Lachnospira multipara

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnospira

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5RPT9|A0A1H5RPT9_9FIRM Ribosomal protein S12 methylthiotransferase RimO OS=Lachnospira multipara OX=28051 GN=rimO PE=3 SV=1
MM1 pKa = 7.41SGIYY5 pKa = 9.96GVEE8 pKa = 3.76ALDD11 pKa = 3.71GNIYY15 pKa = 7.8QVKK18 pKa = 9.92YY19 pKa = 10.79SNDD22 pKa = 3.64SNSVTGDD29 pKa = 3.05SFDD32 pKa = 3.86TVFSEE37 pKa = 4.09INAPVDD43 pKa = 3.55LEE45 pKa = 4.95SIFSEE50 pKa = 4.13AASKK54 pKa = 11.3YY55 pKa = 9.81GVNEE59 pKa = 3.95NLLKK63 pKa = 10.7AVAYY67 pKa = 10.2AEE69 pKa = 4.72SGFDD73 pKa = 3.53TNATSSAGAQGIMQLMPYY91 pKa = 9.34VAEE94 pKa = 4.65DD95 pKa = 3.67LGVTDD100 pKa = 5.56PYY102 pKa = 11.25NAEE105 pKa = 3.91QNINAGASLLSSLLSKK121 pKa = 11.36YY122 pKa = 10.18NGNVTLSLAAYY133 pKa = 8.93NAGSGNVSKK142 pKa = 11.43YY143 pKa = 10.83NGVPPFKK150 pKa = 9.91EE151 pKa = 4.12TQNYY155 pKa = 7.83IAKK158 pKa = 10.31INDD161 pKa = 4.01LLGGALSGDD170 pKa = 3.71STTLDD175 pKa = 4.38GISPATVIEE184 pKa = 4.21EE185 pKa = 4.28SLVDD189 pKa = 3.59IPSTKK194 pKa = 9.61IYY196 pKa = 10.79SNSNSQTNEE205 pKa = 3.11EE206 pKa = 4.18STQLNTNANAISNSAYY222 pKa = 9.52LTVANNEE229 pKa = 4.16KK230 pKa = 10.63LLEE233 pKa = 4.08LLKK236 pKa = 11.09ALANSGSNSSTYY248 pKa = 10.12KK249 pKa = 10.69DD250 pKa = 3.55SVMLDD255 pKa = 3.72LVSLITGNYY264 pKa = 10.28DD265 pKa = 3.07SDD267 pKa = 4.14EE268 pKa = 4.35DD269 pKa = 4.16SKK271 pKa = 12.01DD272 pKa = 4.03LISSLYY278 pKa = 8.97TNSATSTVSDD288 pKa = 3.68SDD290 pKa = 3.6EE291 pKa = 4.54SLLSSYY297 pKa = 10.76LSSLSTDD304 pKa = 3.46EE305 pKa = 5.97LDD307 pKa = 4.19ALSKK311 pKa = 10.87NSQLQEE317 pKa = 4.03DD318 pKa = 4.72FGLSSEE324 pKa = 4.32ALALTSKK331 pKa = 10.92LNNQANNILEE341 pKa = 4.22TDD343 pKa = 4.18LYY345 pKa = 11.24SLYY348 pKa = 10.06EE349 pKa = 4.18AQSSVMSPVLAKK361 pKa = 10.68LLNLL365 pKa = 4.1

Molecular weight: 38.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5U848|A0A1H5U848_9FIRM Energy-coupling factor transport system ATP-binding protein OS=Lachnospira multipara OX=28051 GN=SAMN05216537_106107 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.43TRR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.26VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.61NLSAA44 pKa = 4.67

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2518

0

2518

824386

32

2035

327.4

36.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.897 ± 0.05

1.286 ± 0.018

6.303 ± 0.038

7.474 ± 0.056

4.238 ± 0.038

6.278 ± 0.049

1.413 ± 0.018

8.284 ± 0.055

7.854 ± 0.047

8.777 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.025

5.751 ± 0.039

2.653 ± 0.028

2.352 ± 0.027

3.592 ± 0.033

6.568 ± 0.054

5.565 ± 0.052

6.849 ± 0.044

0.705 ± 0.015

4.496 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski