Brassica cretica (Mustard)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6UTK2|A0A3N6UTK2_BRACR Uncharacterized protein OS=Brassica cretica OX=69181 GN=DY000_00030940 PE=4 SV=1
MM1 pKa = 8.0VSITHH6 pKa = 7.11KK7 pKa = 10.52DD8 pKa = 3.55DD9 pKa = 4.15QIVEE13 pKa = 4.42SKK15 pKa = 10.62EE16 pKa = 3.49IMPISSPKK24 pKa = 9.38NAYY27 pKa = 9.55GSNSDD32 pKa = 4.06EE33 pKa = 5.34LNDD36 pKa = 3.69TDD38 pKa = 3.8EE39 pKa = 4.97EE40 pKa = 4.46EE41 pKa = 5.21VNVCDD46 pKa = 3.48TCGVQGFTNKK56 pKa = 10.03LAICDD61 pKa = 3.74NCGVGAEE68 pKa = 4.23HH69 pKa = 7.04TYY71 pKa = 11.47YY72 pKa = 10.14MAEE75 pKa = 4.11KK76 pKa = 10.7LEE78 pKa = 4.42DD79 pKa = 3.51VPEE82 pKa = 4.02RR83 pKa = 11.84WSCNDD88 pKa = 3.1CVEE91 pKa = 4.27MGEE94 pKa = 5.86SEE96 pKa = 4.21FQKK99 pKa = 10.96QNDD102 pKa = 3.56LSTQKK107 pKa = 10.59SSEE110 pKa = 4.37TVGPDD115 pKa = 4.0LNIEE119 pKa = 4.25PNLNLNPNIDD129 pKa = 4.28LNLDD133 pKa = 3.38PSTDD137 pKa = 3.62LNVNPNLDD145 pKa = 4.18LNLDD149 pKa = 3.81MNLDD153 pKa = 4.17PNLHH157 pKa = 6.44LSVDD161 pKa = 3.77NNLDD165 pKa = 3.4LSLDD169 pKa = 3.79CGSYY173 pKa = 9.36YY174 pKa = 10.47QPSSTSNFQAHH185 pKa = 4.94TRR187 pKa = 11.84MRR189 pKa = 11.84ASPNPLLGPGSLEE202 pKa = 4.1EE203 pKa = 4.33EE204 pKa = 4.37EE205 pKa = 4.94EE206 pKa = 4.28EE207 pKa = 4.48

Molecular weight: 22.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6R561|A0A3N6R561_BRACR Laccase OS=Brassica cretica OX=69181 GN=DY000_00018611 PE=3 SV=1
MM1 pKa = 7.53AANGVRR7 pKa = 11.84RR8 pKa = 11.84ALHH11 pKa = 6.11SSSSTGRR18 pKa = 11.84ILFSRR23 pKa = 11.84SSSVPKK29 pKa = 10.13LGKK32 pKa = 9.73SAGFAFGNGSSSSRR46 pKa = 11.84SSLRR50 pKa = 11.84RR51 pKa = 11.84LTSSS55 pKa = 2.95

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30345

0

30345

9102698

49

5306

300.0

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.479 ± 0.016

1.838 ± 0.007

5.381 ± 0.011

6.805 ± 0.023

4.116 ± 0.009

6.402 ± 0.015

2.346 ± 0.007

4.969 ± 0.01

6.03 ± 0.017

9.489 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.006

4.111 ± 0.01

5.025 ± 0.017

3.502 ± 0.009

5.908 ± 0.015

9.274 ± 0.016

5.225 ± 0.011

6.699 ± 0.012

1.285 ± 0.005

2.64 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski