Cryptosporidium parvum (strain Iowa II)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Cryptosporidiidae; Cryptosporidium; Cryptosporidium parvum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3FPS3|A3FPS3_CRYPI Casein kinase II alpha subunit putative OS=Cryptosporidium parvum (strain Iowa II) OX=353152 GN=cgd6_620 PE=3 SV=1
MM1 pKa = 8.11DD2 pKa = 5.75PLEE5 pKa = 4.86SDD7 pKa = 3.76GTVLLPQIFVDD18 pKa = 5.66GVCIGNDD25 pKa = 3.0VSLQDD30 pKa = 4.8LEE32 pKa = 4.52EE33 pKa = 5.95DD34 pKa = 4.84NDD36 pKa = 3.87FDD38 pKa = 4.64WIINRR43 pKa = 11.84RR44 pKa = 11.84ACGACLSDD52 pKa = 4.1KK53 pKa = 11.02EE54 pKa = 4.4EE55 pKa = 4.49DD56 pKa = 3.71MEE58 pKa = 4.83VCPNCNQVFKK68 pKa = 10.48TIVPPEE74 pKa = 3.95LVYY77 pKa = 11.1SGGIQQMYY85 pKa = 9.7RR86 pKa = 11.84GNNTAAQEE94 pKa = 4.4GVLFEE99 pKa = 4.88HH100 pKa = 7.0PTNFVKK106 pKa = 10.93NNNSNLNLNRR116 pKa = 11.84NEE118 pKa = 4.42RR119 pKa = 11.84YY120 pKa = 10.12GDD122 pKa = 3.66YY123 pKa = 10.33QDD125 pKa = 4.22EE126 pKa = 4.35EE127 pKa = 4.9EE128 pKa = 4.4YY129 pKa = 11.53DD130 pKa = 3.77NIAEE134 pKa = 4.17EE135 pKa = 4.99EE136 pKa = 4.18YY137 pKa = 11.06SEE139 pKa = 6.24LDD141 pKa = 3.48IKK143 pKa = 11.34DD144 pKa = 3.7DD145 pKa = 3.64

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5CPE3|Q5CPE3_CRYPI Uncharacterized protein OS=Cryptosporidium parvum (strain Iowa II) OX=353152 GN=cgd5_4610 PE=4 SV=1
MM1 pKa = 7.34NLALNNVICNSRR13 pKa = 11.84DD14 pKa = 3.24GNGFFKK20 pKa = 10.39MVEE23 pKa = 4.02CYY25 pKa = 10.11IRR27 pKa = 11.84GNNIKK32 pKa = 10.38LIRR35 pKa = 11.84ISDD38 pKa = 3.81EE39 pKa = 4.47NINTAKK45 pKa = 10.86DD46 pKa = 3.34DD47 pKa = 3.5MTQRR51 pKa = 11.84EE52 pKa = 4.31TARR55 pKa = 11.84LGNRR59 pKa = 11.84GRR61 pKa = 11.84PRR63 pKa = 11.84NEE65 pKa = 3.06TRR67 pKa = 11.84ASRR70 pKa = 11.84VRR72 pKa = 11.84SSKK75 pKa = 9.43WVV77 pKa = 3.11

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3805

0

3805

2271432

49

13413

597.0

68.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.415 ± 0.044

1.711 ± 0.019

5.176 ± 0.024

7.053 ± 0.037

4.888 ± 0.034

4.695 ± 0.047

1.842 ± 0.013

8.872 ± 0.047

7.769 ± 0.04

9.608 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.909 ± 0.012

8.125 ± 0.059

3.453 ± 0.036

3.623 ± 0.029

3.981 ± 0.033

9.964 ± 0.042

4.746 ± 0.045

4.617 ± 0.029

0.882 ± 0.009

3.656 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski