Amino acid dipepetide frequency for Novosphingobium nitrogenifigens DSM 19370

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.186AlaAla: 18.186 ± 0.173	1.161AlaCys: 1.161 ± 0.037	7.534AlaAsp: 7.534 ± 0.089	7.162AlaGlu: 7.162 ± 0.084	4.372AlaPhe: 4.372 ± 0.061	11.632AlaGly: 11.632 ± 0.099	2.921AlaHis: 2.921 ± 0.059	6.618AlaIle: 6.618 ± 0.065	3.44AlaLys: 3.44 ± 0.062	14.79AlaLeu: 14.79 ± 0.131	4.122AlaMet: 4.122 ± 0.062	3.014AlaAsn: 3.014 ± 0.052	6.585AlaPro: 6.585 ± 0.094	4.726AlaGln: 4.726 ± 0.065	10.523AlaArg: 10.523 ± 0.117	6.678AlaSer: 6.678 ± 0.079	6.767AlaThr: 6.767 ± 0.075	8.777AlaVal: 8.777 ± 0.095	1.674AlaTrp: 1.674 ± 0.039	2.592AlaTyr: 2.592 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.069CysAla: 1.069 ± 0.031	0.108CysCys: 0.108 ± 0.01	0.538CysAsp: 0.538 ± 0.018	0.422CysGlu: 0.422 ± 0.018	0.297CysPhe: 0.297 ± 0.016	0.972CysGly: 0.972 ± 0.031	0.269CysHis: 0.269 ± 0.019	0.366CysIle: 0.366 ± 0.019	0.177CysLys: 0.177 ± 0.013	0.78CysLeu: 0.78 ± 0.028	0.159CysMet: 0.159 ± 0.012	0.213CysAsn: 0.213 ± 0.015	0.528CysPro: 0.528 ± 0.021	0.213CysGln: 0.213 ± 0.014	0.653CysArg: 0.653 ± 0.027	0.451CysSer: 0.451 ± 0.02	0.44CysThr: 0.44 ± 0.02	0.558CysVal: 0.558 ± 0.023	0.125CysTrp: 0.125 ± 0.011	0.195CysTyr: 0.195 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.3AspAla: 7.3 ± 0.081	0.515AspCys: 0.515 ± 0.023	3.046AspAsp: 3.046 ± 0.056	3.26AspGlu: 3.26 ± 0.056	2.194AspPhe: 2.194 ± 0.048	5.175AspGly: 5.175 ± 0.073	1.588AspHis: 1.588 ± 0.032	2.74AspIle: 2.74 ± 0.046	1.688AspLys: 1.688 ± 0.034	6.039AspLeu: 6.039 ± 0.077	1.388AspMet: 1.388 ± 0.03	1.332AspAsn: 1.332 ± 0.035	3.873AspPro: 3.873 ± 0.06	1.597AspGln: 1.597 ± 0.037	4.683AspArg: 4.683 ± 0.068	2.153AspSer: 2.153 ± 0.044	2.735AspThr: 2.735 ± 0.047	4.004AspVal: 4.004 ± 0.058	1.136AspTrp: 1.136 ± 0.028	1.602AspTyr: 1.602 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	7.64GluAla: 7.64 ± 0.088	0.381GluCys: 0.381 ± 0.018	2.673GluAsp: 2.673 ± 0.056	2.783GluGlu: 2.783 ± 0.057	1.528GluPhe: 1.528 ± 0.039	4.407GluGly: 4.407 ± 0.066	1.129GluHis: 1.129 ± 0.031	2.879GluIle: 2.879 ± 0.05	1.666GluLys: 1.666 ± 0.039	4.609GluLeu: 4.609 ± 0.058	1.267GluMet: 1.267 ± 0.033	1.321GluAsn: 1.321 ± 0.036	2.53GluPro: 2.53 ± 0.05	1.841GluGln: 1.841 ± 0.04	4.795GluArg: 4.795 ± 0.079	2.048GluSer: 2.048 ± 0.038	3.321GluThr: 3.321 ± 0.06	3.491GluVal: 3.491 ± 0.056	0.782GluTrp: 0.782 ± 0.025	0.861GluTyr: 0.861 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	5.011PheAla: 5.011 ± 0.073	0.327PheCys: 0.327 ± 0.018	2.58PheAsp: 2.58 ± 0.052	1.81PheGlu: 1.81 ± 0.036	1.236PhePhe: 1.236 ± 0.038	3.666PheGly: 3.666 ± 0.053	0.828PheHis: 0.828 ± 0.027	1.409PheIle: 1.409 ± 0.035	0.714PheLys: 0.714 ± 0.025	3.084PheLeu: 3.084 ± 0.056	0.717PheMet: 0.717 ± 0.027	1.028PheAsn: 1.028 ± 0.033	1.598PhePro: 1.598 ± 0.038	0.853PheGln: 0.853 ± 0.027	2.189PheArg: 2.189 ± 0.042	2.049PheSer: 2.049 ± 0.042	2.055PheThr: 2.055 ± 0.042	2.592PheVal: 2.592 ± 0.053	0.507PheTrp: 0.507 ± 0.023	0.897PheTyr: 0.897 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.039GlyAla: 10.039 ± 0.099	0.863GlyCys: 0.863 ± 0.027	4.713GlyAsp: 4.713 ± 0.064	4.947GlyGlu: 4.947 ± 0.066	3.649GlyPhe: 3.649 ± 0.058	8.057GlyGly: 8.057 ± 0.106	2.278GlyHis: 2.278 ± 0.045	4.569GlyIle: 4.569 ± 0.061	3.301GlyLys: 3.301 ± 0.052	9.15GlyLeu: 9.15 ± 0.087	2.419GlyMet: 2.419 ± 0.048	2.352GlyAsn: 2.352 ± 0.051	3.86GlyPro: 3.86 ± 0.062	3.117GlyGln: 3.117 ± 0.053	6.301GlyArg: 6.301 ± 0.081	4.909GlySer: 4.909 ± 0.07	5.211GlyThr: 5.211 ± 0.07	6.288GlyVal: 6.288 ± 0.078	1.653GlyTrp: 1.653 ± 0.037	2.351GlyTyr: 2.351 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.941HisAla: 2.941 ± 0.054	0.261HisCys: 0.261 ± 0.014	1.497HisAsp: 1.497 ± 0.036	1.132HisGlu: 1.132 ± 0.039	0.938HisPhe: 0.938 ± 0.029	2.325HisGly: 2.325 ± 0.049	0.67HisHis: 0.67 ± 0.028	0.962HisIle: 0.962 ± 0.025	0.493HisLys: 0.493 ± 0.023	2.191HisLeu: 2.191 ± 0.045	0.489HisMet: 0.489 ± 0.022	0.496HisAsn: 0.496 ± 0.022	1.518HisPro: 1.518 ± 0.038	0.572HisGln: 0.572 ± 0.023	1.712HisArg: 1.712 ± 0.041	1.009HisSer: 1.009 ± 0.029	0.877HisThr: 0.877 ± 0.025	1.838HisVal: 1.838 ± 0.04	0.388HisTrp: 0.388 ± 0.018	0.682HisTyr: 0.682 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.807IleAla: 7.807 ± 0.088	0.449IleCys: 0.449 ± 0.019	3.832IleAsp: 3.832 ± 0.054	3.284IleGlu: 3.284 ± 0.06	1.376IlePhe: 1.376 ± 0.037	4.898IleGly: 4.898 ± 0.053	0.934IleHis: 0.934 ± 0.027	1.697IleIle: 1.697 ± 0.04	1.148IleLys: 1.148 ± 0.034	3.762IleLeu: 3.762 ± 0.056	0.899IleMet: 0.899 ± 0.028	1.35IleAsn: 1.35 ± 0.035	2.316IlePro: 2.316 ± 0.04	1.041IleGln: 1.041 ± 0.032	3.112IleArg: 3.112 ± 0.049	2.524IleSer: 2.524 ± 0.052	2.602IleThr: 2.602 ± 0.045	4.036IleVal: 4.036 ± 0.058	0.556IleTrp: 0.556 ± 0.024	1.015IleTyr: 1.015 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.909LysAla: 3.909 ± 0.065	0.138LysCys: 0.138 ± 0.01	1.377LysAsp: 1.377 ± 0.037	1.06LysGlu: 1.06 ± 0.033	0.723LysPhe: 0.723 ± 0.025	2.629LysGly: 2.629 ± 0.046	0.462LysHis: 0.462 ± 0.02	1.267LysIle: 1.267 ± 0.034	0.817LysLys: 0.817 ± 0.033	2.749LysLeu: 2.749 ± 0.046	0.597LysMet: 0.597 ± 0.023	0.604LysAsn: 0.604 ± 0.023	1.765LysPro: 1.765 ± 0.04	0.777LysGln: 0.777 ± 0.024	1.776LysArg: 1.776 ± 0.037	1.4LysSer: 1.4 ± 0.033	1.605LysThr: 1.605 ± 0.041	2.239LysVal: 2.239 ± 0.043	0.338LysTrp: 0.338 ± 0.02	0.551LysTyr: 0.551 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	15.659LeuAla: 15.659 ± 0.136	0.875LeuCys: 0.875 ± 0.032	6.243LeuAsp: 6.243 ± 0.083	4.53LeuGlu: 4.53 ± 0.067	3.502LeuPhe: 3.502 ± 0.069	8.855LeuGly: 8.855 ± 0.093	2.16LeuHis: 2.16 ± 0.037	4.572LeuIle: 4.572 ± 0.06	2.636LeuLys: 2.636 ± 0.04	9.027LeuLeu: 9.027 ± 0.117	2.018LeuMet: 2.018 ± 0.048	2.17LeuAsn: 2.17 ± 0.042	5.96LeuPro: 5.96 ± 0.072	2.361LeuGln: 2.361 ± 0.047	7.186LeuArg: 7.186 ± 0.093	6.15LeuSer: 6.15 ± 0.084	5.428LeuThr: 5.428 ± 0.064	8.09LeuVal: 8.09 ± 0.089	1.221LeuTrp: 1.221 ± 0.033	1.899LeuTyr: 1.899 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.463MetAla: 3.463 ± 0.053	0.154MetCys: 0.154 ± 0.013	1.03MetAsp: 1.03 ± 0.029	0.966MetGlu: 0.966 ± 0.033	0.639MetPhe: 0.639 ± 0.025	1.93MetGly: 1.93 ± 0.04	0.476MetHis: 0.476 ± 0.021	1.363MetIle: 1.363 ± 0.036	0.789MetLys: 0.789 ± 0.026	2.549MetLeu: 2.549 ± 0.05	0.64MetMet: 0.64 ± 0.028	0.635MetAsn: 0.635 ± 0.022	1.471MetPro: 1.471 ± 0.032	0.735MetGln: 0.735 ± 0.024	1.701MetArg: 1.701 ± 0.041	1.386MetSer: 1.386 ± 0.034	1.914MetThr: 1.914 ± 0.039	1.826MetVal: 1.826 ± 0.042	0.213MetTrp: 0.213 ± 0.014	0.244MetTyr: 0.244 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.243AsnAla: 3.243 ± 0.069	0.246AsnCys: 0.246 ± 0.015	1.333AsnAsp: 1.333 ± 0.031	1.029AsnGlu: 1.029 ± 0.027	0.894AsnPhe: 0.894 ± 0.029	2.399AsnGly: 2.399 ± 0.045	0.493AsnHis: 0.493 ± 0.02	1.118AsnIle: 1.118 ± 0.04	0.57AsnLys: 0.57 ± 0.019	2.455AsnLeu: 2.455 ± 0.06	0.493AsnMet: 0.493 ± 0.021	0.683AsnAsn: 0.683 ± 0.028	1.799AsnPro: 1.799 ± 0.038	0.711AsnGln: 0.711 ± 0.026	1.792AsnArg: 1.792 ± 0.036	1.223AsnSer: 1.223 ± 0.037	1.254AsnThr: 1.254 ± 0.04	1.808AsnVal: 1.808 ± 0.044	0.386AsnTrp: 0.386 ± 0.02	0.666AsnTyr: 0.666 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	7.217ProAla: 7.217 ± 0.102	0.403ProCys: 0.403 ± 0.019	3.912ProAsp: 3.912 ± 0.053	3.518ProGlu: 3.518 ± 0.056	1.972ProPhe: 1.972 ± 0.038	5.148ProGly: 5.148 ± 0.07	1.23ProHis: 1.23 ± 0.03	2.458ProIle: 2.458 ± 0.041	1.269ProLys: 1.269 ± 0.037	5.268ProLeu: 5.268 ± 0.062	1.277ProMet: 1.277 ± 0.037	1.109ProAsn: 1.109 ± 0.035	2.719ProPro: 2.719 ± 0.068	1.804ProGln: 1.804 ± 0.034	3.29ProArg: 3.29 ± 0.058	2.822ProSer: 2.822 ± 0.054	2.685ProThr: 2.685 ± 0.044	4.711ProVal: 4.711 ± 0.071	0.738ProTrp: 0.738 ± 0.028	1.085ProTyr: 1.085 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.316GlnAla: 4.316 ± 0.069	0.223GlnCys: 0.223 ± 0.014	1.412GlnAsp: 1.412 ± 0.035	1.194GlnGlu: 1.194 ± 0.031	1.087GlnPhe: 1.087 ± 0.031	2.689GlnGly: 2.689 ± 0.045	0.636GlnHis: 0.636 ± 0.021	1.666GlnIle: 1.666 ± 0.038	0.872GlnLys: 0.872 ± 0.031	2.719GlnLeu: 2.719 ± 0.048	0.772GlnMet: 0.772 ± 0.027	0.731GlnAsn: 0.731 ± 0.029	1.721GlnPro: 1.721 ± 0.043	1.153GlnGln: 1.153 ± 0.04	2.267GlnArg: 2.267 ± 0.041	1.704GlnSer: 1.704 ± 0.039	1.685GlnThr: 1.685 ± 0.041	2.426GlnVal: 2.426 ± 0.047	0.492GlnTrp: 0.492 ± 0.018	0.662GlnTyr: 0.662 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	8.844ArgAla: 8.844 ± 0.101	0.513ArgCys: 0.513 ± 0.023	4.13ArgAsp: 4.13 ± 0.058	4.097ArgGlu: 4.097 ± 0.069	3.112ArgPhe: 3.112 ± 0.05	5.1ArgGly: 5.1 ± 0.071	2.115ArgHis: 2.115 ± 0.043	4.117ArgIle: 4.117 ± 0.067	2.102ArgLys: 2.102 ± 0.042	8.583ArgLeu: 8.583 ± 0.094	1.921ArgMet: 1.921 ± 0.044	1.843ArgAsn: 1.843 ± 0.041	3.746ArgPro: 3.746 ± 0.056	2.468ArgGln: 2.468 ± 0.05	5.91ArgArg: 5.91 ± 0.081	3.477ArgSer: 3.477 ± 0.055	3.654ArgThr: 3.654 ± 0.053	4.972ArgVal: 4.972 ± 0.064	1.178ArgTrp: 1.178 ± 0.032	1.743ArgTyr: 1.743 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.415SerAla: 6.415 ± 0.081	0.451SerCys: 0.451 ± 0.021	2.883SerAsp: 2.883 ± 0.048	2.437SerGlu: 2.437 ± 0.044	2.023SerPhe: 2.023 ± 0.039	5.536SerGly: 5.536 ± 0.076	1.114SerHis: 1.114 ± 0.028	2.47SerIle: 2.47 ± 0.052	1.131SerLys: 1.131 ± 0.036	5.382SerLeu: 5.382 ± 0.074	1.127SerMet: 1.127 ± 0.028	1.405SerAsn: 1.405 ± 0.037	2.996SerPro: 2.996 ± 0.052	1.558SerGln: 1.558 ± 0.038	3.51SerArg: 3.51 ± 0.059	2.989SerSer: 2.989 ± 0.072	2.794SerThr: 2.794 ± 0.06	3.663SerVal: 3.663 ± 0.07	0.766SerTrp: 0.766 ± 0.026	1.324SerTyr: 1.324 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	6.42ThrAla: 6.42 ± 0.079	0.498ThrCys: 0.498 ± 0.02	2.89ThrAsp: 2.89 ± 0.056	2.228ThrGlu: 2.228 ± 0.046	1.878ThrPhe: 1.878 ± 0.039	5.648ThrGly: 5.648 ± 0.07	1.131ThrHis: 1.131 ± 0.029	3.02ThrIle: 3.02 ± 0.055	1.132ThrLys: 1.132 ± 0.036	5.969ThrLeu: 5.969 ± 0.083	1.327ThrMet: 1.327 ± 0.04	1.32ThrAsn: 1.32 ± 0.04	3.506ThrPro: 3.506 ± 0.064	1.541ThrGln: 1.541 ± 0.035	3.645ThrArg: 3.645 ± 0.055	2.904ThrSer: 2.904 ± 0.065	3.102ThrThr: 3.102 ± 0.065	4.338ThrVal: 4.338 ± 0.071	0.704ThrTrp: 0.704 ± 0.027	1.268ThrTyr: 1.268 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	9.752ValAla: 9.752 ± 0.1	0.605ValCys: 0.605 ± 0.021	4.228ValAsp: 4.228 ± 0.049	4.457ValGlu: 4.457 ± 0.074	2.36ValPhe: 2.36 ± 0.045	5.809ValGly: 5.809 ± 0.072	1.543ValHis: 1.543 ± 0.035	3.805ValIle: 3.805 ± 0.056	1.875ValLys: 1.875 ± 0.039	7.583ValLeu: 7.583 ± 0.088	1.748ValMet: 1.748 ± 0.036	1.891ValAsn: 1.891 ± 0.041	4.355ValPro: 4.355 ± 0.066	1.909ValGln: 1.909 ± 0.036	5.294ValArg: 5.294 ± 0.073	4.084ValSer: 4.084 ± 0.063	4.434ValThr: 4.434 ± 0.066	5.757ValVal: 5.757 ± 0.084	0.921ValTrp: 0.921 ± 0.029	1.395ValTyr: 1.395 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.347TrpAla: 1.347 ± 0.034	0.131TrpCys: 0.131 ± 0.011	0.705TrpAsp: 0.705 ± 0.027	0.558TrpGlu: 0.558 ± 0.02	0.572TrpPhe: 0.572 ± 0.023	0.971TrpGly: 0.971 ± 0.027	0.455TrpHis: 0.455 ± 0.019	0.709TrpIle: 0.709 ± 0.026	0.448TrpLys: 0.448 ± 0.02	1.812TrpLeu: 1.812 ± 0.046	0.35TrpMet: 0.35 ± 0.018	0.439TrpAsn: 0.439 ± 0.018	0.739TrpPro: 0.739 ± 0.029	0.698TrpGln: 0.698 ± 0.024	1.376TrpArg: 1.376 ± 0.036	0.876TrpSer: 0.876 ± 0.025	0.785TrpThr: 0.785 ± 0.026	0.833TrpVal: 0.833 ± 0.023	0.266TrpTrp: 0.266 ± 0.017	0.318TrpTyr: 0.318 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.696TyrAla: 2.696 ± 0.049	0.231TyrCys: 0.231 ± 0.014	1.492TyrAsp: 1.492 ± 0.04	1.076TyrGlu: 1.076 ± 0.032	0.8TyrPhe: 0.8 ± 0.026	2.183TyrGly: 2.183 ± 0.047	0.556TyrHis: 0.556 ± 0.022	0.833TyrIle: 0.833 ± 0.029	0.515TyrLys: 0.515 ± 0.022	2.041TyrLeu: 2.041 ± 0.04	0.39TyrMet: 0.39 ± 0.016	0.662TyrAsn: 0.662 ± 0.03	1.052TyrPro: 1.052 ± 0.032	0.749TyrGln: 0.749 ± 0.028	1.913TyrArg: 1.913 ± 0.044	1.138TyrSer: 1.138 ± 0.032	1.122TyrThr: 1.122 ± 0.038	1.559TyrVal: 1.559 ± 0.043	0.343TyrTrp: 0.343 ± 0.016	0.666TyrTyr: 0.666 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3800 proteins (1232021 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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