Achromobacter phage 83-24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Steinhofvirus; Achromobacter virus 83-24

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5A1X8|A0A0B5A1X8_9CAUD Uncharacterized protein OS=Achromobacter phage 83-24 OX=1589747 GN=JWAP_00051 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 4.51YY3 pKa = 9.21KK4 pKa = 10.78TLLEE8 pKa = 4.53KK9 pKa = 10.85YY10 pKa = 9.21VSHH13 pKa = 6.33VLQMEE18 pKa = 4.49GTTFIGWLNSGRR30 pKa = 11.84LSEE33 pKa = 4.43VEE35 pKa = 4.25FTAEE39 pKa = 3.56EE40 pKa = 4.21AEE42 pKa = 4.06EE43 pKa = 3.84LRR45 pKa = 11.84RR46 pKa = 11.84IGTEE50 pKa = 3.65FGDD53 pKa = 3.98DD54 pKa = 3.27DD55 pKa = 5.34AYY57 pKa = 11.1

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5A6W8|A0A0B5A6W8_9CAUD Uncharacterized protein OS=Achromobacter phage 83-24 OX=1589747 GN=JWAP_00054 PE=4 SV=1
MM1 pKa = 6.99TLSRR5 pKa = 11.84KK6 pKa = 8.94DD7 pKa = 3.37EE8 pKa = 3.95RR9 pKa = 11.84RR10 pKa = 11.84VRR12 pKa = 11.84EE13 pKa = 3.93LKK15 pKa = 10.3QVLEE19 pKa = 4.41YY20 pKa = 10.98AQTVPNRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84SFYY32 pKa = 10.76QHH34 pKa = 7.01TIQAAVRR41 pKa = 11.84EE42 pKa = 4.41MIYY45 pKa = 10.79NRR47 pKa = 11.84LVAFSQEE54 pKa = 3.94QLRR57 pKa = 11.84KK58 pKa = 9.85KK59 pKa = 10.09LASEE63 pKa = 4.22KK64 pKa = 9.36GTGRR68 pKa = 11.84ATAA71 pKa = 4.06

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

14004

46

1163

245.7

27.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.497 ± 0.409

0.85 ± 0.139

5.62 ± 0.323

6.455 ± 0.371

3.777 ± 0.185

7.926 ± 0.425

1.799 ± 0.199

4.984 ± 0.22

5.284 ± 0.475

8.433 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.685 ± 0.162

4.099 ± 0.249

5.077 ± 0.302

4.063 ± 0.276

5.948 ± 0.218

5.92 ± 0.266

5.684 ± 0.317

6.977 ± 0.245

1.835 ± 0.217

3.085 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski