Streptomyces phage Diane

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291LHK8|A0A291LHK8_9CAUD Uncharacterized protein OS=Streptomyces phage Diane OX=2041207 GN=SEA_DIANE_75 PE=4 SV=1
MM1 pKa = 7.33EE2 pKa = 6.39LDD4 pKa = 3.81RR5 pKa = 11.84EE6 pKa = 4.43TLEE9 pKa = 4.32KK10 pKa = 10.51AAEE13 pKa = 4.11EE14 pKa = 4.11ALSRR18 pKa = 11.84PSDD21 pKa = 3.14AMFWDD26 pKa = 3.91DD27 pKa = 5.82RR28 pKa = 11.84LFTTHH33 pKa = 6.65GAVFHH38 pKa = 6.07WAEE41 pKa = 4.51LGDD44 pKa = 4.54DD45 pKa = 4.3LLEE48 pKa = 4.27EE49 pKa = 4.61SNYY52 pKa = 8.88LTALDD57 pKa = 5.31LIRR60 pKa = 11.84GAAGDD65 pKa = 3.77FADD68 pKa = 3.72EE69 pKa = 4.25HH70 pKa = 8.17VIDD73 pKa = 4.32GASRR77 pKa = 11.84HH78 pKa = 4.89WACGSLRR85 pKa = 11.84TIYY88 pKa = 9.71VQVYY92 pKa = 6.57EE93 pKa = 4.31TYY95 pKa = 10.6EE96 pKa = 4.26DD97 pKa = 4.3EE98 pKa = 4.44EE99 pKa = 5.25CEE101 pKa = 4.09CEE103 pKa = 3.81PAFEE107 pKa = 5.82HH108 pKa = 7.65EE109 pKa = 5.0DD110 pKa = 3.62GCDD113 pKa = 3.31QDD115 pKa = 3.71EE116 pKa = 4.98DD117 pKa = 3.4SWYY120 pKa = 9.39CQNYY124 pKa = 9.52CLYY127 pKa = 10.07EE128 pKa = 5.25CDD130 pKa = 5.38GEE132 pKa = 4.39QCLPEE137 pKa = 4.3EE138 pKa = 4.85LEE140 pKa = 3.84FTAAFKK146 pKa = 10.54EE147 pKa = 4.23ATEE150 pKa = 3.98LAVYY154 pKa = 10.32LRR156 pKa = 11.84DD157 pKa = 3.6GGAVLDD163 pKa = 4.66DD164 pKa = 3.55SDD166 pKa = 4.11YY167 pKa = 11.75SEE169 pKa = 5.39RR170 pKa = 11.84EE171 pKa = 3.49WEE173 pKa = 4.17AFEE176 pKa = 4.71KK177 pKa = 10.61ALKK180 pKa = 10.04EE181 pKa = 4.17AVEE184 pKa = 4.13HH185 pKa = 5.9AQRR188 pKa = 11.84EE189 pKa = 4.35YY190 pKa = 11.31TLVDD194 pKa = 3.35TCAEE198 pKa = 4.15DD199 pKa = 3.99DD200 pKa = 5.16AIAEE204 pKa = 4.81LYY206 pKa = 9.63YY207 pKa = 10.17QDD209 pKa = 4.03EE210 pKa = 4.92GNAHH214 pKa = 5.62RR215 pKa = 11.84QNWCRR220 pKa = 11.84AEE222 pKa = 4.34DD223 pKa = 3.79VDD225 pKa = 3.59WDD227 pKa = 4.23VVAEE231 pKa = 4.53EE232 pKa = 3.98YY233 pKa = 10.8RR234 pKa = 11.84EE235 pKa = 4.15ARR237 pKa = 11.84DD238 pKa = 3.34AYY240 pKa = 9.43FLEE243 pKa = 4.91RR244 pKa = 11.84ATEE247 pKa = 4.16VYY249 pKa = 9.45RR250 pKa = 11.84WNVLGYY256 pKa = 10.83NPDD259 pKa = 3.49QLEE262 pKa = 4.11LFAAA266 pKa = 4.76

Molecular weight: 30.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291LIC8|A0A291LIC8_9CAUD Major capsid protein OS=Streptomyces phage Diane OX=2041207 GN=SEA_DIANE_10 PE=4 SV=1
MM1 pKa = 7.01TPKK4 pKa = 10.14FRR6 pKa = 11.84IHH8 pKa = 6.69DD9 pKa = 3.9TNVRR13 pKa = 11.84DD14 pKa = 4.61SKK16 pKa = 11.19RR17 pKa = 11.84KK18 pKa = 10.09DD19 pKa = 3.19KK20 pKa = 11.61AEE22 pKa = 3.77TLARR26 pKa = 11.84RR27 pKa = 11.84EE28 pKa = 4.01VRR30 pKa = 11.84RR31 pKa = 11.84TKK33 pKa = 10.87YY34 pKa = 9.66EE35 pKa = 4.17SNPAVVRR42 pKa = 11.84IAAAA46 pKa = 3.48

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15544

35

1302

196.8

21.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.988 ± 0.474

0.836 ± 0.14

6.53 ± 0.293

7.373 ± 0.461

2.959 ± 0.194

8.254 ± 0.328

2.014 ± 0.185

4.355 ± 0.268

4.722 ± 0.337

8.608 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.138

2.689 ± 0.137

4.761 ± 0.235

3.487 ± 0.186

6.44 ± 0.447

6.157 ± 0.369

6.15 ± 0.29

6.935 ± 0.222

1.782 ± 0.109

2.889 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski