Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharopolyspora; Saccharopolyspora erythraea

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A4FPJ2|RPOA_SACEN DNA-directed RNA polymerase subunit alpha OS=Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) OX=405948 GN=rpoA PE=3 SV=1
MM1 pKa = 7.49PHH3 pKa = 7.34DD4 pKa = 5.25PLPPDD9 pKa = 4.65PFAGDD14 pKa = 3.76PDD16 pKa = 4.62DD17 pKa = 5.12PSMTLGDD24 pKa = 4.53HH25 pKa = 7.08DD26 pKa = 5.15PEE28 pKa = 4.88FGPPLGDD35 pKa = 3.42EE36 pKa = 4.41EE37 pKa = 4.36QAEE40 pKa = 4.38LLADD44 pKa = 3.81LTDD47 pKa = 4.08LSVYY51 pKa = 9.8QALLEE56 pKa = 4.1PRR58 pKa = 11.84GVRR61 pKa = 11.84GIVVDD66 pKa = 4.1CTDD69 pKa = 3.94CGEE72 pKa = 4.25SHH74 pKa = 5.46YY75 pKa = 11.01HH76 pKa = 6.18DD77 pKa = 4.24WEE79 pKa = 4.1LLRR82 pKa = 11.84ASLEE86 pKa = 3.79QLLNDD91 pKa = 3.31GRR93 pKa = 11.84MRR95 pKa = 11.84PHH97 pKa = 6.9EE98 pKa = 4.35PAFDD102 pKa = 4.7PNPEE106 pKa = 4.03QYY108 pKa = 8.85VTWEE112 pKa = 4.04YY113 pKa = 11.38CRR115 pKa = 11.84GFADD119 pKa = 4.29GVTEE123 pKa = 4.1AEE125 pKa = 4.56NEE127 pKa = 3.98NSHH130 pKa = 6.62

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4F5V1|A4F5V1_SACEN Macrolide 2'-phosphotransferase OS=Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) OX=405948 GN=mphB PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.65HH19 pKa = 4.47GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84GRR42 pKa = 11.84ASLTAA47 pKa = 4.2

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7154

0

7154

2307965

30

11792

322.6

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.147 ± 0.045

0.828 ± 0.01

6.011 ± 0.022

6.103 ± 0.03

2.866 ± 0.019

9.44 ± 0.031

2.356 ± 0.017

3.156 ± 0.024

1.759 ± 0.019

10.357 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.013

1.777 ± 0.016

5.912 ± 0.026

2.867 ± 0.019

8.394 ± 0.033

5.283 ± 0.023

5.508 ± 0.017

9.02 ± 0.032

1.522 ± 0.012

1.888 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski