Escherichia phage LAMP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; unclassified Kuravirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2I6PD12|A0A2I6PD12_9CAUD Uncharacterized protein OS=Escherichia phage LAMP OX=2065191 GN=phiL_039 PE=4 SV=1
MM1 pKa = 7.42INKK4 pKa = 9.58IKK6 pKa = 10.86DD7 pKa = 3.45NEE9 pKa = 4.13YY10 pKa = 10.85LLNSSILDD18 pKa = 3.53NGNTIDD24 pKa = 6.29FITLSEE30 pKa = 4.15LKK32 pKa = 10.55EE33 pKa = 4.19LVNSLYY39 pKa = 10.81SEE41 pKa = 4.11EE42 pKa = 4.1QNRR45 pKa = 11.84LLQEE49 pKa = 4.31SLSGDD54 pKa = 4.09YY55 pKa = 11.19VCDD58 pKa = 3.48GCLII62 pKa = 4.04

Molecular weight:
7.04 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2I6PD32|A0A2I6PD32_9CAUD Uncharacterized protein OS=Escherichia phage LAMP OX=2065191 GN=phiL_070 PE=4 SV=1
MM1 pKa = 7.44SRR3 pKa = 11.84LHH5 pKa = 5.61STKK8 pKa = 10.26KK9 pKa = 10.3NNFYY13 pKa = 9.99RR14 pKa = 11.84QRR16 pKa = 11.84RR17 pKa = 11.84ARR19 pKa = 11.84SNASRR24 pKa = 11.84KK25 pKa = 8.17MKK27 pKa = 10.13GMQLTYY33 pKa = 10.77EE34 pKa = 4.14QIKK37 pKa = 9.94QINNIISQVVWEE49 pKa = 4.43VTVGEE54 pKa = 4.44PEE56 pKa = 3.65

Molecular weight:
6.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

20410

38

1450

214.8

24.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.143 ± 0.534

1.068 ± 0.148

6.164 ± 0.28

7.075 ± 0.381

3.797 ± 0.158

7.134 ± 0.357

1.671 ± 0.145

5.806 ± 0.206

6.002 ± 0.338

7.202 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.685 ± 0.189

5.884 ± 0.253

3.704 ± 0.165

4.429 ± 0.318

5.365 ± 0.274

6.786 ± 0.231

5.698 ± 0.311

6.585 ± 0.28

1.372 ± 0.117

3.43 ± 0.167

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski