Reovirus GCRV104

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H9XH60|H9XH60_9REOV Uncharacterized protein OS=Reovirus GCRV104 OX=1164802 PE=4 SV=1
MM1 pKa = 7.58GNAQTSVTNVTVNGDD16 pKa = 3.67GNVIRR21 pKa = 11.84PSAEE25 pKa = 3.84FQATAAPSFDD35 pKa = 4.76LNPGVLNPNGNLYY48 pKa = 10.0IEE50 pKa = 4.47QPDD53 pKa = 3.18MDD55 pKa = 4.22ATVTAQSLVLLHH67 pKa = 7.35DD68 pKa = 4.1KK69 pKa = 10.36TGSPYY74 pKa = 10.91AYY76 pKa = 10.11VADD79 pKa = 4.11NTRR82 pKa = 11.84EE83 pKa = 4.05TLDD86 pKa = 4.08KK87 pKa = 10.42IVNYY91 pKa = 9.58IANFQPLLEE100 pKa = 4.72ASGLTCPPRR109 pKa = 11.84MISFYY114 pKa = 9.61CEE116 pKa = 3.47RR117 pKa = 11.84MSGYY121 pKa = 10.64VGTSAITAINNPVEE135 pKa = 4.67DD136 pKa = 4.67GEE138 pKa = 5.23FITQAKK144 pKa = 7.69MAQFRR149 pKa = 11.84KK150 pKa = 9.82QLGLFYY156 pKa = 11.02DD157 pKa = 4.2KK158 pKa = 11.46VKK160 pKa = 10.63DD161 pKa = 3.28WHH163 pKa = 7.01VDD165 pKa = 3.26LKK167 pKa = 10.26MAQAMLPLNVPTGTYY182 pKa = 8.78QLEE185 pKa = 4.24FTSYY189 pKa = 10.21RR190 pKa = 11.84DD191 pKa = 3.44FMTEE195 pKa = 3.87YY196 pKa = 10.4LPEE199 pKa = 4.45SNICKK204 pKa = 10.24LYY206 pKa = 9.86PDD208 pKa = 4.03EE209 pKa = 4.56ALEE212 pKa = 4.2VAANLIPAIRR222 pKa = 11.84TGLTKK227 pKa = 10.39EE228 pKa = 3.78ISDD231 pKa = 3.95VNSTGLIAGAIMAPQEE247 pKa = 4.52GGGSLTDD254 pKa = 3.89GSNTGTSLLQDD265 pKa = 3.81VASMNANVITASSTIPVSSLNVIPEE290 pKa = 4.29PKK292 pKa = 10.06SFSYY296 pKa = 11.02DD297 pKa = 2.9SLAPDD302 pKa = 3.31EE303 pKa = 5.11VYY305 pKa = 10.21WIQQVPSVQVRR316 pKa = 11.84FSVDD320 pKa = 3.11VEE322 pKa = 4.33TVTYY326 pKa = 11.11YY327 pKa = 10.24MGFEE331 pKa = 4.03PAQVNTVIDD340 pKa = 3.91LRR342 pKa = 11.84EE343 pKa = 4.12CSSASFVFDD352 pKa = 3.2LTGTQFAADD361 pKa = 4.06YY362 pKa = 7.19PTSKK366 pKa = 10.6LGLLMIRR373 pKa = 11.84LTDD376 pKa = 3.93SITAVTPSTEE386 pKa = 3.28IKK388 pKa = 10.94ANVLEE393 pKa = 4.77ISDD396 pKa = 3.54ATIQQRR402 pKa = 11.84SRR404 pKa = 11.84KK405 pKa = 8.6AVSGLSQLATTVLTYY420 pKa = 9.89TYY422 pKa = 10.18EE423 pKa = 4.65RR424 pKa = 11.84EE425 pKa = 4.1VLPNDD430 pKa = 3.37DD431 pKa = 3.51AYY433 pKa = 11.54YY434 pKa = 10.75YY435 pKa = 10.29LVPCTMQPTAYY446 pKa = 8.31TALNYY451 pKa = 8.49PCSVGGTLTVTPIDD465 pKa = 3.73NTEE468 pKa = 3.91VTVNGIMVDD477 pKa = 3.76SIIPSEE483 pKa = 4.59LIGSQTEE490 pKa = 4.24EE491 pKa = 3.9EE492 pKa = 4.93QMTCLPNEE500 pKa = 3.86AAEE503 pKa = 4.08LLKK506 pKa = 10.97HH507 pKa = 6.0KK508 pKa = 10.48VQICAGTIAKK518 pKa = 8.36MDD520 pKa = 4.05PSGVSEE526 pKa = 4.5DD527 pKa = 3.99TPAVAAVAGILALQNEE543 pKa = 4.52TRR545 pKa = 11.84AVRR548 pKa = 11.84LDD550 pKa = 3.23IKK552 pKa = 11.09SLFSKK557 pKa = 10.43ARR559 pKa = 11.84RR560 pKa = 11.84AINMLVGRR568 pKa = 11.84PSTVLGLAKK577 pKa = 10.08PVISDD582 pKa = 3.72PNVWMQIATGVAEE595 pKa = 4.73AVASGNVNSAMRR607 pKa = 11.84DD608 pKa = 3.37SARR611 pKa = 11.84RR612 pKa = 11.84LAALRR617 pKa = 11.84SVSGAKK623 pKa = 9.96GKK625 pKa = 10.11LLSLVAQRR633 pKa = 11.84FPPNPP638 pKa = 3.43

Molecular weight:
68.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H9XH56|H9XH56_9REOV VP3 OS=Reovirus GCRV104 OX=1164802 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 3.92NHH4 pKa = 7.44DD5 pKa = 4.26DD6 pKa = 3.89LAVEE10 pKa = 4.26RR11 pKa = 11.84LINCLTGEE19 pKa = 4.86LEE21 pKa = 4.14LTDD24 pKa = 6.48DD25 pKa = 4.26IFLAANSALRR35 pKa = 11.84SRR37 pKa = 11.84DD38 pKa = 2.74RR39 pKa = 11.84SYY41 pKa = 11.77VYY43 pKa = 10.28RR44 pKa = 11.84QLNAIPFHH52 pKa = 5.85TPVYY56 pKa = 7.53VTRR59 pKa = 11.84AILVNRR65 pKa = 11.84DD66 pKa = 2.69HH67 pKa = 7.57RR68 pKa = 11.84DD69 pKa = 3.35YY70 pKa = 11.76YY71 pKa = 11.11HH72 pKa = 6.8IDD74 pKa = 2.89SRR76 pKa = 11.84GLVRR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 10.39LSVTVGADD90 pKa = 3.1DD91 pKa = 4.62DD92 pKa = 5.66VYY94 pKa = 11.65VPCCNIGPLLAPHH107 pKa = 6.52RR108 pKa = 11.84TLEE111 pKa = 4.57GYY113 pKa = 10.66GILDD117 pKa = 3.63PKK119 pKa = 10.48FAATIKK125 pKa = 10.83DD126 pKa = 3.5PASRR130 pKa = 11.84IAITFAKK137 pKa = 10.03SATVQARR144 pKa = 11.84QVKK147 pKa = 10.42DD148 pKa = 3.87DD149 pKa = 3.33ITRR152 pKa = 11.84FLQALLLMNGKK163 pKa = 8.61QAIVGDD169 pKa = 4.58EE170 pKa = 4.09YY171 pKa = 11.66ADD173 pKa = 4.12RR174 pKa = 11.84SPTPPCEE181 pKa = 4.03TCPALHH187 pKa = 6.69AVSQVIQLLGFAPPNLSPFLALPAGGLWHH216 pKa = 6.58MPGTAKK222 pKa = 10.3VMPVQVPQLLNDD234 pKa = 4.5LVNLAILRR242 pKa = 11.84VMLRR246 pKa = 11.84IPDD249 pKa = 3.56EE250 pKa = 3.82LTYY253 pKa = 10.85FGVRR257 pKa = 11.84VLLAAAYY264 pKa = 9.9SEE266 pKa = 4.61SYY268 pKa = 10.01SWAVLQCKK276 pKa = 9.97HH277 pKa = 6.33FFPDD281 pKa = 3.98LSVHH285 pKa = 6.89AMYY288 pKa = 8.45KK289 pKa = 9.18TNFSGGYY296 pKa = 10.03APIFDD301 pKa = 4.19WPSPRR306 pKa = 11.84SDD308 pKa = 3.27YY309 pKa = 11.09RR310 pKa = 11.84FTYY313 pKa = 10.02LGHH316 pKa = 6.27RR317 pKa = 11.84TMTSEE322 pKa = 4.7DD323 pKa = 4.06KK324 pKa = 10.81PVLPLDD330 pKa = 3.79LKK332 pKa = 11.15KK333 pKa = 10.64VDD335 pKa = 3.22EE336 pKa = 4.4TARR339 pKa = 11.84KK340 pKa = 9.13FDD342 pKa = 3.88LVDD345 pKa = 3.02VAEE348 pKa = 4.16RR349 pKa = 11.84MKK351 pKa = 10.72TYY353 pKa = 10.47LADD356 pKa = 3.83INTHH360 pKa = 4.57THH362 pKa = 6.05ASMKK366 pKa = 9.43YY367 pKa = 8.07AHH369 pKa = 6.95HH370 pKa = 6.68VMALTSSSFTVQTATEE386 pKa = 4.21SVYY389 pKa = 11.18AEE391 pKa = 4.03YY392 pKa = 9.17TQNPVIEE399 pKa = 4.31QPIPPDD405 pKa = 2.96SWTGPIGYY413 pKa = 9.28LRR415 pKa = 11.84LLKK418 pKa = 9.9TDD420 pKa = 3.69APAPALSLHH429 pKa = 5.03RR430 pKa = 11.84TWRR433 pKa = 11.84NAAVAVAKK441 pKa = 10.46DD442 pKa = 3.8MRR444 pKa = 11.84QMDD447 pKa = 4.72PLQQAIMRR455 pKa = 11.84TQYY458 pKa = 8.06VTPRR462 pKa = 11.84GGSGYY467 pKa = 8.58SLRR470 pKa = 11.84TSLEE474 pKa = 3.77AANVAIRR481 pKa = 11.84DD482 pKa = 3.72FRR484 pKa = 11.84NAGVKK489 pKa = 9.94ISTKK493 pKa = 9.85IYY495 pKa = 10.13QAAQTAQIPFEE506 pKa = 4.18VLLPAVLAAVTLGTRR521 pKa = 11.84FQVQRR526 pKa = 11.84RR527 pKa = 11.84PRR529 pKa = 11.84AIMPLNIVQQTVSAIHH545 pKa = 6.43TIVADD550 pKa = 4.06YY551 pKa = 10.82INKK554 pKa = 9.24HH555 pKa = 5.61MNLSTTSGSAVQEE568 pKa = 3.98KK569 pKa = 10.32VIPLVLFASTTPTTVVNVDD588 pKa = 3.13VKK590 pKa = 11.4ACDD593 pKa = 3.21ACVTYY598 pKa = 10.55SYY600 pKa = 10.56FLSVICAAMYY610 pKa = 9.84EE611 pKa = 4.37GLNPHH616 pKa = 6.83GDD618 pKa = 3.6PRR620 pKa = 11.84PFMGVPVLPYY630 pKa = 8.95TNRR633 pKa = 11.84VSSAMMTDD641 pKa = 3.64EE642 pKa = 4.36ASGMQVMVQQLARR655 pKa = 11.84LYY657 pKa = 10.14QNGFRR662 pKa = 11.84YY663 pKa = 8.75MVSDD667 pKa = 3.79PMAPGNIFEE676 pKa = 5.38LPTLTFPSGSTATSTEE692 pKa = 3.82HH693 pKa = 5.93TANNSTMMDD702 pKa = 3.43HH703 pKa = 7.83FITTWLPEE711 pKa = 3.92HH712 pKa = 5.72TTNPEE717 pKa = 3.25LKK719 pKa = 10.48AIVRR723 pKa = 11.84QMSIRR728 pKa = 11.84DD729 pKa = 3.62NYY731 pKa = 9.3VCQGDD736 pKa = 4.29DD737 pKa = 3.7GMCIIDD743 pKa = 3.74NVSGRR748 pKa = 11.84RR749 pKa = 11.84ISAEE753 pKa = 3.77SLTEE757 pKa = 4.02FTTLLNKK764 pKa = 10.29YY765 pKa = 8.54GRR767 pKa = 11.84QFGWVYY773 pKa = 10.78DD774 pKa = 3.88IDD776 pKa = 4.28ASGCAEE782 pKa = 4.03YY783 pKa = 11.05LKK785 pKa = 10.32IYY787 pKa = 9.85AVNGCRR793 pKa = 11.84VPNCSRR799 pKa = 11.84HH800 pKa = 5.82PILGKK805 pKa = 10.68EE806 pKa = 4.13MANPDD811 pKa = 4.73AYY813 pKa = 10.79DD814 pKa = 3.64PWPQIINALMGLWRR828 pKa = 11.84SGLTDD833 pKa = 4.36AYY835 pKa = 10.51DD836 pKa = 3.7TVDD839 pKa = 2.71WTRR842 pKa = 11.84YY843 pKa = 5.66MWALVTIYY851 pKa = 10.77ASGSMTTTKK860 pKa = 10.52GQRR863 pKa = 11.84LRR865 pKa = 11.84LAYY868 pKa = 9.74PMSTFITLGIPPIRR882 pKa = 11.84IWGCDD887 pKa = 3.2PFTVSVYY894 pKa = 9.64MPPGDD899 pKa = 3.43MGVYY903 pKa = 10.69ALINVLRR910 pKa = 11.84HH911 pKa = 5.74HH912 pKa = 7.63LYY914 pKa = 8.02TTIAAHH920 pKa = 6.01GDD922 pKa = 3.2KK923 pKa = 10.56YY924 pKa = 10.98RR925 pKa = 11.84QDD927 pKa = 3.77RR928 pKa = 11.84GDD930 pKa = 4.22DD931 pKa = 3.36MFDD934 pKa = 3.13GHH936 pKa = 6.68NVLQFLHH943 pKa = 6.48DD944 pKa = 3.42TRR946 pKa = 11.84FTHH949 pKa = 7.39GYY951 pKa = 8.47FAAAHH956 pKa = 5.87HH957 pKa = 6.01RR958 pKa = 11.84VPLRR962 pKa = 11.84EE963 pKa = 3.99SRR965 pKa = 11.84EE966 pKa = 3.76VDD968 pKa = 2.9AKK970 pKa = 11.21ARR972 pKa = 11.84LDD974 pKa = 3.88MISALSDD981 pKa = 3.36FLYY984 pKa = 10.56HH985 pKa = 7.56DD986 pKa = 3.87PEE988 pKa = 4.13LRR990 pKa = 11.84FRR992 pKa = 11.84VIAGRR997 pKa = 11.84RR998 pKa = 11.84LWDD1001 pKa = 3.35TYY1003 pKa = 11.63GQGQHH1008 pKa = 5.68GAYY1011 pKa = 9.67LHH1013 pKa = 6.49RR1014 pKa = 11.84VPSLDD1019 pKa = 3.64DD1020 pKa = 3.57VPHH1023 pKa = 6.35RR1024 pKa = 11.84WYY1026 pKa = 10.62EE1027 pKa = 3.84EE1028 pKa = 3.48ARR1030 pKa = 11.84EE1031 pKa = 3.95ADD1033 pKa = 3.85RR1034 pKa = 11.84ATLSDD1039 pKa = 3.71EE1040 pKa = 3.8EE1041 pKa = 4.6RR1042 pKa = 11.84LFKK1045 pKa = 10.7SLHH1048 pKa = 5.51RR1049 pKa = 11.84AITRR1053 pKa = 11.84PSLMLSKK1060 pKa = 11.06LLMAYY1065 pKa = 10.22LRR1067 pKa = 11.84VRR1069 pKa = 11.84WDD1071 pKa = 2.87IGEE1074 pKa = 4.38LMPAAVSPQVPLTAGMHH1091 pKa = 5.46PQNPDD1096 pKa = 2.52MFMKK1100 pKa = 8.25MTSLGPFLEE1109 pKa = 4.4RR1110 pKa = 11.84VGQYY1114 pKa = 9.69FHH1116 pKa = 7.8DD1117 pKa = 3.94ALFVKK1122 pKa = 10.21RR1123 pKa = 11.84VVSGMDD1129 pKa = 3.39VNTLDD1134 pKa = 4.34AALLKK1139 pKa = 10.69LRR1141 pKa = 11.84ILGAPANALVGVIMQSGFSEE1161 pKa = 4.44SEE1163 pKa = 3.72AAEE1166 pKa = 3.76IAGRR1170 pKa = 11.84IQLKK1174 pKa = 9.73DD1175 pKa = 3.15ARR1177 pKa = 11.84TVQIARR1183 pKa = 11.84VVNLSVPDD1191 pKa = 3.17TWMLFNFHH1199 pKa = 7.05LLLHH1203 pKa = 6.1ATVNPHH1209 pKa = 5.61GMYY1212 pKa = 10.67GQTTFTKK1219 pKa = 9.97IPPSMPWLRR1228 pKa = 11.84SILLFLGSSINMTRR1242 pKa = 11.84VGPIHH1247 pKa = 5.87QVYY1250 pKa = 9.62LARR1253 pKa = 11.84IHH1255 pKa = 5.84GTGASLARR1263 pKa = 11.84QFQQWMSSAHH1273 pKa = 6.04KK1274 pKa = 10.49

Molecular weight:
142.6 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7523

140

1294

683.9

75.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.305 ± 0.299

1.196 ± 0.16

5.516 ± 0.14

3.722 ± 0.282

3.27 ± 0.265

5.37 ± 0.326

2.353 ± 0.334

5.251 ± 0.288

2.459 ± 0.353

9.451 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.523 ± 0.176

4.161 ± 0.369

6.886 ± 0.352

4.373 ± 0.215

5.543 ± 0.304

7.298 ± 0.529

7.563 ± 0.264

7.922 ± 0.196

1.276 ± 0.141

3.562 ± 0.308

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski