Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53336 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9IC68|B9IC68_POPTR Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G076300 PE=4 SV=2
MM1 pKa = 7.81DD2 pKa = 4.19RR3 pKa = 11.84VKK5 pKa = 11.18NSVDD9 pKa = 3.21VSPFLLVEE17 pKa = 4.01AAGDD21 pKa = 4.1SEE23 pKa = 4.56VDD25 pKa = 3.22SDD27 pKa = 4.01PTMSTIDD34 pKa = 4.97LVDD37 pKa = 5.01DD38 pKa = 5.26DD39 pKa = 6.28DD40 pKa = 5.25DD41 pKa = 6.6AEE43 pKa = 4.43SCSCDD48 pKa = 3.46TSDD51 pKa = 4.3HH52 pKa = 6.46SCVSDD57 pKa = 4.11IIKK60 pKa = 9.99GACSEE65 pKa = 4.29VEE67 pKa = 4.11AYY69 pKa = 9.01QVNYY73 pKa = 10.83NVVDD77 pKa = 4.31DD78 pKa = 5.72DD79 pKa = 4.69DD80 pKa = 6.63DD81 pKa = 4.44EE82 pKa = 4.94EE83 pKa = 4.63EE84 pKa = 4.27EE85 pKa = 4.36EE86 pKa = 4.58EE87 pKa = 4.39GVEE90 pKa = 4.54VCQSWVHH97 pKa = 5.62HH98 pKa = 5.03VHH100 pKa = 6.06VGLPVKK106 pKa = 10.37QKK108 pKa = 10.32SCVSVDD114 pKa = 3.51SSNEE118 pKa = 3.73SMNEE122 pKa = 3.75KK123 pKa = 10.48EE124 pKa = 4.17KK125 pKa = 11.35DD126 pKa = 3.38RR127 pKa = 11.84LFWEE131 pKa = 4.71ACLASS136 pKa = 3.65

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N7EZH0|A0A3N7EZH0_POPTR Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_005G126600 PE=4 SV=1
MM1 pKa = 7.49SLIIINLHH9 pKa = 5.8HH10 pKa = 6.8LHH12 pKa = 6.68LNHH15 pKa = 6.89HH16 pKa = 6.29LHH18 pKa = 7.33HH19 pKa = 6.72ITTHH23 pKa = 6.15HH24 pKa = 6.98LLHH27 pKa = 6.29QKK29 pKa = 10.02SPPLHH34 pKa = 7.08LITTHH39 pKa = 5.92PHH41 pKa = 5.95LLQRR45 pKa = 11.84SPHH48 pKa = 6.02HH49 pKa = 6.2LHH51 pKa = 5.79TTTHH55 pKa = 6.85PLLLQRR61 pKa = 11.84SLPHH65 pKa = 6.33LHH67 pKa = 5.85TTTHH71 pKa = 6.87PLLHH75 pKa = 6.42LRR77 pKa = 11.84SLHH80 pKa = 6.1HH81 pKa = 6.53LHH83 pKa = 5.97TTTHH87 pKa = 6.85PLLLQRR93 pKa = 11.84SLPHH97 pKa = 6.33LHH99 pKa = 5.69TTTHH103 pKa = 6.43ILLHH107 pKa = 6.08LRR109 pKa = 11.84SLHH112 pKa = 6.1HH113 pKa = 6.53LHH115 pKa = 5.99TTTHH119 pKa = 5.99PHH121 pKa = 5.44LHH123 pKa = 5.46QRR125 pKa = 11.84SLPLHH130 pKa = 6.21HH131 pKa = 6.85TTTHH135 pKa = 6.62PLLLQRR141 pKa = 11.84SLPHH145 pKa = 6.33LHH147 pKa = 5.85TTTHH151 pKa = 6.85PLLLQRR157 pKa = 11.84SLHH160 pKa = 5.99HH161 pKa = 6.36LHH163 pKa = 5.84TTTHH167 pKa = 6.44ILLHH171 pKa = 6.08LRR173 pKa = 11.84SLHH176 pKa = 6.1HH177 pKa = 6.53LHH179 pKa = 5.99TTTHH183 pKa = 5.99PHH185 pKa = 5.44LHH187 pKa = 5.46QRR189 pKa = 11.84SLPLHH194 pKa = 6.21HH195 pKa = 6.85TTTHH199 pKa = 6.62PLLLQRR205 pKa = 11.84SPHH208 pKa = 6.06HH209 pKa = 6.11LHH211 pKa = 5.68TTTHH215 pKa = 6.86LLLHH219 pKa = 6.38LRR221 pKa = 11.84SLPLHH226 pKa = 6.24HH227 pKa = 6.89TTTHH231 pKa = 6.25HH232 pKa = 7.32LLLQRR237 pKa = 11.84SRR239 pKa = 11.84HH240 pKa = 5.42HH241 pKa = 6.35LHH243 pKa = 5.77TTTHH247 pKa = 6.87PLLHH251 pKa = 6.3LRR253 pKa = 11.84SLLLHH258 pKa = 6.35HH259 pKa = 6.69TTTHH263 pKa = 5.8PHH265 pKa = 5.72HH266 pKa = 7.12LLRR269 pKa = 11.84SLHH272 pKa = 6.24HH273 pKa = 6.5LHH275 pKa = 5.66TTIHH279 pKa = 6.73PLLHH283 pKa = 5.94QRR285 pKa = 11.84SLPHH289 pKa = 6.28LHH291 pKa = 5.85TTTHH295 pKa = 6.47HH296 pKa = 7.13LLLQRR301 pKa = 11.84NLHH304 pKa = 6.06HH305 pKa = 6.9LHH307 pKa = 5.74TTTHH311 pKa = 6.68LLSHH315 pKa = 6.7LRR317 pKa = 11.84SIPLHH322 pKa = 5.87HH323 pKa = 6.8TTTHH327 pKa = 5.44PHH329 pKa = 6.38LLRR332 pKa = 11.84RR333 pKa = 11.84SLHH336 pKa = 5.97HH337 pKa = 6.52LHH339 pKa = 5.94TTTHH343 pKa = 7.32LLPLQRR349 pKa = 11.84SLPLHH354 pKa = 7.09HH355 pKa = 6.57ITIHH359 pKa = 5.28PHH361 pKa = 5.26LLRR364 pKa = 11.84RR365 pKa = 11.84SLHH368 pKa = 5.97HH369 pKa = 6.52LHH371 pKa = 5.96TTTHH375 pKa = 6.29LHH377 pKa = 6.01PLRR380 pKa = 11.84RR381 pKa = 11.84NLPLHH386 pKa = 6.03HH387 pKa = 6.66TTTHH391 pKa = 6.78LLLLQRR397 pKa = 11.84SLPHH401 pKa = 6.33LHH403 pKa = 5.86TTTHH407 pKa = 6.56LPLLHH412 pKa = 6.45HH413 pKa = 6.42HH414 pKa = 6.52HH415 pKa = 6.92HH416 pKa = 6.77LLHH419 pKa = 6.15TCMHH423 pKa = 6.79HH424 pKa = 6.88LHH426 pKa = 6.44HH427 pKa = 6.97QNTTKK432 pKa = 10.24MLL434 pKa = 3.99

Molecular weight: 51.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42532

10804

53336

21108701

27

5451

395.8

44.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.522 ± 0.01

1.914 ± 0.006

5.26 ± 0.008

6.492 ± 0.013

4.225 ± 0.006

6.556 ± 0.01

2.395 ± 0.004

5.325 ± 0.008

6.153 ± 0.009

9.837 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.004

4.578 ± 0.007

4.876 ± 0.009

3.8 ± 0.009

5.205 ± 0.009

9.173 ± 0.012

4.864 ± 0.006

6.366 ± 0.008

1.267 ± 0.004

2.739 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski