Mangrovibacter sp. MFB070

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Mangrovibacter; unclassified Mangrovibacter

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A071LY43|A0A071LY43_9ENTR Transcriptional regulator OS=Mangrovibacter sp. MFB070 OX=1224318 GN=DT73_06345 PE=4 SV=1
MM1 pKa = 7.54FKK3 pKa = 9.24QTVIAAAIIFSGAAFATEE21 pKa = 4.23STGVAGGTITFNGSVSDD38 pKa = 3.86TTCDD42 pKa = 3.04VTTNNGSDD50 pKa = 3.45FTVNLTPVTLDD61 pKa = 4.99DD62 pKa = 4.06INSAAGVVAAGKK74 pKa = 10.38KK75 pKa = 10.31DD76 pKa = 3.38FTMSLKK82 pKa = 10.56NCSAADD88 pKa = 4.03DD89 pKa = 4.35GATTLKK95 pKa = 9.25ITFSSSNLSDD105 pKa = 3.55DD106 pKa = 3.72GKK108 pKa = 10.92YY109 pKa = 10.54LKK111 pKa = 10.64NYY113 pKa = 9.98SDD115 pKa = 3.95GGADD119 pKa = 3.34GVGIALTSDD128 pKa = 3.32GSTLVPFDD136 pKa = 3.76SAFDD140 pKa = 3.74TGLSSTDD147 pKa = 3.28VASTNGATVTMYY159 pKa = 11.34ANYY162 pKa = 10.71YY163 pKa = 10.16NFGNGAATTGKK174 pKa = 10.03VVTDD178 pKa = 3.32ATYY181 pKa = 11.16AFSYY185 pKa = 10.7DD186 pKa = 3.18

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A071MCS9|A0A071MCS9_9ENTR Uncharacterized protein OS=Mangrovibacter sp. MFB070 OX=1224318 GN=DT73_02760 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84AVTVFWHH9 pKa = 6.51RR10 pKa = 11.84AVCVCVHH17 pKa = 5.93TARR20 pKa = 11.84VCFFFEE26 pKa = 4.67SFFSEE31 pKa = 4.03LMLYY35 pKa = 9.82PGAVTFRR42 pKa = 11.84CSVNILLLVNSLFHH56 pKa = 6.4PRR58 pKa = 11.84PFLRR62 pKa = 11.84LSASHH67 pKa = 6.26YY68 pKa = 10.99SVFVCIKK75 pKa = 9.7RR76 pKa = 11.84KK77 pKa = 9.86LLIAIIKK84 pKa = 9.7RR85 pKa = 3.56

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4781

0

4781

1517325

30

3577

317.4

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.865 ± 0.039

1.141 ± 0.012

5.079 ± 0.029

5.381 ± 0.035

3.864 ± 0.023

7.53 ± 0.035

2.407 ± 0.02

5.618 ± 0.03

3.941 ± 0.028

10.743 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.017

3.766 ± 0.024

4.568 ± 0.024

4.719 ± 0.029

5.471 ± 0.031

6.135 ± 0.03

5.491 ± 0.035

7.281 ± 0.033

1.571 ± 0.017

2.739 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski