Cellulophaga phage phi39:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A2W2|S0A2W2_9CAUD DUF5675 domain-containing protein OS=Cellulophaga phage phi39:1 OX=1327993 GN=Phi39:1_gp30 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84KK3 pKa = 10.09LILFFGLIFCLEE15 pKa = 3.82MAAQVEE21 pKa = 4.39QVEE24 pKa = 4.45AVVLAKK30 pKa = 10.57KK31 pKa = 8.94STAEE35 pKa = 3.94LNALPSRR42 pKa = 11.84LKK44 pKa = 10.72QIGGIYY50 pKa = 10.14FDD52 pKa = 3.55VTLDD56 pKa = 6.03DD57 pKa = 4.05FVTWNGSNFVDD68 pKa = 5.46FSGEE72 pKa = 4.18TYY74 pKa = 10.46IFTGGISEE82 pKa = 4.49SGGTVTLEE90 pKa = 3.98NEE92 pKa = 3.7YY93 pKa = 9.11TAAEE97 pKa = 4.09KK98 pKa = 10.99SKK100 pKa = 11.04VSNLPNDD107 pKa = 3.34QSAVNEE113 pKa = 4.36TTVKK117 pKa = 10.33FDD119 pKa = 3.82KK120 pKa = 11.34NNANTFKK127 pKa = 10.7IKK129 pKa = 9.62TADD132 pKa = 3.54DD133 pKa = 3.76DD134 pKa = 4.06AAADD138 pKa = 4.01VILTINDD145 pKa = 3.68DD146 pKa = 3.77DD147 pKa = 4.28TPGGVEE153 pKa = 4.0YY154 pKa = 10.45FDD156 pKa = 4.78EE157 pKa = 4.52YY158 pKa = 11.55VEE160 pKa = 4.67EE161 pKa = 4.08ISGAVSDD168 pKa = 4.53GNLTITLGRR177 pKa = 11.84TNLSDD182 pKa = 5.27LISAAIPLPSPTGSGSYY199 pKa = 10.09SAGTGLSLGGSVFSLAPSVQSDD221 pKa = 3.21IDD223 pKa = 3.84SKK225 pKa = 11.4LNKK228 pKa = 10.2GLTNLGGTLLDD239 pKa = 3.73VKK241 pKa = 10.82SDD243 pKa = 3.21VGFRR247 pKa = 11.84FQPNEE252 pKa = 3.94GLSGFLVTPTLTSFLMGTADD272 pKa = 3.9LSKK275 pKa = 8.39MTSFSLTADD284 pKa = 3.41YY285 pKa = 10.85FRR287 pKa = 11.84TNLSDD292 pKa = 4.55AKK294 pKa = 10.54ILAQGDD300 pKa = 4.07DD301 pKa = 3.27SMLTYY306 pKa = 10.95GFVKK310 pKa = 10.56NYY312 pKa = 10.28FLTKK316 pKa = 10.17DD317 pKa = 3.21AAAALYY323 pKa = 10.5APIGSSTGGGDD334 pKa = 4.37VDD336 pKa = 5.94DD337 pKa = 6.09ADD339 pKa = 4.87ADD341 pKa = 4.12PLNEE345 pKa = 3.9IQTLSVSGNTVTLSKK360 pKa = 11.06DD361 pKa = 2.61GGAFVIPSGGINQTIGNSVLTLKK384 pKa = 11.05GITQGDD390 pKa = 3.51ITTTAKK396 pKa = 9.91WSLTGSVLTLHH407 pKa = 6.26YY408 pKa = 10.15RR409 pKa = 11.84FVMSGNKK416 pKa = 6.25TTNGLVTIDD425 pKa = 3.57GLPYY429 pKa = 9.76RR430 pKa = 11.84PEE432 pKa = 4.34GLTHH436 pKa = 6.63HH437 pKa = 6.9AMFITGSTSNSMIGAFTDD455 pKa = 3.57ANYY458 pKa = 11.17SNGNVLRR465 pKa = 11.84PFVYY469 pKa = 10.55LSGGNNPIQLNTGQSLNSGTQILGTVTYY497 pKa = 10.22IINN500 pKa = 3.55

Molecular weight: 52.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A2X0|S0A2X0_9CAUD Uncharacterized protein OS=Cellulophaga phage phi39:1 OX=1327993 GN=Phi39:1_gp45 PE=4 SV=1
MM1 pKa = 7.31TKK3 pKa = 10.6NIILVIVLFSVVSSYY18 pKa = 11.6NLSKK22 pKa = 11.17YY23 pKa = 10.64SDD25 pKa = 5.16GILHH29 pKa = 6.73WKK31 pKa = 10.25SFIPLFFYY39 pKa = 10.99CFLLSVFVVVFFKK52 pKa = 10.89ILVKK56 pKa = 10.41LCKK59 pKa = 10.29SILNGII65 pKa = 4.24

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

9341

33

935

194.6

21.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.905 ± 0.708

0.899 ± 0.18

6.306 ± 0.318

6.263 ± 0.417

4.989 ± 0.278

7.034 ± 0.561

1.092 ± 0.166

7.601 ± 0.317

8.222 ± 0.537

9.175 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.916 ± 0.186

6.188 ± 0.355

2.826 ± 0.177

2.858 ± 0.204

3.287 ± 0.207

7.365 ± 0.417

5.417 ± 0.461

7.034 ± 0.379

1.028 ± 0.113

3.597 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski