Rhodopila globiformis (Rhodopseudomonas globiformis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Rhodopila

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6MV27|A0A2S6MV27_RHOGL Oxygen-dependent coproporphyrinogen-III oxidase OS=Rhodopila globiformis OX=1071 GN=hemF PE=3 SV=1
MM1 pKa = 7.42SGVGSVGSGYY11 pKa = 11.04SFLNTLVANALDD23 pKa = 3.54VHH25 pKa = 5.77QQLNTLTEE33 pKa = 3.97QASTGLVAQTYY44 pKa = 10.64AEE46 pKa = 4.6LGSGASVALDD56 pKa = 4.57LNPQLATLQTYY67 pKa = 8.93QNNISQATGRR77 pKa = 11.84MQVTQTAMTQLQQIAATFVAAMPNLNSTSSQEE109 pKa = 3.75VDD111 pKa = 3.66TIAAQARR118 pKa = 11.84DD119 pKa = 3.69ALVQVANLLDD129 pKa = 3.93TQDD132 pKa = 2.99GGVYY136 pKa = 10.31VFGGQDD142 pKa = 3.09TANPPVPNPDD152 pKa = 4.12SILTSTSPDD161 pKa = 3.27FYY163 pKa = 11.1GTINQTVSDD172 pKa = 4.12YY173 pKa = 11.14LNNVSGVTLSTTVTDD188 pKa = 5.86AIANAPFSANMQSGTVGQQVVQTQDD213 pKa = 2.9GSSVRR218 pKa = 11.84IGLLANQNASAATTSTPTGSYY239 pKa = 9.61MLDD242 pKa = 3.3LMRR245 pKa = 11.84SLAIVGSLSSTQTGDD260 pKa = 3.55SNFMPLVQDD269 pKa = 4.12TMTTLNGVVSAMATDD284 pKa = 3.64VGVLGNRR291 pKa = 11.84QSALTATQTQLSDD304 pKa = 2.91TATALTGQVSAVQDD318 pKa = 3.37ADD320 pKa = 3.59MTTTLSKK327 pKa = 9.93LTAVQTQLQMSYY339 pKa = 10.85RR340 pKa = 11.84MIVSEE345 pKa = 4.39GTLSLSTYY353 pKa = 10.57LPVAA357 pKa = 4.16

Molecular weight: 36.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6MU69|A0A2S6MU69_RHOGL Glutamate synthase large subunit OS=Rhodopila globiformis OX=1071 GN=CCS01_31570 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.9LVRR12 pKa = 11.84KK13 pKa = 8.84HH14 pKa = 5.05RR15 pKa = 11.84HH16 pKa = 3.93GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.09VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.85GRR39 pKa = 11.84KK40 pKa = 8.87RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6183

0

6183

1919277

29

1767

310.4

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.551 ± 0.052

0.997 ± 0.011

5.559 ± 0.021

4.908 ± 0.029

3.354 ± 0.018

8.667 ± 0.028

2.27 ± 0.015

4.658 ± 0.024

2.388 ± 0.022

10.363 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.014

2.396 ± 0.017

5.946 ± 0.028

3.36 ± 0.021

7.823 ± 0.032

4.848 ± 0.023

5.395 ± 0.025

7.5 ± 0.025

1.482 ± 0.014

2.083 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski