Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobium/Pelodictyon group; Pelodictyon; Pelodictyon phaeoclathratiforme

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2685 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4SE54|B4SE54_PELPB DUF4325 domain-containing protein OS=Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) OX=324925 GN=Ppha_2278 PE=4 SV=1
MM1 pKa = 6.54FTGVIGDD8 pKa = 3.73YY9 pKa = 10.73TITTLEE15 pKa = 4.69DD16 pKa = 3.54GSGLRR21 pKa = 11.84VEE23 pKa = 5.55DD24 pKa = 4.85KK25 pKa = 10.47ISNRR29 pKa = 11.84DD30 pKa = 3.4GVDD33 pKa = 2.7ILYY36 pKa = 8.68DD37 pKa = 3.63TEE39 pKa = 4.01FLRR42 pKa = 11.84FEE44 pKa = 4.63GSPGTADD51 pKa = 3.36TPTVQPEE58 pKa = 3.61AGIYY62 pKa = 8.83WSARR66 pKa = 11.84DD67 pKa = 4.25IILGTANHH75 pKa = 6.84HH76 pKa = 5.96FLEE79 pKa = 5.01ILPSWPEE86 pKa = 3.71TFDD89 pKa = 3.57FNRR92 pKa = 11.84DD93 pKa = 3.24GTIDD97 pKa = 3.72TVDD100 pKa = 3.73LDD102 pKa = 4.01GDD104 pKa = 4.01GVRR107 pKa = 11.84DD108 pKa = 3.66GFTIGGTEE116 pKa = 3.88EE117 pKa = 4.0SGKK120 pKa = 10.49LVAYY124 pKa = 10.67LNDD127 pKa = 3.85AADD130 pKa = 3.29IAAILGSNEE139 pKa = 3.76EE140 pKa = 4.1SHH142 pKa = 7.4LIPLPAGVSYY152 pKa = 11.39DD153 pKa = 3.71EE154 pKa = 5.87LIAALYY160 pKa = 7.69TAYY163 pKa = 9.84TNYY166 pKa = 10.5LDD168 pKa = 4.28EE169 pKa = 4.8SVDD172 pKa = 3.67YY173 pKa = 10.22TLLPTADD180 pKa = 4.95ADD182 pKa = 4.01NLDD185 pKa = 4.32NGNCATWVNTILQVVGITNTAGLGDD210 pKa = 4.14FSGIDD215 pKa = 3.42AGDD218 pKa = 3.54TSTLAFSYY226 pKa = 7.48FTT228 pKa = 4.28

Molecular weight: 24.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4SBT3|B4SBT3_PELPB TIR protein OS=Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) OX=324925 GN=Ppha_0273 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.94ALGQRR7 pKa = 11.84LRR9 pKa = 11.84DD10 pKa = 3.24ARR12 pKa = 11.84LRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84FSMEE20 pKa = 3.93TVCARR25 pKa = 11.84ANLSRR30 pKa = 11.84PTLYY34 pKa = 10.49KK35 pKa = 10.1IEE37 pKa = 4.38TGDD40 pKa = 3.46PSVAIGFYY48 pKa = 9.58VQVLRR53 pKa = 11.84VLGLLADD60 pKa = 4.6LSLISKK66 pKa = 9.97EE67 pKa = 4.03DD68 pKa = 3.37ALGRR72 pKa = 11.84RR73 pKa = 11.84LQDD76 pKa = 2.71EE77 pKa = 4.31SLPRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84APRR87 pKa = 11.84KK88 pKa = 9.01KK89 pKa = 10.36APAGASDD96 pKa = 4.74GEE98 pKa = 4.45PGSCAIFLPQPP109 pKa = 3.54

Molecular weight: 12.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2685

0

2685

851977

30

7233

317.3

35.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.387 ± 0.058

1.126 ± 0.02

5.172 ± 0.053

6.854 ± 0.055

4.443 ± 0.039

7.026 ± 0.051

2.175 ± 0.025

6.727 ± 0.041

5.595 ± 0.054

10.451 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.025

3.842 ± 0.038

4.136 ± 0.029

3.467 ± 0.028

5.474 ± 0.055

6.611 ± 0.05

5.224 ± 0.091

6.641 ± 0.05

1.093 ± 0.02

3.076 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski