Bacteriophage APSE-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sendosyvirus; Hamiltonella virus APSE2

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6SCW0|B6SCW0_9CAUD DNA stabilization gp10 OS=Bacteriophage APSE-2 OX=340054 GN=P28 PE=4 SV=1
MM1 pKa = 7.57TKK3 pKa = 10.19PLTKK7 pKa = 10.75GEE9 pKa = 4.11IVLFALRR16 pKa = 11.84KK17 pKa = 9.96AGIASEE23 pKa = 4.06ATNIDD28 pKa = 3.54VEE30 pKa = 4.33PQSFEE35 pKa = 3.96EE36 pKa = 5.28GINDD40 pKa = 5.03LEE42 pKa = 4.46DD43 pKa = 4.59LMAEE47 pKa = 4.07LQITFGDD54 pKa = 3.66LGYY57 pKa = 10.61QFSAEE62 pKa = 4.32EE63 pKa = 4.11EE64 pKa = 4.27NPTADD69 pKa = 4.45DD70 pKa = 4.02ASGLPRR76 pKa = 11.84KK77 pKa = 9.08YY78 pKa = 9.79KK79 pKa = 10.43QVMGYY84 pKa = 9.4QLMLRR89 pKa = 11.84MLSDD93 pKa = 3.68YY94 pKa = 10.87GIEE97 pKa = 3.79PTPRR101 pKa = 11.84QEE103 pKa = 3.96ASAAAAYY110 pKa = 8.09DD111 pKa = 3.69ALLTDD116 pKa = 4.15TLSVPSIARR125 pKa = 11.84RR126 pKa = 11.84GDD128 pKa = 3.4MPVGQGNNYY137 pKa = 7.5TALGTASYY145 pKa = 9.24YY146 pKa = 9.37VEE148 pKa = 4.41RR149 pKa = 11.84GFHH152 pKa = 6.71AKK154 pKa = 10.13NADD157 pKa = 3.66PVSS160 pKa = 3.44

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6SCU4|B6SCU4_9CAUD Q protein OS=Bacteriophage APSE-2 OX=340054 GN=P5 PE=3 SV=1
MM1 pKa = 6.82HH2 pKa = 7.06TKK4 pKa = 10.16HH5 pKa = 6.54YY6 pKa = 10.38IEE8 pKa = 5.15GYY10 pKa = 9.68RR11 pKa = 11.84YY12 pKa = 10.18LIYY15 pKa = 10.15IYY17 pKa = 10.58LIGKK21 pKa = 9.39RR22 pKa = 11.84LLRR25 pKa = 11.84LLRR28 pKa = 11.84LLHH31 pKa = 5.92SFKK34 pKa = 10.65INKK37 pKa = 9.36LKK39 pKa = 10.82RR40 pKa = 11.84NNLLLIGCCRR50 pKa = 11.84LLRR53 pKa = 11.84LRR55 pKa = 11.84TVKK58 pKa = 10.21IFPFITGAKK67 pKa = 9.19KK68 pKa = 10.03HH69 pKa = 5.37EE70 pKa = 4.14QRR72 pKa = 11.84HH73 pKa = 4.35

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11450

73

993

279.3

31.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.751 ± 0.49

1.039 ± 0.172

5.563 ± 0.257

6.096 ± 0.325

3.502 ± 0.235

6.533 ± 0.366

2.122 ± 0.158

6.026 ± 0.227

6.934 ± 0.301

8.786 ± 0.347

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.154

5.153 ± 0.293

4.087 ± 0.206

4.838 ± 0.337

5.424 ± 0.285

6.541 ± 0.278

5.59 ± 0.278

5.493 ± 0.218

1.546 ± 0.218

3.336 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski