Wenling tombus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGT1|A0A1L3KGT1_9VIRU Uncharacterized protein OS=Wenling tombus-like virus 3 OX=1923545 PE=4 SV=1
MM1 pKa = 7.38VILAEE6 pKa = 4.84DD7 pKa = 3.92EE8 pKa = 4.5TKK10 pKa = 10.69SGCGHH15 pKa = 6.03SCRR18 pKa = 11.84TPRR21 pKa = 11.84CTRR24 pKa = 11.84TRR26 pKa = 11.84SGPINGSASCGRR38 pKa = 11.84DD39 pKa = 3.11TSGSRR44 pKa = 11.84HH45 pKa = 5.19SNRR48 pKa = 11.84HH49 pKa = 4.05RR50 pKa = 11.84FRR52 pKa = 11.84YY53 pKa = 8.7RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84GKK59 pKa = 9.24PRR61 pKa = 11.84CPRR64 pKa = 11.84CRR66 pKa = 11.84HH67 pKa = 5.99RR68 pKa = 11.84GDD70 pKa = 3.22GKK72 pKa = 10.75KK73 pKa = 9.89FCVSQRR79 pKa = 11.84CASISATEE87 pKa = 4.14KK88 pKa = 10.58DD89 pKa = 3.56QSILSEE95 pKa = 4.83DD96 pKa = 3.79GMDD99 pKa = 3.25AHH101 pKa = 7.39AGTSCTHH108 pKa = 6.45SGPAKK113 pKa = 9.81TYY115 pKa = 11.1AEE117 pKa = 4.65LSTHH121 pKa = 6.23SSWALPGDD129 pKa = 4.13CGNVAKK135 pKa = 10.53EE136 pKa = 4.04LVQSNSRR143 pKa = 11.84CNIIRR148 pKa = 11.84TPGCGSTTDD157 pKa = 4.22RR158 pKa = 11.84RR159 pKa = 11.84DD160 pKa = 3.45HH161 pKa = 6.84FCGQSGGSDD170 pKa = 3.54VVLKK174 pKa = 10.45AASSVSPSDD183 pKa = 3.42KK184 pKa = 10.49TPLIHH189 pKa = 7.43VSGKK193 pKa = 9.32SEE195 pKa = 3.89EE196 pKa = 4.03VLGRR200 pKa = 11.84FRR202 pKa = 11.84LAASHH207 pKa = 7.07AARR210 pKa = 11.84NLFRR214 pKa = 11.84HH215 pKa = 5.85LTLCCGGIYY224 pKa = 10.28ACRR227 pKa = 11.84EE228 pKa = 4.07RR229 pKa = 11.84VGGAQLDD236 pKa = 4.17DD237 pKa = 3.88SCEE240 pKa = 3.82EE241 pKa = 4.29GLQSVHH247 pKa = 6.37QSRR250 pKa = 4.07

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH11|A0A1L3KH11_9VIRU RNA-directed RNA polymerase OS=Wenling tombus-like virus 3 OX=1923545 PE=4 SV=1
MM1 pKa = 6.91KK2 pKa = 8.97TWSGGICVMPSFHH15 pKa = 6.66TKK17 pKa = 9.2QVGPRR22 pKa = 11.84TNYY25 pKa = 9.51RR26 pKa = 11.84WSFWQRR32 pKa = 11.84MKK34 pKa = 10.89RR35 pKa = 11.84SLDD38 pKa = 2.78VDD40 pKa = 4.17TPVEE44 pKa = 3.87PHH46 pKa = 7.0DD47 pKa = 5.12ALGQGPAPLMGVRR60 pKa = 11.84PVDD63 pKa = 4.84EE64 pKa = 4.94IPVDD68 pKa = 3.63QDD70 pKa = 3.17IPIATASGTVGVEE83 pKa = 4.16VNPGAHH89 pKa = 7.06DD90 pKa = 3.7AAIAATGRR98 pKa = 11.84SFAFHH103 pKa = 6.71NDD105 pKa = 2.03AHH107 pKa = 6.45RR108 pKa = 11.84FLRR111 pKa = 11.84QRR113 pKa = 11.84RR114 pKa = 11.84INRR117 pKa = 11.84FSPKK121 pKa = 9.67TEE123 pKa = 3.55WTRR126 pKa = 11.84MRR128 pKa = 11.84VHH130 pKa = 7.18RR131 pKa = 11.84VPIQDD136 pKa = 3.35QRR138 pKa = 11.84KK139 pKa = 8.38PMPNYY144 pKa = 10.05RR145 pKa = 11.84PTAHH149 pKa = 7.33GLFQEE154 pKa = 4.48TAEE157 pKa = 4.09MLQKK161 pKa = 10.78NWFNPTLDD169 pKa = 3.43VTLSVPRR176 pKa = 11.84GVDD179 pKa = 2.82RR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 11.84IEE184 pKa = 4.56EE185 pKa = 4.03ITSVASPEE193 pKa = 3.93DD194 pKa = 3.58LTWFLRR200 pKa = 11.84RR201 pKa = 11.84QALFRR206 pKa = 11.84PRR208 pKa = 11.84TKK210 pKa = 10.64LLLSMLVEE218 pKa = 4.18KK219 pKa = 10.66AKK221 pKa = 10.88KK222 pKa = 10.13FLDD225 pKa = 4.26DD226 pKa = 3.27SDD228 pKa = 4.98LRR230 pKa = 11.84HH231 pKa = 5.19HH232 pKa = 6.15TQRR235 pKa = 11.84EE236 pKa = 4.36IYY238 pKa = 10.15FGTLHH243 pKa = 6.23SVAAAYY249 pKa = 8.82MPVEE253 pKa = 4.37RR254 pKa = 11.84EE255 pKa = 4.13LEE257 pKa = 4.13VLNWMTAAKK266 pKa = 9.91KK267 pKa = 10.38DD268 pKa = 3.78YY269 pKa = 10.61NQFISPDD276 pKa = 3.75DD277 pKa = 4.11ASSS280 pKa = 2.89

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

998

250

468

332.7

37.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.914 ± 0.443

2.505 ± 1.341

6.112 ± 0.287

4.609 ± 0.136

4.709 ± 0.992

6.513 ± 1.212

3.707 ± 0.37

4.208 ± 0.432

4.509 ± 0.242

8.317 ± 1.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.531

3.607 ± 0.534

5.21 ± 0.807

2.505 ± 0.779

9.82 ± 0.61

8.617 ± 1.467

4.509 ± 1.28

6.212 ± 0.567

1.603 ± 0.401

3.407 ± 1.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski