Changjiang tombus-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFW7|A0A1L3KFW7_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 15 OX=1922808 PE=4 SV=1
MM1 pKa = 7.52PPGVEE6 pKa = 4.11CHH8 pKa = 6.92SLGLDD13 pKa = 2.83MKK15 pKa = 10.49GRR17 pKa = 11.84HH18 pKa = 5.05KK19 pKa = 10.61EE20 pKa = 3.7RR21 pKa = 11.84YY22 pKa = 9.3LVMDD26 pKa = 4.07TTRR29 pKa = 11.84PAAGLCFTHH38 pKa = 6.54NNSVPNILRR47 pKa = 11.84GLGEE51 pKa = 4.49RR52 pKa = 11.84LMMVPNDD59 pKa = 4.42DD60 pKa = 4.9GGFSPPPEE68 pKa = 3.88PTVFDD73 pKa = 3.82LGEE76 pKa = 3.85YY77 pKa = 10.2SKK79 pKa = 11.53AVLRR83 pKa = 11.84KK84 pKa = 8.45MPKK87 pKa = 8.94HH88 pKa = 6.06LEE90 pKa = 4.09PLEE93 pKa = 4.49HH94 pKa = 7.51DD95 pKa = 4.29EE96 pKa = 6.18FVMLYY101 pKa = 10.22DD102 pKa = 3.55GPKK105 pKa = 8.68RR106 pKa = 11.84KK107 pKa = 9.55RR108 pKa = 11.84YY109 pKa = 8.15EE110 pKa = 3.67AAARR114 pKa = 11.84ALLEE118 pKa = 4.36RR119 pKa = 11.84EE120 pKa = 4.46LDD122 pKa = 3.71AKK124 pKa = 10.65DD125 pKa = 3.29WQIKK129 pKa = 10.27LFIKK133 pKa = 10.7DD134 pKa = 4.13EE135 pKa = 4.5IVCSWAKK142 pKa = 10.65ADD144 pKa = 3.82PAPRR148 pKa = 11.84LISPRR153 pKa = 11.84SPEE156 pKa = 3.63YY157 pKa = 10.35CLEE160 pKa = 4.07VGCFIKK166 pKa = 10.59PIEE169 pKa = 4.33HH170 pKa = 7.15LLYY173 pKa = 10.69KK174 pKa = 10.52AVARR178 pKa = 11.84VWGEE182 pKa = 3.6TTIAKK187 pKa = 9.81GLNFNQRR194 pKa = 11.84GEE196 pKa = 4.23LIKK199 pKa = 10.68EE200 pKa = 3.8KK201 pKa = 10.06WNSFKK206 pKa = 11.16KK207 pKa = 9.99PVAVGLDD214 pKa = 3.18ASRR217 pKa = 11.84FDD219 pKa = 3.48QHH221 pKa = 7.45VSLSALQWEE230 pKa = 4.26HH231 pKa = 7.23SIYY234 pKa = 10.67LGCFPKK240 pKa = 10.24HH241 pKa = 5.85RR242 pKa = 11.84RR243 pKa = 11.84RR244 pKa = 11.84LQKK247 pKa = 10.69LLSKK251 pKa = 9.19QLRR254 pKa = 11.84NFGVCYY260 pKa = 10.58VDD262 pKa = 3.69DD263 pKa = 3.93HH264 pKa = 7.07RR265 pKa = 11.84ITYY268 pKa = 10.27SRR270 pKa = 11.84DD271 pKa = 2.97GGRR274 pKa = 11.84MSGDD278 pKa = 3.19MNTALGNCLIMTGLVWTYY296 pKa = 10.68AKK298 pKa = 10.26QRR300 pKa = 11.84GITVKK305 pKa = 10.73LINDD309 pKa = 3.58GDD311 pKa = 4.07DD312 pKa = 3.15CVVFMEE318 pKa = 4.96EE319 pKa = 3.74SDD321 pKa = 4.31LPAFMEE327 pKa = 5.24GLRR330 pKa = 11.84QWFLEE335 pKa = 3.79RR336 pKa = 11.84GFNMKK341 pKa = 9.7VEE343 pKa = 4.29QPAYY347 pKa = 8.68EE348 pKa = 4.05LEE350 pKa = 5.08AIEE353 pKa = 5.26FCQCHH358 pKa = 5.65PVFNGDD364 pKa = 3.2QYY366 pKa = 10.31TMCRR370 pKa = 11.84NIHH373 pKa = 6.18KK374 pKa = 10.72ALFTDD379 pKa = 4.21VAHH382 pKa = 6.75VGRR385 pKa = 11.84TAEE388 pKa = 4.27EE389 pKa = 3.57IRR391 pKa = 11.84GIRR394 pKa = 11.84EE395 pKa = 3.71AVALCGAAWSKK406 pKa = 10.65GLPIFPTFYY415 pKa = 10.2AGLRR419 pKa = 11.84TGHH422 pKa = 6.38KK423 pKa = 9.88PSVLRR428 pKa = 11.84HH429 pKa = 6.19SGTFWNSQGCKK440 pKa = 10.09SGTKK444 pKa = 9.97YY445 pKa = 8.46VTPRR449 pKa = 11.84ARR451 pKa = 11.84LSFEE455 pKa = 3.89RR456 pKa = 11.84AFGLSPSEE464 pKa = 3.71QVAIEE469 pKa = 4.22NFYY472 pKa = 11.17QSLPRR477 pKa = 11.84TSIDD481 pKa = 3.33EE482 pKa = 3.82PHH484 pKa = 6.6LVFEE488 pKa = 5.1HH489 pKa = 6.74SPTDD493 pKa = 3.44SSTHH497 pKa = 5.87HH498 pKa = 7.01PLFVSDD504 pKa = 3.89SLNYY508 pKa = 10.87LLFNHH513 pKa = 6.72GKK515 pKa = 9.99EE516 pKa = 4.23EE517 pKa = 4.0

Molecular weight: 58.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFW7|A0A1L3KFW7_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 15 OX=1922808 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 9.98KK4 pKa = 10.29SNKK7 pKa = 9.39SPQVQKK13 pKa = 6.72TTRR16 pKa = 11.84PKK18 pKa = 9.0RR19 pKa = 11.84QRR21 pKa = 11.84KK22 pKa = 8.41GRR24 pKa = 11.84IAGVQGKK31 pKa = 9.12ALQLARR37 pKa = 11.84LLNDD41 pKa = 3.58PCSAEE46 pKa = 3.99IPAGSIYY53 pKa = 10.67SGEE56 pKa = 4.07TGIVSRR62 pKa = 11.84FAVEE66 pKa = 4.5LGAGSAAGEE75 pKa = 4.22TCGAILFHH83 pKa = 7.09PNDD86 pKa = 3.18NTLAVFTQANPATTFVVNAGNFVNTNGPGQLFLQSNAAKK125 pKa = 9.21MRR127 pKa = 11.84SLAACITAMPTSSTLNCTGDD147 pKa = 3.57VAVGNITLSSVYY159 pKa = 10.39GATVSINSIFSLLTVRR175 pKa = 11.84GPMVRR180 pKa = 11.84KK181 pKa = 9.95NYY183 pKa = 7.54EE184 pKa = 4.0CKK186 pKa = 9.56MVPGAFDD193 pKa = 2.98SRR195 pKa = 11.84YY196 pKa = 10.16AKK198 pKa = 10.5VIAAGNPSTTGTDD211 pKa = 3.52DD212 pKa = 3.66SDD214 pKa = 3.71TSVICLAFRR223 pKa = 11.84GLPAASGLLYY233 pKa = 10.73RR234 pKa = 11.84LTSVVEE240 pKa = 3.59WTPILNVGLTATTTSNVGIKK260 pKa = 10.08HH261 pKa = 5.28EE262 pKa = 4.26QVVSALSKK270 pKa = 10.64SHH272 pKa = 6.29GGWWHH277 pKa = 6.05NLWNNIGSDD286 pKa = 3.47LGGVASHH293 pKa = 6.75LTSQVLTGGAKK304 pKa = 10.26AIAGLLLL311 pKa = 3.99

Molecular weight: 32.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

828

311

517

414.0

45.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.213 ± 1.717

2.415 ± 0.274

4.106 ± 0.866

5.314 ± 1.729

4.348 ± 0.821

8.454 ± 1.036

3.019 ± 0.797

4.348 ± 0.268

5.314 ± 0.459

9.662 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.388

4.589 ± 1.04

5.314 ± 0.64

2.899 ± 0.179

5.676 ± 1.026

7.246 ± 1.173

6.039 ± 1.674

6.643 ± 0.788

1.57 ± 0.16

2.536 ± 0.524
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski