Duganella sp. Root198D2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Duganella; unclassified Duganella

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8RD05|A0A0Q8RD05_9BURK CENP-V/GFA domain-containing protein OS=Duganella sp. Root198D2 OX=1736489 GN=ASE26_26435 PE=3 SV=1
MM1 pKa = 6.89TAVAEE6 pKa = 4.3DD7 pKa = 4.6VIPAPIVFTDD17 pKa = 3.96AAAEE21 pKa = 4.14KK22 pKa = 9.63VAQLIEE28 pKa = 4.38EE29 pKa = 4.51EE30 pKa = 4.63GNPDD34 pKa = 2.85LKK36 pKa = 11.13LRR38 pKa = 11.84VFVQGGGCSGFQYY51 pKa = 10.94GFTFDD56 pKa = 4.69EE57 pKa = 4.93IVNEE61 pKa = 4.83DD62 pKa = 3.85DD63 pKa = 3.11TTMVKK68 pKa = 10.58NGVQLLIDD76 pKa = 3.77SMSYY80 pKa = 10.23QYY82 pKa = 11.55LVGAEE87 pKa = 4.01IDD89 pKa = 3.89YY90 pKa = 11.28KK91 pKa = 11.39DD92 pKa = 4.47DD93 pKa = 4.08LEE95 pKa = 4.56GAQFVIKK102 pKa = 10.51NPSATSTCGCGSSFSVV118 pKa = 3.54

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8QS59|A0A0Q8QS59_9BURK DUF2249 domain-containing protein OS=Duganella sp. Root198D2 OX=1736489 GN=ASE26_07250 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.63MKK5 pKa = 9.83TKK7 pKa = 10.72SSAKK11 pKa = 9.15KK12 pKa = 9.67RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.54SGMAFKK29 pKa = 10.69RR30 pKa = 11.84HH31 pKa = 5.84ILTKK35 pKa = 9.78KK36 pKa = 3.98TTKK39 pKa = 10.23NKK41 pKa = 9.21RR42 pKa = 11.84QLRR45 pKa = 11.84GTRR48 pKa = 11.84NINASDD54 pKa = 3.65VTSVLRR60 pKa = 11.84MMPTAA65 pKa = 4.6

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5756

0

5756

1964093

27

6379

341.2

37.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.589 ± 0.044

0.872 ± 0.012

5.257 ± 0.024

5.426 ± 0.034

3.607 ± 0.02

8.261 ± 0.035

2.155 ± 0.017

4.478 ± 0.021

3.819 ± 0.031

10.434 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.016

3.15 ± 0.03

4.907 ± 0.02

4.126 ± 0.023

6.41 ± 0.033

5.728 ± 0.03

4.975 ± 0.034

7.159 ± 0.03

1.416 ± 0.015

2.606 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski