Burkholderia phage KL3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Tigrvirus; Burkholderia virus KL3

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5E3W6|E5E3W6_9CAUD Gp37 OS=Burkholderia phage KL3 OX=910474 GN=37 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.43VATLQGEE9 pKa = 4.48TLDD12 pKa = 3.94ALCWRR17 pKa = 11.84HH18 pKa = 5.2YY19 pKa = 11.04ASTAGTVEE27 pKa = 4.35AVLEE31 pKa = 4.34ANPGLAEE38 pKa = 4.5LGVVLPMGTVVDD50 pKa = 4.18MPEE53 pKa = 4.49LNTIEE58 pKa = 4.05QTKK61 pKa = 10.13PLLQLFDD68 pKa = 3.71

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5E3X6|E5E3X6_9CAUD Cytosine-specific methyltransferase OS=Burkholderia phage KL3 OX=910474 GN=47 PE=3 SV=1
MM1 pKa = 7.39VDD3 pKa = 3.36VVDD6 pKa = 3.69SATRR10 pKa = 11.84SRR12 pKa = 11.84MMSGIRR18 pKa = 11.84GRR20 pKa = 11.84NTKK23 pKa = 10.25PEE25 pKa = 3.65ILIRR29 pKa = 11.84SLLHH33 pKa = 5.31RR34 pKa = 11.84QGFRR38 pKa = 11.84FRR40 pKa = 11.84LHH42 pKa = 6.58VRR44 pKa = 11.84DD45 pKa = 4.66LPGKK49 pKa = 9.15PDD51 pKa = 3.2IVLPRR56 pKa = 11.84YY57 pKa = 8.47HH58 pKa = 7.12AVIFVHH64 pKa = 6.46GCFWHH69 pKa = 6.81GHH71 pKa = 5.48DD72 pKa = 4.77CSLFKK77 pKa = 11.21LPGTRR82 pKa = 11.84TDD84 pKa = 3.74FWRR87 pKa = 11.84EE88 pKa = 3.53KK89 pKa = 9.99IDD91 pKa = 3.44RR92 pKa = 11.84NRR94 pKa = 11.84ANDD97 pKa = 3.79HH98 pKa = 6.31KK99 pKa = 11.16ALAALRR105 pKa = 11.84TQGWRR110 pKa = 11.84VGIVWEE116 pKa = 4.29CALRR120 pKa = 11.84GANKK124 pKa = 10.17NIEE127 pKa = 4.28SVATGLGQWLYY138 pKa = 11.35SDD140 pKa = 4.79ASTTEE145 pKa = 3.62LRR147 pKa = 11.84GG148 pKa = 3.38

Molecular weight: 16.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12708

45

1075

244.4

26.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.37 ± 0.57

0.779 ± 0.122

6.602 ± 0.286

5.146 ± 0.265

3.557 ± 0.212

8.184 ± 0.497

2.038 ± 0.176

4.422 ± 0.199

4.108 ± 0.242

8.908 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.211

3.187 ± 0.218

4.745 ± 0.321

3.455 ± 0.165

7.782 ± 0.372

5.209 ± 0.238

6.374 ± 0.268

6.83 ± 0.216

1.487 ± 0.122

2.51 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski