Synechococcus phage S-B64

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GL23|A0A2S1GL23_9CAUD Uncharacterized protein OS=Synechococcus phage S-B64 OX=2163901 PE=4 SV=1
MM1 pKa = 7.75YY2 pKa = 10.51ILTDD6 pKa = 3.46KK7 pKa = 10.3NTGGVYY13 pKa = 10.42AVFDD17 pKa = 3.86KK18 pKa = 11.33DD19 pKa = 3.31RR20 pKa = 11.84VKK22 pKa = 9.81TVQVFEE28 pKa = 4.86EE29 pKa = 4.12EE30 pKa = 4.13DD31 pKa = 3.29DD32 pKa = 4.46AIRR35 pKa = 11.84YY36 pKa = 8.81QEE38 pKa = 5.19LLLADD43 pKa = 5.25DD44 pKa = 5.56LDD46 pKa = 5.91DD47 pKa = 4.9DD48 pKa = 4.81LEE50 pKa = 4.44VMEE53 pKa = 5.02VEE55 pKa = 4.55LSTVAVNCEE64 pKa = 3.84NYY66 pKa = 10.19GYY68 pKa = 9.45GYY70 pKa = 10.75SVITSNDD77 pKa = 2.87FVIPPNQQ84 pKa = 3.08

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GL97|A0A2S1GL97_9CAUD DNA primase subunit OS=Synechococcus phage S-B64 OX=2163901 PE=4 SV=1
MM1 pKa = 6.99TTPNWQHH8 pKa = 5.99HH9 pKa = 5.53SKK11 pKa = 10.91KK12 pKa = 9.31EE13 pKa = 3.94AKK15 pKa = 8.62RR16 pKa = 11.84TLKK19 pKa = 10.42PQALRR24 pKa = 11.84QAKK27 pKa = 9.23KK28 pKa = 10.21RR29 pKa = 11.84RR30 pKa = 11.84AALKK34 pKa = 10.48AKK36 pKa = 10.33LLASTVALVGFASPAQAVTWGEE58 pKa = 3.43FWEE61 pKa = 4.54PFKK64 pKa = 11.07SDD66 pKa = 3.21HH67 pKa = 6.21HH68 pKa = 6.15HH69 pKa = 5.75HH70 pKa = 7.13HH71 pKa = 5.6YY72 pKa = 10.0HH73 pKa = 5.43HH74 pKa = 7.08RR75 pKa = 11.84PRR77 pKa = 11.84RR78 pKa = 11.84AMCEE82 pKa = 3.44HH83 pKa = 6.61RR84 pKa = 11.84EE85 pKa = 3.92YY86 pKa = 11.28RR87 pKa = 11.84EE88 pKa = 3.86VRR90 pKa = 11.84DD91 pKa = 3.44SRR93 pKa = 11.84GRR95 pKa = 11.84FVDD98 pKa = 3.75YY99 pKa = 10.44YY100 pKa = 9.97YY101 pKa = 10.18EE102 pKa = 4.31TVRR105 pKa = 11.84EE106 pKa = 4.16PCWRR110 pKa = 11.84KK111 pKa = 9.2YY112 pKa = 10.19HH113 pKa = 6.75DD114 pKa = 3.64HH115 pKa = 6.8

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

156

0

156

38363

36

2407

245.9

27.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.184 ± 0.321

0.933 ± 0.091

6.678 ± 0.191

6.592 ± 0.363

4.418 ± 0.156

7.333 ± 0.348

1.569 ± 0.112

6.084 ± 0.159

6.339 ± 0.455

7.33 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.177

5.706 ± 0.218

3.962 ± 0.204

3.699 ± 0.089

4.293 ± 0.134

6.47 ± 0.251

6.973 ± 0.467

6.728 ± 0.183

1.204 ± 0.099

4.249 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski