Vibrio phage Rostov 7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W3G6|A0A481W3G6_9CAUD Uncharacterized protein OS=Vibrio phage Rostov 7 OX=2544946 GN=Rostov7_00045 PE=4 SV=1
MM1 pKa = 8.13PFRR4 pKa = 11.84QGTTDD9 pKa = 3.74CNWFKK14 pKa = 10.86KK15 pKa = 10.39VYY17 pKa = 10.74SLDD20 pKa = 3.64EE21 pKa = 4.55VINNPLSKK29 pKa = 10.69LSDD32 pKa = 3.27VHH34 pKa = 7.01HH35 pKa = 5.78QAEE38 pKa = 4.66LVATRR43 pKa = 11.84QWTGLKK49 pKa = 10.21DD50 pKa = 3.55KK51 pKa = 11.04QGVDD55 pKa = 2.91IFEE58 pKa = 4.45GDD60 pKa = 3.15ILQYY64 pKa = 11.08NCGDD68 pKa = 3.55GEE70 pKa = 4.53EE71 pKa = 4.51YY72 pKa = 11.15NLGQVEE78 pKa = 4.64WFKK81 pKa = 10.87PWNGWCVMTSGSIPDD96 pKa = 3.54AFGDD100 pKa = 4.18RR101 pKa = 11.84EE102 pKa = 4.55STTADD107 pKa = 3.37YY108 pKa = 9.99WCDD111 pKa = 3.26EE112 pKa = 4.36LLVIGNIYY120 pKa = 10.13QNPTWKK126 pKa = 10.61

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W462|A0A481W462_9CAUD Tail sheath protein OS=Vibrio phage Rostov 7 OX=2544946 GN=Rostov7_00119 PE=4 SV=1
MM1 pKa = 7.28NIWRR5 pKa = 11.84GKK7 pKa = 10.12DD8 pKa = 3.04LAVINWQNGEE18 pKa = 3.84QLMKK22 pKa = 10.37KK23 pKa = 10.18KK24 pKa = 10.77SFTRR28 pKa = 11.84EE29 pKa = 3.67EE30 pKa = 3.76KK31 pKa = 9.97QKK33 pKa = 10.93RR34 pKa = 11.84VDD36 pKa = 3.36EE37 pKa = 4.58CNVIIKK43 pKa = 10.19LIATHH48 pKa = 5.78GRR50 pKa = 11.84KK51 pKa = 9.0FFNYY55 pKa = 7.19EE56 pKa = 3.17ARR58 pKa = 11.84GRR60 pKa = 11.84RR61 pKa = 11.84ISNLLLGPQDD71 pKa = 2.39RR72 pKa = 11.84VYY74 pKa = 11.1FHH76 pKa = 7.65DD77 pKa = 5.75GYY79 pKa = 9.4TLMLIYY85 pKa = 8.21THH87 pKa = 6.93RR88 pKa = 11.84RR89 pKa = 11.84WCRR92 pKa = 11.84WRR94 pKa = 11.84GFSEE98 pKa = 4.3GGTMKK103 pKa = 10.37RR104 pKa = 11.84WVEE107 pKa = 3.77AFRR110 pKa = 11.84DD111 pKa = 4.27YY112 pKa = 10.72ITFGHH117 pKa = 6.98KK118 pKa = 10.68LNINAICQRR127 pKa = 11.84GMGEE131 pKa = 3.65NSGNIWGYY139 pKa = 10.61SDD141 pKa = 4.35EE142 pKa = 4.3EE143 pKa = 4.22AEE145 pKa = 4.47KK146 pKa = 10.88LINQLSKK153 pKa = 11.13LDD155 pKa = 3.63AFNTEE160 pKa = 4.18KK161 pKa = 10.71QGSNN165 pKa = 3.23

Molecular weight: 19.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

9535

44

752

272.4

30.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.422 ± 0.762

1.101 ± 0.175

6.198 ± 0.292

6.765 ± 0.398

3.744 ± 0.175

7.037 ± 0.313

1.657 ± 0.215

6.167 ± 0.313

6.953 ± 0.515

8.128 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.21

4.583 ± 0.275

3.818 ± 0.3

4.206 ± 0.26

4.95 ± 0.31

6.67 ± 0.388

5.684 ± 0.321

6.649 ± 0.344

1.804 ± 0.178

3.115 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski