVDD94 pKa = 3.88DD95 pKa = 4.28NTTVFITQPAGYY107 pKa = 9.0QVPVDD112 pKa = 4.28EE113 pKa = 5.13NNVAQFFYY121 pKa = 10.87NHH123 pKa = 5.73VPEE126 pKa = 5.05GSPDD130 pKa = 3.27LRR132 pKa = 11.84YY133 pKa = 10.55SGIEE137 pKa = 3.79PTGPLPDD144 pKa = 3.76AVNFPLSPSEE154 pKa = 4.02GTASPEE160 pKa = 3.94QNCVIGGDD168 pKa = 3.41IQTYY172 pKa = 5.57TAEE175 pKa = 4.11EE176 pKa = 3.92VEE178 pKa = 4.48YY179 pKa = 11.02ARR181 pKa = 11.84NGAFADD187 pKa = 4.12LAARR191 pKa = 11.84DD192 pKa = 4.47DD193 pKa = 4.05YY194 pKa = 11.8ANCGALFIGDD204 pKa = 3.83VVGDD208 pKa = 4.43DD209 pKa = 3.6LSLYY213 pKa = 10.08PDD215 pKa = 3.53VKK217 pKa = 10.75EE218 pKa = 3.91LTGMLNGPARR228 pKa = 11.84FLPGNHH234 pKa = 7.08DD235 pKa = 4.3LDD237 pKa = 4.62FDD239 pKa = 5.63ASDD242 pKa = 4.07EE243 pKa = 4.03DD244 pKa = 4.42HH245 pKa = 7.2KK246 pKa = 11.4FDD248 pKa = 3.75TYY250 pKa = 10.89RR251 pKa = 11.84SHH253 pKa = 7.73FGPEE257 pKa = 4.15YY258 pKa = 10.46YY259 pKa = 10.81SFDD262 pKa = 3.26VGNAHH267 pKa = 6.16VVALEE272 pKa = 4.03SVQYY276 pKa = 10.18PLEE279 pKa = 5.54DD280 pKa = 3.88GDD282 pKa = 4.76GYY284 pKa = 11.56NGAIDD289 pKa = 5.05DD290 pKa = 4.69EE291 pKa = 4.28QLEE294 pKa = 4.4WLRR297 pKa = 11.84QDD299 pKa = 3.23IAHH302 pKa = 6.12TPEE305 pKa = 4.88DD306 pKa = 4.05KK307 pKa = 11.18LIVLATHH314 pKa = 6.08VPLMSFADD322 pKa = 3.61QGSDD326 pKa = 2.99VHH328 pKa = 8.11QIDD331 pKa = 3.85QVQEE335 pKa = 3.61IYY337 pKa = 10.72EE338 pKa = 4.31IIGDD342 pKa = 3.93RR343 pKa = 11.84EE344 pKa = 4.21AIAVSGHH351 pKa = 3.93THH353 pKa = 6.69ALANMRR359 pKa = 11.84EE360 pKa = 4.07GDD362 pKa = 4.2SLEE365 pKa = 4.14GWNEE369 pKa = 3.84LYY371 pKa = 10.66GVEE374 pKa = 4.51EE375 pKa = 4.65LPFTHH380 pKa = 6.63IVAGAISGDD389 pKa = 3.6WYY391 pKa = 11.09SGEE394 pKa = 4.2TTEE397 pKa = 5.2NGYY400 pKa = 8.81PQAVGRR406 pKa = 11.84DD407 pKa = 3.51GSLPGVLTLDD417 pKa = 3.19IDD419 pKa = 3.93GSEE422 pKa = 3.87VSEE425 pKa = 4.96FYY427 pKa = 9.99TVRR430 pKa = 11.84GEE432 pKa = 4.19DD433 pKa = 3.37QSTQMALSLNTPAYY447 pKa = 9.91RR448 pKa = 11.84DD449 pKa = 3.47WYY451 pKa = 10.08EE452 pKa = 4.67DD453 pKa = 3.69NIDD456 pKa = 3.69NAGEE460 pKa = 4.07AEE462 pKa = 4.37EE463 pKa = 4.53FADD466 pKa = 3.67PLVVPEE472 pKa = 5.06DD473 pKa = 3.84EE474 pKa = 5.1VAEE477 pKa = 4.33TWLTTNFWMGSTGSTVEE494 pKa = 3.71VSIDD498 pKa = 3.18GGEE501 pKa = 3.8AVAAEE506 pKa = 4.67RR507 pKa = 11.84TQQMDD512 pKa = 3.76GEE514 pKa = 4.64VPNVGAEE521 pKa = 3.94WSDD524 pKa = 3.44PAAVQQQLVHH534 pKa = 6.47GGSVADD540 pKa = 4.32RR541 pKa = 11.84AAHH544 pKa = 6.53IWRR547 pKa = 11.84LPLPADD553 pKa = 4.37LGAGEE558 pKa = 4.37HH559 pKa = 5.13TAEE562 pKa = 4.07VTATDD567 pKa = 3.71VHH569 pKa = 6.12GHH571 pKa = 6.59EE572 pKa = 4.46YY573 pKa = 10.7TEE575 pKa = 4.27TLTFTVSPVEE585 pKa = 4.38GGDD588 pKa = 4.58DD589 pKa = 3.88NGDD592 pKa = 4.14DD593 pKa = 3.9DD594 pKa = 4.95TEE596 pKa = 4.91APSSGLSSLSSNLSSAGSLFF616 pKa = 3.54

Molecular weight:
66.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M1N1M5|M1N1M5_9CORY Uncharacterized protein OS=Corynebacterium halotolerans YIM 70093 = DSM 44683 OX=1121362 GN=A605_14212 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84TRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.73GRR42 pKa = 11.84AKK44 pKa = 9.63LTAA47 pKa = 4.21

Molecular weight:
5.51 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2853

0

2853

924155

33

3023

323.9

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.941 ± 0.06

0.601 ± 0.011

6.302 ± 0.042

6.449 ± 0.048

3.137 ± 0.026

9.066 ± 0.044

2.259 ± 0.024

4.513 ± 0.034

2.235 ± 0.036

10.062 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.098 ± 0.02

2.371 ± 0.024

5.402 ± 0.036

2.901 ± 0.024

7.035 ± 0.043

5.242 ± 0.03

6.331 ± 0.032

8.576 ± 0.047

1.469 ± 0.021

2.01 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski