Hubei tombus-like virus 36

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGD7|A0A1L3KGD7_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 36 OX=1923284 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84SGVRR6 pKa = 11.84KK7 pKa = 9.93RR8 pKa = 11.84FAIKK12 pKa = 9.69TPTPKK17 pKa = 9.93PGKK20 pKa = 9.67LDD22 pKa = 3.49RR23 pKa = 11.84LGEE26 pKa = 4.26FVDD29 pKa = 5.03KK30 pKa = 10.45WLQQNLTPLAPDD42 pKa = 3.1SDD44 pKa = 4.15TTVEE48 pKa = 3.93HH49 pKa = 6.85WLSQTNYY56 pKa = 7.67PAYY59 pKa = 10.3RR60 pKa = 11.84RR61 pKa = 11.84AEE63 pKa = 4.03LLDD66 pKa = 4.11KK67 pKa = 10.91NDD69 pKa = 4.99KK70 pKa = 8.75ITNDD74 pKa = 3.11MDD76 pKa = 3.83TKK78 pKa = 10.82LFRR81 pKa = 11.84VKK83 pKa = 10.78SFMKK87 pKa = 10.3DD88 pKa = 2.72EE89 pKa = 5.02CYY91 pKa = 10.28PEE93 pKa = 4.21YY94 pKa = 10.96KK95 pKa = 9.73HH96 pKa = 6.74ARR98 pKa = 11.84AINSRR103 pKa = 11.84TDD105 pKa = 3.16EE106 pKa = 4.41FKK108 pKa = 10.66TLVGPTFKK116 pKa = 10.87LIEE119 pKa = 4.5KK120 pKa = 10.03EE121 pKa = 4.14LFEE124 pKa = 5.27LPWFIKK130 pKa = 10.1KK131 pKa = 10.04IPVKK135 pKa = 10.53DD136 pKa = 3.43RR137 pKa = 11.84PNYY140 pKa = 9.56IMEE143 pKa = 4.49RR144 pKa = 11.84LYY146 pKa = 10.94RR147 pKa = 11.84PNGKK151 pKa = 10.01YY152 pKa = 9.97YY153 pKa = 9.98ATDD156 pKa = 3.34YY157 pKa = 10.34TAFEE161 pKa = 3.97AHH163 pKa = 5.35FTKK166 pKa = 10.46PVMDD170 pKa = 3.81ACEE173 pKa = 3.9NRR175 pKa = 11.84LYY177 pKa = 10.91KK178 pKa = 10.96YY179 pKa = 7.57MTQHH183 pKa = 6.87LPNHH187 pKa = 6.48KK188 pKa = 10.3KK189 pKa = 10.42FMWYY193 pKa = 8.73IDD195 pKa = 3.57NVIGGRR201 pKa = 11.84NVCDD205 pKa = 4.99FKK207 pKa = 11.42HH208 pKa = 5.46LTVEE212 pKa = 3.95IDD214 pKa = 3.23ATRR217 pKa = 11.84MSGEE221 pKa = 4.25MNTSLGNGFSNLMLFLFMAHH241 pKa = 6.88EE242 pKa = 4.72KK243 pKa = 10.93GCTNVTGVVEE253 pKa = 4.37GDD255 pKa = 3.58DD256 pKa = 3.64GLFVLEE262 pKa = 4.74GPGITEE268 pKa = 4.06EE269 pKa = 4.14DD270 pKa = 3.32AKK272 pKa = 11.3EE273 pKa = 3.87CGFFLKK279 pKa = 10.22IEE281 pKa = 4.17SHH283 pKa = 6.58LSIATASFCGIIFDD297 pKa = 4.72EE298 pKa = 4.26EE299 pKa = 4.12DD300 pKa = 3.67RR301 pKa = 11.84NNLTNPLAEE310 pKa = 4.18LVNFGWTTGQYY321 pKa = 10.66ARR323 pKa = 11.84SKK325 pKa = 9.83RR326 pKa = 11.84ARR328 pKa = 11.84HH329 pKa = 5.75LEE331 pKa = 3.86LLRR334 pKa = 11.84SKK336 pKa = 10.73ALSLFYY342 pKa = 10.13QYY344 pKa = 10.26PGCPVLSSLANYY356 pKa = 10.23ALRR359 pKa = 11.84CTIGYY364 pKa = 8.15KK365 pKa = 10.52AKK367 pKa = 10.8LKK369 pKa = 10.18FFRR372 pKa = 11.84NMWEE376 pKa = 3.61RR377 pKa = 11.84EE378 pKa = 3.9QFIEE382 pKa = 3.97ALKK385 pKa = 10.8FKK387 pKa = 10.76DD388 pKa = 3.55EE389 pKa = 4.91AIVRR393 pKa = 11.84PVGNGSRR400 pKa = 11.84LLVEE404 pKa = 4.39EE405 pKa = 4.61LFGLSVTDD413 pKa = 3.45QLRR416 pKa = 11.84TEE418 pKa = 4.88AYY420 pKa = 10.3LDD422 pKa = 3.65SLDD425 pKa = 4.47TIQEE429 pKa = 4.05LDD431 pKa = 3.94MPWLEE436 pKa = 4.34WNYY439 pKa = 9.72HH440 pKa = 4.74KK441 pKa = 10.25HH442 pKa = 5.15WKK444 pKa = 9.96HH445 pKa = 5.62YY446 pKa = 9.89ADD448 pKa = 4.51NYY450 pKa = 10.13LAYY453 pKa = 9.74TDD455 pKa = 4.24GRR457 pKa = 11.84FKK459 pKa = 11.09ADD461 pKa = 3.13YY462 pKa = 10.13KK463 pKa = 10.56EE464 pKa = 3.89RR465 pKa = 11.84HH466 pKa = 5.8EE467 pKa = 4.19MSIHH471 pKa = 5.47SWTFTRR477 pKa = 11.84ACC479 pKa = 4.11

Molecular weight: 55.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGD7|A0A1L3KGD7_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 36 OX=1923284 PE=4 SV=1
MM1 pKa = 7.65NDD3 pKa = 2.65NHH5 pKa = 6.94SIKK8 pKa = 10.53RR9 pKa = 11.84PEE11 pKa = 3.92KK12 pKa = 9.21EE13 pKa = 3.74GARR16 pKa = 11.84GGNKK20 pKa = 8.72KK21 pKa = 10.3RR22 pKa = 11.84KK23 pKa = 9.2GDD25 pKa = 3.95PRR27 pKa = 11.84PKK29 pKa = 9.62IWVEE33 pKa = 3.83KK34 pKa = 9.85KK35 pKa = 10.86GNGAGGGQPKK45 pKa = 9.96RR46 pKa = 11.84RR47 pKa = 11.84EE48 pKa = 3.82EE49 pKa = 4.27SVRR52 pKa = 11.84DD53 pKa = 3.54AAWYY57 pKa = 7.54TEE59 pKa = 4.1KK60 pKa = 10.73NAKK63 pKa = 8.46LTFLLNEE70 pKa = 4.35GSISEE75 pKa = 3.95PRR77 pKa = 11.84KK78 pKa = 9.94RR79 pKa = 11.84EE80 pKa = 3.54LSAQYY85 pKa = 7.45EE86 pKa = 4.45TNLYY90 pKa = 9.24EE91 pKa = 4.32LHH93 pKa = 7.04KK94 pKa = 10.45LKK96 pKa = 10.44QRR98 pKa = 11.84NAMVQEE104 pKa = 3.71PRR106 pKa = 11.84MPDD109 pKa = 3.32KK110 pKa = 10.92QPEE113 pKa = 4.33LPPPAVARR121 pKa = 11.84SVEE124 pKa = 4.22PSAKK128 pKa = 8.89TAARR132 pKa = 11.84KK133 pKa = 10.1DD134 pKa = 3.17KK135 pKa = 11.07GGPRR139 pKa = 11.84KK140 pKa = 9.88QGTRR144 pKa = 11.84AATRR148 pKa = 11.84KK149 pKa = 9.78SAVEE153 pKa = 3.55KK154 pKa = 11.06SLINSEE160 pKa = 4.33AKK162 pKa = 10.65AKK164 pKa = 10.9GIDD167 pKa = 3.64DD168 pKa = 3.77ARR170 pKa = 11.84QDD172 pKa = 3.28RR173 pKa = 11.84VEE175 pKa = 4.29FARR178 pKa = 11.84QDD180 pKa = 3.58VQSANAILDD189 pKa = 3.65NLLQQQAAPKK199 pKa = 9.93PEE201 pKa = 4.4EE202 pKa = 4.12EE203 pKa = 5.45DD204 pKa = 3.27EE205 pKa = 4.31DD206 pKa = 4.0TSEE209 pKa = 4.39EE210 pKa = 4.2EE211 pKa = 4.08EE212 pKa = 4.45SVEE215 pKa = 3.97PKK217 pKa = 10.77APEE220 pKa = 3.78TTADD224 pKa = 3.07KK225 pKa = 10.32WLSDD229 pKa = 4.59LYY231 pKa = 10.93RR232 pKa = 11.84CDD234 pKa = 2.99WLGPHH239 pKa = 6.62SKK241 pKa = 9.01FTFWEE246 pKa = 4.49GRR248 pKa = 11.84AYY250 pKa = 10.58HH251 pKa = 6.41LTTIAARR258 pKa = 11.84LILATLLWLVLCSFICNSLQGIAYY282 pKa = 7.14TYY284 pKa = 10.98LSAHH288 pKa = 6.77DD289 pKa = 4.65ADD291 pKa = 4.05LTRR294 pKa = 11.84AKK296 pKa = 10.59ALEE299 pKa = 4.35CFVGKK304 pKa = 10.35YY305 pKa = 9.81DD306 pKa = 3.93QGRR309 pKa = 11.84SYY311 pKa = 8.27WTLGGGLTKK320 pKa = 10.52LDD322 pKa = 4.0VKK324 pKa = 10.89PGDD327 pKa = 3.47PYY329 pKa = 11.47YY330 pKa = 11.29DD331 pKa = 3.41HH332 pKa = 7.57FYY334 pKa = 10.87DD335 pKa = 4.61SSGSAIKK342 pKa = 10.12GAKK345 pKa = 9.64NIPTQRR351 pKa = 11.84IMEE354 pKa = 4.82SIRR357 pKa = 11.84KK358 pKa = 8.64LRR360 pKa = 11.84QSSANALTAYY370 pKa = 10.38NWISNLSTFLYY381 pKa = 8.63QGKK384 pKa = 9.25YY385 pKa = 7.24LTGEE389 pKa = 3.74AFFFIILFFLWGKK402 pKa = 9.79IYY404 pKa = 10.72HH405 pKa = 6.6SDD407 pKa = 3.62TVKK410 pKa = 10.64HH411 pKa = 6.25SYY413 pKa = 11.12HH414 pKa = 5.55NVEE417 pKa = 4.27YY418 pKa = 10.28FDD420 pKa = 4.03PKK422 pKa = 9.13TWRR425 pKa = 11.84ACDD428 pKa = 3.43KK429 pKa = 10.15DD430 pKa = 3.87APGAVTADD438 pKa = 3.69YY439 pKa = 10.84FNTARR444 pKa = 11.84DD445 pKa = 3.47MRR447 pKa = 11.84NDD449 pKa = 2.76VSARR453 pKa = 11.84SKK455 pKa = 10.92KK456 pKa = 7.93EE457 pKa = 3.6HH458 pKa = 6.28EE459 pKa = 4.34PLYY462 pKa = 10.2FAYY465 pKa = 9.35TRR467 pKa = 11.84HH468 pKa = 5.99EE469 pKa = 4.15EE470 pKa = 3.9HH471 pKa = 5.98WWYY474 pKa = 10.37IGVGRR479 pKa = 11.84MKK481 pKa = 10.68VPVFRR486 pKa = 11.84HH487 pKa = 4.92IKK489 pKa = 9.75KK490 pKa = 10.07VPNVKK495 pKa = 10.03LSSMEE500 pKa = 4.21LVFQICHH507 pKa = 6.44PFNMEE512 pKa = 3.02WTAPEE517 pKa = 5.07DD518 pKa = 3.35ICIDD522 pKa = 3.76RR523 pKa = 11.84MTKK526 pKa = 8.92TKK528 pKa = 9.68NTTQSININKK538 pKa = 9.0QLIITKK544 pKa = 9.88QDD546 pKa = 2.86VFEE549 pKa = 4.19NSRR552 pKa = 11.84AISLGFFYY560 pKa = 10.9GLRR563 pKa = 11.84QDD565 pKa = 3.56HH566 pKa = 6.66SKK568 pKa = 10.92SAFQLAPEE576 pKa = 4.95LKK578 pKa = 10.22AAYY581 pKa = 10.04QFDD584 pKa = 4.19FLHH587 pKa = 6.76TDD589 pKa = 3.36TVDD592 pKa = 3.45TRR594 pKa = 11.84CLYY597 pKa = 11.35LMTQISGLARR607 pKa = 11.84AYY609 pKa = 10.21RR610 pKa = 11.84SYY612 pKa = 11.61VHH614 pKa = 6.6MGQTLVSLCLFLWAYY629 pKa = 7.43MM630 pKa = 3.96

Molecular weight: 72.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1109

479

630

554.5

64.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.665 ± 0.888

1.623 ± 0.141

5.861 ± 0.107

6.943 ± 0.316

4.959 ± 0.604

5.5 ± 0.04

2.795 ± 0.07

4.418 ± 0.134

7.935 ± 0.116

9.017 ± 0.554

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.319

4.418 ± 0.327

4.418 ± 0.134

3.156 ± 0.704

6.222 ± 0.092

5.591 ± 0.665

6.222 ± 0.253

4.148 ± 0.015

2.164 ± 0.042

4.599 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski