Chloropicon primus

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Chloropicophyceae; Chloropicales; Chloropicaceae;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8MPD5|A0A5B8MPD5_9CHLO Transcription initiation factor IIF subunit beta OS=Chloropicon primus OX=1764295 GN=A3770_07p48870 PE=3 SV=1
MM1 pKa = 6.98VHH3 pKa = 5.69TSKK6 pKa = 11.25YY7 pKa = 9.76LAASLALALAVAGGAVAQDD26 pKa = 3.41QQDD29 pKa = 3.41FGDD32 pKa = 3.72AMRR35 pKa = 11.84QFLAHH40 pKa = 6.53SANGTCEE47 pKa = 5.51DD48 pKa = 4.38IPQEE52 pKa = 3.92MWNTTMRR59 pKa = 11.84NGTNQDD65 pKa = 3.59CEE67 pKa = 4.53GWWQSIAGDD76 pKa = 3.86ARR78 pKa = 11.84SVADD82 pKa = 5.22DD83 pKa = 3.26IGQAFEE89 pKa = 4.34NGAPLLVNGTEE100 pKa = 4.37KK101 pKa = 10.34ICVNSMPLDD110 pKa = 4.46DD111 pKa = 4.63YY112 pKa = 11.8SQIVQSACWPSGMEE126 pKa = 4.01EE127 pKa = 4.03EE128 pKa = 4.78PEE130 pKa = 3.89EE131 pKa = 3.96NHH133 pKa = 6.4AVRR136 pKa = 11.84IRR138 pKa = 11.84LGLPLQCVDD147 pKa = 3.75AADD150 pKa = 4.48YY151 pKa = 11.32DD152 pKa = 5.25DD153 pKa = 4.05STDD156 pKa = 3.15WSDD159 pKa = 4.15VPLQAYY165 pKa = 7.34MVSGEE170 pKa = 4.15VALCGDD176 pKa = 4.85DD177 pKa = 4.26AGEE180 pKa = 4.16DD181 pKa = 3.38QRR183 pKa = 11.84SYY185 pKa = 11.66VGLALGDD192 pKa = 3.9TIVAGDD198 pKa = 4.37NQTEE202 pKa = 4.68DD203 pKa = 4.02YY204 pKa = 11.38DD205 pKa = 5.89DD206 pKa = 6.01DD207 pKa = 6.06DD208 pKa = 6.98DD209 pKa = 6.02EE210 pKa = 6.81IEE212 pKa = 4.54DD213 pKa = 4.19HH214 pKa = 7.43DD215 pKa = 5.37GDD217 pKa = 3.84VDD219 pKa = 4.28GEE221 pKa = 4.28EE222 pKa = 4.24SQHH225 pKa = 7.7ADD227 pKa = 3.28FGDD230 pKa = 3.54MMKK233 pKa = 10.35QYY235 pKa = 11.11AKK237 pKa = 10.52DD238 pKa = 3.49AASEE242 pKa = 4.26TCEE245 pKa = 4.41PVPQEE250 pKa = 3.79LWNTTVRR257 pKa = 11.84DD258 pKa = 5.17GEE260 pKa = 4.25DD261 pKa = 3.39QDD263 pKa = 5.49CKK265 pKa = 11.13GWWQSIAGGARR276 pKa = 11.84SVADD280 pKa = 4.82DD281 pKa = 3.4IGQAFVGGVPLLMEE295 pKa = 5.78DD296 pKa = 3.81GTVADD301 pKa = 5.21CVTSLDD307 pKa = 3.07WDD309 pKa = 3.82YY310 pKa = 12.03DD311 pKa = 3.64FSQNVKK317 pKa = 9.34SACWPSGMEE326 pKa = 4.09EE327 pKa = 4.06MPEE330 pKa = 3.92GNHH333 pKa = 5.62AVRR336 pKa = 11.84VTLEE340 pKa = 4.48LPLKK344 pKa = 10.06CADD347 pKa = 3.55VNGPTMALADD357 pKa = 3.84IPEE360 pKa = 4.13EE361 pKa = 4.09TIVISGEE368 pKa = 4.03VAFCGEE374 pKa = 4.78DD375 pKa = 3.0AGEE378 pKa = 4.19DD379 pKa = 3.41QRR381 pKa = 11.84SYY383 pKa = 10.65RR384 pKa = 11.84WAAGDD389 pKa = 3.31QQ390 pKa = 3.47

Molecular weight: 42.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8MV00|A0A5B8MV00_9CHLO Rad21/Rec8-like protein OS=Chloropicon primus OX=1764295 GN=A3770_12p66340 PE=3 SV=1
MM1 pKa = 6.93GHH3 pKa = 6.11KK4 pKa = 10.15NVWNSHH10 pKa = 4.99PRR12 pKa = 11.84DD13 pKa = 3.78YY14 pKa = 11.68GLGSHH19 pKa = 7.14PCRR22 pKa = 11.84VCGSHH27 pKa = 6.57HH28 pKa = 5.84GVIRR32 pKa = 11.84KK33 pKa = 9.4YY34 pKa = 10.89GLNICRR40 pKa = 11.84RR41 pKa = 11.84CFRR44 pKa = 11.84EE45 pKa = 3.58NAKK48 pKa = 10.78DD49 pKa = 2.91IGFRR53 pKa = 11.84KK54 pKa = 9.62LRR56 pKa = 3.83

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8606

0

8606

4253573

46

11548

494.3

54.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.343 ± 0.028

1.757 ± 0.03

5.416 ± 0.018

7.653 ± 0.039

3.663 ± 0.016

7.856 ± 0.035

2.049 ± 0.013

3.987 ± 0.021

6.411 ± 0.033

9.297 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.012

3.389 ± 0.018

4.274 ± 0.02

3.796 ± 0.017

5.936 ± 0.03

7.929 ± 0.034

4.997 ± 0.023

7.038 ± 0.022

1.216 ± 0.01

2.569 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski