Passion fruit chlorotic mottle virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Citlodavirus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4Q8V4|A0A2R4Q8V4_9GEMI Replication-associated protein OS=Passion fruit chlorotic mottle virus OX=2162638 PE=3 SV=1
MM1 pKa = 7.52SISSEE6 pKa = 3.5IFEE9 pKa = 4.66EE10 pKa = 3.98IKK12 pKa = 10.46EE13 pKa = 4.25NKK15 pKa = 9.73VSITVFFIIFFCVSVINVVCCAFGFWQSAGPRR47 pKa = 11.84GGAVVYY53 pKa = 10.82VGGEE57 pKa = 4.09AINDD61 pKa = 3.89GGEE64 pKa = 4.3EE65 pKa = 3.84ICSGKK70 pKa = 10.06KK71 pKa = 9.6AQPGG75 pKa = 3.34

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4Q8V2|A0A2R4Q8V2_9GEMI Movement protein BC1 OS=Passion fruit chlorotic mottle virus OX=2162638 PE=3 SV=1
MM1 pKa = 6.44VTTRR5 pKa = 11.84SGRR8 pKa = 11.84TYY10 pKa = 10.96GPPTAQTRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84FPPMRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84PAMDD31 pKa = 3.42RR32 pKa = 11.84VIGPSKK38 pKa = 9.92KK39 pKa = 7.95TVKK42 pKa = 10.17RR43 pKa = 11.84MKK45 pKa = 9.71KK46 pKa = 9.09GKK48 pKa = 10.12SGGIPVGCKK57 pKa = 10.25GPCKK61 pKa = 9.43THH63 pKa = 5.36TVDD66 pKa = 4.55VIATVTHH73 pKa = 6.94DD74 pKa = 3.49GKK76 pKa = 11.54GPGLVSNISKK86 pKa = 10.87GDD88 pKa = 3.58DD89 pKa = 3.1FGQRR93 pKa = 11.84EE94 pKa = 4.14GRR96 pKa = 11.84RR97 pKa = 11.84IRR99 pKa = 11.84VTKK102 pKa = 10.48LLLRR106 pKa = 11.84GKK108 pKa = 9.77VWLPQDD114 pKa = 3.25KK115 pKa = 9.57ATIAGSNIMRR125 pKa = 11.84LWVMKK130 pKa = 10.18DD131 pKa = 3.08RR132 pKa = 11.84RR133 pKa = 11.84PGSQHH138 pKa = 4.92VAFEE142 pKa = 4.47ALFDD146 pKa = 3.97MADD149 pKa = 4.06KK150 pKa = 10.68EE151 pKa = 4.29PSTALVKK158 pKa = 9.75MDD160 pKa = 3.2YY161 pKa = 10.03RR162 pKa = 11.84DD163 pKa = 3.33RR164 pKa = 11.84FIVIKK169 pKa = 10.75DD170 pKa = 3.48MEE172 pKa = 6.08IDD174 pKa = 3.4LHH176 pKa = 6.29GGRR179 pKa = 11.84DD180 pKa = 3.71FRR182 pKa = 11.84VDD184 pKa = 3.43EE185 pKa = 4.26EE186 pKa = 4.73TFDD189 pKa = 3.27IMVPINCDD197 pKa = 3.07VLFDD201 pKa = 4.75HH202 pKa = 7.1NDD204 pKa = 3.44EE205 pKa = 4.8GSLTTTLEE213 pKa = 3.72NGIIVYY219 pKa = 9.53YY220 pKa = 10.78AVTDD224 pKa = 4.07PAQVMQLTAQCRR236 pKa = 11.84LYY238 pKa = 11.08FFDD241 pKa = 3.86STSNN245 pKa = 3.26

Molecular weight: 27.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1335

75

319

222.5

25.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.318 ± 0.604

2.397 ± 0.279

7.116 ± 0.713

5.169 ± 0.649

6.367 ± 0.983

4.944 ± 1.263

2.996 ± 0.352

6.891 ± 0.599

5.693 ± 0.914

5.843 ± 0.723

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.221 ± 0.348

4.419 ± 0.48

6.067 ± 0.767

2.472 ± 0.319

7.865 ± 0.973

7.566 ± 1.001

5.019 ± 0.929

5.993 ± 1.053

1.723 ± 0.363

2.921 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski