Elderberry carlavirus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; unclassified Carlavirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A7MDS4|A0A0A7MDS4_9VIRU Triple gene block protein 1 OS=Elderberry carlavirus A OX=1569052 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 4.21VLIKK6 pKa = 10.59LALEE10 pKa = 4.1RR11 pKa = 11.84GFDD14 pKa = 4.26RR15 pKa = 11.84IHH17 pKa = 6.89SNYY20 pKa = 9.77SGCAVFHH27 pKa = 6.19CVPGAGKK34 pKa = 6.92TTLIRR39 pKa = 11.84DD40 pKa = 4.13LLQRR44 pKa = 11.84DD45 pKa = 3.68SRR47 pKa = 11.84FQALTFGVVDD57 pKa = 3.86KK58 pKa = 11.21QSLSGRR64 pKa = 11.84RR65 pKa = 11.84IKK67 pKa = 10.9GPSEE71 pKa = 3.57RR72 pKa = 11.84LEE74 pKa = 4.11EE75 pKa = 4.39GKK77 pKa = 8.59LTIVDD82 pKa = 4.71EE83 pKa = 4.33YY84 pKa = 11.52TEE86 pKa = 4.65GDD88 pKa = 3.23WEE90 pKa = 4.36IYY92 pKa = 10.37KK93 pKa = 10.35PIALFGDD100 pKa = 5.22PIQSCNLSRR109 pKa = 11.84VKK111 pKa = 10.39PSNFEE116 pKa = 3.67CRR118 pKa = 11.84RR119 pKa = 11.84TFRR122 pKa = 11.84FGRR125 pKa = 11.84QTCALLGRR133 pKa = 11.84LGFHH137 pKa = 6.41ITSEE141 pKa = 4.12FDD143 pKa = 3.49DD144 pKa = 4.04EE145 pKa = 5.32VIIGDD150 pKa = 3.76VFSIEE155 pKa = 4.06PEE157 pKa = 3.92GLIIAFEE164 pKa = 4.35PEE166 pKa = 4.19VKK168 pKa = 10.28EE169 pKa = 3.76LLEE172 pKa = 4.62DD173 pKa = 3.76HH174 pKa = 6.63GCDD177 pKa = 3.58YY178 pKa = 11.48SEE180 pKa = 4.48PCALRR185 pKa = 11.84GRR187 pKa = 11.84TTKK190 pKa = 10.73AVTFITASEE199 pKa = 4.62SIPEE203 pKa = 4.14SLAHH207 pKa = 6.62LFFIALTRR215 pKa = 11.84HH216 pKa = 4.23QEE218 pKa = 3.89KK219 pKa = 10.84LIVLSRR225 pKa = 11.84NATFGATT232 pKa = 3.31

Molecular weight:
26.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A7M910|A0A0A7M910_9VIRU Capsid protein OS=Elderberry carlavirus A OX=1569052 PE=3 SV=1
MM1 pKa = 7.84DD2 pKa = 4.79RR3 pKa = 11.84VKK5 pKa = 10.91LMCDD9 pKa = 3.71IIRR12 pKa = 11.84ILSSEE17 pKa = 4.01SRR19 pKa = 11.84EE20 pKa = 3.92AYY22 pKa = 10.41YY23 pKa = 10.13IDD25 pKa = 2.97IARR28 pKa = 11.84YY29 pKa = 8.59IVHH32 pKa = 7.12LSAEE36 pKa = 4.03LHH38 pKa = 6.45APGKK42 pKa = 9.11STFAKK47 pKa = 9.85RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84AKK52 pKa = 10.69SLGRR56 pKa = 11.84CHH58 pKa = 6.31RR59 pKa = 11.84CYY61 pKa = 10.46RR62 pKa = 11.84ISPGFYY68 pKa = 8.89HH69 pKa = 5.52TTKK72 pKa = 10.56CDD74 pKa = 4.11GISCTDD80 pKa = 3.2SFGSRR85 pKa = 11.84SKK87 pKa = 9.68HH88 pKa = 5.23RR89 pKa = 11.84NYY91 pKa = 9.76IVHH94 pKa = 6.51GQRR97 pKa = 11.84KK98 pKa = 7.88HH99 pKa = 5.07CC100 pKa = 4.36

Molecular weight:
11.62 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2807

72

1995

467.8

52.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.27 ± 0.587

3.206 ± 0.389

4.881 ± 0.345

6.947 ± 1.084

5.522 ± 0.854

6.056 ± 0.507

2.458 ± 0.706

5.949 ± 0.555

6.484 ± 0.599

10.046 ± 1.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.031 ± 0.336

4.239 ± 0.558

4.275 ± 0.628

2.814 ± 0.462

5.878 ± 0.985

7.731 ± 0.685

5.166 ± 0.555

5.771 ± 0.497

0.891 ± 0.184

3.384 ± 0.662

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski