Aeromonas virus 65

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Emmerichvirinae; Ishigurovirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5DS30|E5DS30_9CAUD Uncharacterized protein OS=Aeromonas virus 65 OX=260149 GN=65p196 PE=4 SV=1
MM1 pKa = 7.4QVDD4 pKa = 3.63NYY6 pKa = 11.19LAFLHH11 pKa = 5.88NVKK14 pKa = 10.34GFNFIDD20 pKa = 4.57ASNDD24 pKa = 3.16DD25 pKa = 4.41CLLVHH30 pKa = 7.06LDD32 pKa = 3.27GGKK35 pKa = 10.0VMRR38 pKa = 11.84FEE40 pKa = 4.02MVYY43 pKa = 10.94DD44 pKa = 3.79EE45 pKa = 5.08DD46 pKa = 5.35FEE48 pKa = 4.86MDD50 pKa = 3.27LFMYY54 pKa = 10.51FDD56 pKa = 4.01VEE58 pKa = 4.37TDD60 pKa = 3.32CGLEE64 pKa = 3.92PFDD67 pKa = 5.31INDD70 pKa = 3.22ILTFEE75 pKa = 4.35VV76 pKa = 3.46

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5DRX3|E5DRX3_9CAUD Uncharacterized protein OS=Aeromonas virus 65 OX=260149 GN=65p139 PE=4 SV=1
MM1 pKa = 6.81WRR3 pKa = 11.84SVEE6 pKa = 4.04VTVKK10 pKa = 10.55KK11 pKa = 10.83FEE13 pKa = 4.08EE14 pKa = 3.87SHH16 pKa = 5.98LRR18 pKa = 11.84KK19 pKa = 8.62LTKK22 pKa = 10.36SRR24 pKa = 11.84IKK26 pKa = 10.63AFKK29 pKa = 10.39ASLSPRR35 pKa = 11.84SWYY38 pKa = 10.38AIYY41 pKa = 10.5RR42 pKa = 11.84CLLL45 pKa = 3.59

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

437

0

437

71788

17

1286

164.3

18.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.828 ± 0.145

1.329 ± 0.072

6.854 ± 0.104

6.809 ± 0.164

4.586 ± 0.084

6.273 ± 0.149

2.089 ± 0.07

7.551 ± 0.123

7.439 ± 0.161

6.99 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.23 ± 0.087

5.752 ± 0.105

3.247 ± 0.08

2.961 ± 0.064

4.244 ± 0.079

6.675 ± 0.135

5.685 ± 0.167

7.306 ± 0.1

1.422 ± 0.051

4.728 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski