Candidatus Regiella insecticola LSR1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; aphid secondary symbionts; Candidatus Regiella; Candidatus Regiella insecticola

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1368 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0WV44|E0WV44_9ENTR Putative plasmid assoicated protein OS=Candidatus Regiella insecticola LSR1 OX=663321 GN=REG_1959 PE=4 SV=1
MM1 pKa = 7.62FYY3 pKa = 10.64EE4 pKa = 4.02QDD6 pKa = 3.43FDD8 pKa = 4.24QLQAGNDD15 pKa = 3.19NFTWHH20 pKa = 6.07VALSDD25 pKa = 4.59PLPEE29 pKa = 5.38DD30 pKa = 2.86NWSGYY35 pKa = 8.52TGFIHH40 pKa = 6.84NVLLEE45 pKa = 4.48NYY47 pKa = 9.74LKK49 pKa = 10.7DD50 pKa = 3.42HH51 pKa = 7.35PAPEE55 pKa = 4.37DD56 pKa = 3.67CEE58 pKa = 5.73FYY60 pKa = 10.28MCGPPMMNAAVISMLKK76 pKa = 10.09NLGVEE81 pKa = 4.84DD82 pKa = 4.41EE83 pKa = 5.42NIMLDD88 pKa = 4.04DD89 pKa = 4.31FGGG92 pKa = 3.45

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0WTP1|E0WTP1_9ENTR Uncharacterized protein OS=Candidatus Regiella insecticola LSR1 OX=663321 GN=REG_1411 PE=4 SV=1
MM1 pKa = 7.88LPQCNMKK8 pKa = 10.14SRR10 pKa = 11.84CRR12 pKa = 11.84YY13 pKa = 8.73GFTHH17 pKa = 7.19RR18 pKa = 11.84RR19 pKa = 11.84LQHH22 pKa = 6.15LPCGGKK28 pKa = 7.6TLILCFRR35 pKa = 11.84LQRR38 pKa = 11.84FCCTNSRR45 pKa = 11.84CPQRR49 pKa = 11.84TFQEE53 pKa = 4.68SISTLAPRR61 pKa = 11.84WQRR64 pKa = 11.84SSRR67 pKa = 11.84QLVEE71 pKa = 3.6MTTRR75 pKa = 11.84LAYY78 pKa = 10.42SFGVRR83 pKa = 11.84VSGDD87 pKa = 3.24TLIRR91 pKa = 11.84RR92 pKa = 11.84IMRR95 pKa = 11.84LPLSEE100 pKa = 4.78PEE102 pKa = 4.07QLTHH106 pKa = 7.61IGVDD110 pKa = 3.06DD111 pKa = 3.85WAWRR115 pKa = 11.84KK116 pKa = 8.0GHH118 pKa = 6.8HH119 pKa = 6.54YY120 pKa = 9.44GTIIVDD126 pKa = 3.69QSTHH130 pKa = 6.39RR131 pKa = 11.84PVALLSGRR139 pKa = 11.84SATSLAEE146 pKa = 3.85WLKK149 pKa = 9.48TRR151 pKa = 11.84PEE153 pKa = 3.71IKK155 pKa = 9.96IITRR159 pKa = 11.84DD160 pKa = 3.25RR161 pKa = 11.84AGAYY165 pKa = 10.33ADD167 pKa = 3.64VAKK170 pKa = 9.96TGASQAIQIADD181 pKa = 3.6RR182 pKa = 11.84WHH184 pKa = 7.24LIRR187 pKa = 11.84NAGDD191 pKa = 3.02HH192 pKa = 6.38FEE194 pKa = 4.73RR195 pKa = 11.84FLKK198 pKa = 10.18RR199 pKa = 11.84HH200 pKa = 4.26WVAVKK205 pKa = 8.59EE206 pKa = 4.41TYY208 pKa = 9.89HH209 pKa = 7.08AIKK212 pKa = 8.68TQEE215 pKa = 3.78LSALQSEE222 pKa = 5.06PIITVSPQQEE232 pKa = 4.25TVTQQQRR239 pKa = 11.84RR240 pKa = 11.84LLFEE244 pKa = 5.2HH245 pKa = 7.2ISVLKK250 pKa = 9.93NKK252 pKa = 9.55GMAQTEE258 pKa = 4.08IALTAGISDD267 pKa = 3.43KK268 pKa = 9.25TVRR271 pKa = 11.84RR272 pKa = 11.84WLQTGGPPLCGKK284 pKa = 10.1RR285 pKa = 11.84PRR287 pKa = 11.84PRR289 pKa = 11.84QVIGFNEE296 pKa = 4.24QTWLRR301 pKa = 11.84EE302 pKa = 3.97QGNSGCHH309 pKa = 4.48NAALLFRR316 pKa = 11.84KK317 pKa = 9.82LKK319 pKa = 8.31TRR321 pKa = 11.84GYY323 pKa = 9.04VGGSATVRR331 pKa = 11.84SLVSQWRR338 pKa = 11.84YY339 pKa = 7.37TSAEE343 pKa = 4.07VLQYY347 pKa = 11.14PPMPRR352 pKa = 11.84LKK354 pKa = 10.36QVMQWLTSPLNLDD367 pKa = 3.36KK368 pKa = 11.34SGAATWIKK376 pKa = 10.71RR377 pKa = 11.84FVQQLLTLCPGLNVGCKK394 pKa = 8.46LTQGFISLFSPSTKK408 pKa = 9.13IKK410 pKa = 10.03EE411 pKa = 3.99RR412 pKa = 11.84SRR414 pKa = 11.84LLRR417 pKa = 11.84QWLHH421 pKa = 6.04EE422 pKa = 4.05ATHH425 pKa = 6.59CKK427 pKa = 10.12LSEE430 pKa = 4.06YY431 pKa = 10.86QKK433 pKa = 9.71MASSLEE439 pKa = 4.03MEE441 pKa = 4.88LDD443 pKa = 3.44AVEE446 pKa = 4.25NALTHH451 pKa = 6.01RR452 pKa = 11.84WSNGVVEE459 pKa = 4.11GHH461 pKa = 5.2VNRR464 pKa = 11.84LKK466 pKa = 9.78MLKK469 pKa = 9.26RR470 pKa = 11.84QMYY473 pKa = 8.84GRR475 pKa = 11.84AGFEE479 pKa = 3.89LLSRR483 pKa = 11.84RR484 pKa = 11.84VMNTLCC490 pKa = 4.7

Molecular weight: 56.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1368

0

1368

389023

30

1486

284.4

31.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.256 ± 0.058

1.211 ± 0.024

5.062 ± 0.055

5.758 ± 0.059

3.839 ± 0.044

6.241 ± 0.062

2.417 ± 0.033

7.11 ± 0.067

6.045 ± 0.059

10.789 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.032

4.592 ± 0.046

4.058 ± 0.046

4.786 ± 0.056

5.338 ± 0.053

6.16 ± 0.051

5.347 ± 0.042

6.155 ± 0.055

1.21 ± 0.029

2.95 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski