Cellulophaga phage phi18:3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 123 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A2B7|S0A2B7_9CAUD Uncharacterized protein OS=Cellulophaga phage phi18:3 OX=1327983 GN=Phi18:3_gp095 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.61NKK4 pKa = 8.56ITIIMILMSISFGYY18 pKa = 9.85SQVLVPEE25 pKa = 4.2NTGYY29 pKa = 11.18GYY31 pKa = 11.07DD32 pKa = 3.53NLTTVQLNALTKK44 pKa = 10.45KK45 pKa = 10.43KK46 pKa = 10.94VGDD49 pKa = 3.23IYY51 pKa = 11.59YY52 pKa = 10.48NADD55 pKa = 3.03LGYY58 pKa = 9.03HH59 pKa = 5.2VKK61 pKa = 9.49WNGVIFEE68 pKa = 4.6SLASGISGIDD78 pKa = 3.43PADD81 pKa = 3.37QDD83 pKa = 3.85KK84 pKa = 11.33LNNISITQPVNLDD97 pKa = 3.69TVEE100 pKa = 4.31SLANSALQTEE110 pKa = 4.96TDD112 pKa = 3.69PTTTPANIKK121 pKa = 10.45AKK123 pKa = 10.55LEE125 pKa = 4.19SLTGTNRR132 pKa = 11.84LDD134 pKa = 3.27AFAIKK139 pKa = 10.27NLSAQEE145 pKa = 3.96TDD147 pKa = 3.46PNLTKK152 pKa = 11.0SNVEE156 pKa = 3.97GLGISYY162 pKa = 9.47TSLSDD167 pKa = 3.67VPSVSPPLTFGPGFTRR183 pKa = 11.84TGDD186 pKa = 3.71NIVLDD191 pKa = 4.09NPFTTANEE199 pKa = 4.21TTLNSALQSEE209 pKa = 4.65TDD211 pKa = 3.7PLYY214 pKa = 10.96SAWDD218 pKa = 3.41KK219 pKa = 11.41DD220 pKa = 4.07YY221 pKa = 11.58NDD223 pKa = 5.36LINRR227 pKa = 11.84PVNLVTYY234 pKa = 10.27DD235 pKa = 3.75PLTAQDD241 pKa = 3.55VQISVLGGSDD251 pKa = 3.46SEE253 pKa = 4.81SYY255 pKa = 10.68ILEE258 pKa = 4.39FDD260 pKa = 3.74PDD262 pKa = 3.92GYY264 pKa = 10.94DD265 pKa = 3.42YY266 pKa = 11.44SGEE269 pKa = 3.95PSGTGTVSQTIIDD282 pKa = 4.05GDD284 pKa = 4.09TNAVSGNAVFDD295 pKa = 3.89GLATKK300 pKa = 10.28LQAGSNDD307 pKa = 3.43LGSSNFYY314 pKa = 10.92VNSDD318 pKa = 4.2GILALANSNTQSTVFVNPYY337 pKa = 10.2GSGLIAGNSDD347 pKa = 3.19DD348 pKa = 4.6SEE350 pKa = 4.28IMKK353 pKa = 9.18LTLSPSSITADD364 pKa = 3.66LSNSQITSTGATSLITKK381 pKa = 9.4GYY383 pKa = 10.9ADD385 pKa = 3.54ATYY388 pKa = 10.93ASSNQNLSLDD398 pKa = 3.5MGYY401 pKa = 11.01GILSISGGNSVDD413 pKa = 4.2LSATSSYY420 pKa = 11.09TSFDD424 pKa = 3.19IVDD427 pKa = 3.77TGSGATYY434 pKa = 10.36AKK436 pKa = 10.41SGVQEE441 pKa = 3.92KK442 pKa = 11.07DD443 pKa = 3.43LITEE447 pKa = 4.54LSQNNIILSSEE458 pKa = 4.23TNTVSGTPSGNLRR471 pKa = 11.84ILLQTYY477 pKa = 7.76YY478 pKa = 10.95VQTEE482 pKa = 4.44FKK484 pKa = 10.26TFFDD488 pKa = 4.03VFIEE492 pKa = 4.0NMNGVGDD499 pKa = 4.14YY500 pKa = 10.0YY501 pKa = 11.41LKK503 pKa = 11.08GILNPAGTQIDD514 pKa = 4.71VYY516 pKa = 11.19SVGSDD521 pKa = 3.21GLLTPVSAPTSLGYY535 pKa = 9.93IQITGTYY542 pKa = 8.97FYY544 pKa = 11.31AGFF547 pKa = 4.16

Molecular weight: 58.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9ZZ49|R9ZZ49_9CAUD Uncharacterized protein OS=Cellulophaga phage phi18:3 OX=1327983 GN=Phi18:3_gp107 PE=4 SV=1
MM1 pKa = 6.87EE2 pKa = 4.61QKK4 pKa = 10.63NPSRR8 pKa = 11.84LGRR11 pKa = 11.84VLNITGRR18 pKa = 11.84YY19 pKa = 8.75IEE21 pKa = 4.72LKK23 pKa = 10.36KK24 pKa = 11.02ASTKK28 pKa = 11.02DD29 pKa = 3.54NINIRR34 pKa = 11.84YY35 pKa = 9.18ISS37 pKa = 3.34

Molecular weight: 4.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

123

0

123

22253

33

1935

180.9

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.087 ± 0.248

0.966 ± 0.124

6.453 ± 0.193

7.891 ± 0.302

4.624 ± 0.199

5.81 ± 0.285

1.294 ± 0.167

7.878 ± 0.218

9.338 ± 0.462

8.089 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.166

6.817 ± 0.311

2.741 ± 0.136

3.114 ± 0.206

3.685 ± 0.199

6.925 ± 0.236

6.228 ± 0.312

5.635 ± 0.169

1.025 ± 0.097

4.107 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski