Kwoniella bestiolae CBS 10118

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Kwoniella; Kwoniella bestiolae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B9FUC2|A0A1B9FUC2_9TREE Rab-GAP TBC domain-containing protein OS=Kwoniella bestiolae CBS 10118 OX=1296100 GN=I302_08012 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.54SSTTTLTTYY11 pKa = 11.49ALLVLSTIITSVDD24 pKa = 2.68AAGPHH29 pKa = 5.4NRR31 pKa = 11.84FGVGAHH37 pKa = 6.31RR38 pKa = 11.84RR39 pKa = 11.84HH40 pKa = 6.3HH41 pKa = 6.34ARR43 pKa = 11.84AAATSTEE50 pKa = 4.02LSTPVQSSEE59 pKa = 4.03QKK61 pKa = 10.2RR62 pKa = 11.84DD63 pKa = 3.43EE64 pKa = 4.76CEE66 pKa = 3.58LGAWKK71 pKa = 10.53CVGSVLQRR79 pKa = 11.84CYY81 pKa = 10.44NDD83 pKa = 2.64QWNFVTNCTGTDD95 pKa = 4.03IICSDD100 pKa = 3.33GLYY103 pKa = 7.57TTGCVWTWSVEE114 pKa = 4.19QNDD117 pKa = 4.55DD118 pKa = 3.82ANEE121 pKa = 4.24SATSSPPFTSSITVTPTASEE141 pKa = 4.41SSASASTASITNANLAVEE159 pKa = 4.61PTATATADD167 pKa = 3.39ANEE170 pKa = 4.56DD171 pKa = 3.95EE172 pKa = 4.54EE173 pKa = 6.35CEE175 pKa = 5.39DD176 pKa = 4.65EE177 pKa = 6.43DD178 pKa = 4.9EE179 pKa = 5.92QDD181 pKa = 3.97DD182 pKa = 4.61QEE184 pKa = 5.66DD185 pKa = 4.23DD186 pKa = 3.57EE187 pKa = 6.39DD188 pKa = 4.51EE189 pKa = 4.97GEE191 pKa = 4.47EE192 pKa = 4.25YY193 pKa = 10.79CDD195 pKa = 6.01DD196 pKa = 4.4EE197 pKa = 7.72DD198 pKa = 5.92DD199 pKa = 4.71GAWDD203 pKa = 4.25EE204 pKa = 4.79YY205 pKa = 11.08YY206 pKa = 11.12SSATAVVTATPTSAASVVTAAPTSAIGGDD235 pKa = 4.11LAPSDD240 pKa = 5.29DD241 pKa = 4.98DD242 pKa = 6.52DD243 pKa = 5.81EE244 pKa = 7.73GDD246 pKa = 4.58DD247 pKa = 4.15DD248 pKa = 6.34DD249 pKa = 6.15EE250 pKa = 5.11EE251 pKa = 4.76EE252 pKa = 4.24EE253 pKa = 5.54CEE255 pKa = 5.81DD256 pKa = 5.5DD257 pKa = 6.6DD258 pKa = 6.85SDD260 pKa = 6.06DD261 pKa = 4.63YY262 pKa = 12.21DD263 pKa = 4.17DD264 pKa = 5.8ASPTTTSSASTSATSIIGGQLWAGHH289 pKa = 6.4GDD291 pKa = 3.29ASSASTHH298 pKa = 6.44DD299 pKa = 3.35WYY301 pKa = 11.7SWGTQATATTEE312 pKa = 4.15DD313 pKa = 3.81WNTWDD318 pKa = 3.44QATSTSEE325 pKa = 4.42DD326 pKa = 3.37WNTWAQATASASASATSSRR345 pKa = 11.84GHH347 pKa = 6.38GKK349 pKa = 10.69GKK351 pKa = 8.73GHH353 pKa = 7.07KK354 pKa = 9.9SSSADD359 pKa = 3.04WAASTSPSWDD369 pKa = 3.04QSGDD373 pKa = 3.44VSSTTDD379 pKa = 2.83EE380 pKa = 4.22WSTATTTNSWDD391 pKa = 3.56DD392 pKa = 3.28WSGQATASSAAWGQSGKK409 pKa = 11.0GSATASASSSAYY421 pKa = 9.31SAKK424 pKa = 9.9PSKK427 pKa = 10.66GSNHH431 pKa = 4.97TTGSPSSSYY440 pKa = 11.14SSAPHH445 pKa = 4.59YY446 pKa = 10.53VIYY449 pKa = 10.55SDD451 pKa = 3.38YY452 pKa = 10.6WLFEE456 pKa = 4.13MPSVDD461 pKa = 3.51VLSNFNRR468 pKa = 11.84FILAFWMTDD477 pKa = 2.66RR478 pKa = 11.84GAVDD482 pKa = 4.07NAQFWEE488 pKa = 4.21QLDD491 pKa = 3.81STTRR495 pKa = 11.84QQVITDD501 pKa = 3.3YY502 pKa = 10.81HH503 pKa = 5.65NAGIALMVSAFGSTDD518 pKa = 3.12APTSNGADD526 pKa = 3.51PTQTAQALAKK536 pKa = 8.78WVKK539 pKa = 10.57DD540 pKa = 3.6YY541 pKa = 11.68GLDD544 pKa = 3.9GVDD547 pKa = 4.03IDD549 pKa = 4.51YY550 pKa = 11.39EE551 pKa = 4.42DD552 pKa = 3.79MPAMNSAQAVAWIVTFQKK570 pKa = 10.34EE571 pKa = 4.22LRR573 pKa = 11.84RR574 pKa = 11.84LLPSPYY580 pKa = 9.07IISHH584 pKa = 6.48APVAPWFTSANDD596 pKa = 3.86YY597 pKa = 11.02SDD599 pKa = 3.34KK600 pKa = 11.11AYY602 pKa = 10.99VAIHH606 pKa = 6.01EE607 pKa = 4.3QTGDD611 pKa = 3.67GIDD614 pKa = 3.91FYY616 pKa = 11.62NIQFYY621 pKa = 10.48NQGSGVYY628 pKa = 8.56EE629 pKa = 4.02DD630 pKa = 5.17CYY632 pKa = 11.77SLLFDD637 pKa = 5.61SGNDD641 pKa = 3.41WPSTSVFEE649 pKa = 4.69INSSAGLPLDD659 pKa = 4.54KK660 pKa = 10.61IVIGKK665 pKa = 9.09PLDD668 pKa = 3.58EE669 pKa = 4.45GAAANGFIDD678 pKa = 4.33ASLLAKK684 pKa = 10.21CVSQAQAKK692 pKa = 8.22GWNAGVMFWEE702 pKa = 4.49WTTVSPAVPSS712 pKa = 3.68

Molecular weight: 75.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B9GEZ9|A0A1B9GEZ9_9TREE Flavin mononucleotide kinase 1 OS=Kwoniella bestiolae CBS 10118 OX=1296100 GN=I302_01056 PE=3 SV=1
MM1 pKa = 7.85PSLNQLCQNCGLPRR15 pKa = 11.84ASPPPPLDD23 pKa = 3.27TMFEE27 pKa = 4.18EE28 pKa = 4.77DD29 pKa = 4.18FEE31 pKa = 5.19RR32 pKa = 11.84CFICQQPCKK41 pKa = 10.49GLYY44 pKa = 9.88CSSEE48 pKa = 3.78CRR50 pKa = 11.84LRR52 pKa = 11.84DD53 pKa = 3.24QGTPSPAVRR62 pKa = 11.84AAHH65 pKa = 6.13GPVKK69 pKa = 9.77ITSQLPAALSPLVRR83 pKa = 11.84PTQHH87 pKa = 7.16PGRR90 pKa = 11.84SPRR93 pKa = 11.84VLAQNRR99 pKa = 11.84GSSSISSGSSSVSSSPLQSPQTNPSEE125 pKa = 4.15VDD127 pKa = 3.24SPKK130 pKa = 10.24RR131 pKa = 11.84DD132 pKa = 3.71NFDD135 pKa = 4.39LPPPAYY141 pKa = 7.38PTKK144 pKa = 10.7QFGAPASVPMKK155 pKa = 10.38IPALASRR162 pKa = 11.84PSPLVAPSQTPGSVGSTVYY181 pKa = 10.23PAGASIDD188 pKa = 3.47TLRR191 pKa = 11.84YY192 pKa = 8.36GRR194 pKa = 11.84KK195 pKa = 8.05PSAVNSVISPNALIPRR211 pKa = 11.84CACGKK216 pKa = 9.03PANHH220 pKa = 7.4KK221 pKa = 10.24NRR223 pKa = 11.84ASSKK227 pKa = 10.73DD228 pKa = 3.42RR229 pKa = 11.84ADD231 pKa = 4.9LIDD234 pKa = 3.86SGFSRR239 pKa = 11.84LSLGPSLVTAQEE251 pKa = 4.14EE252 pKa = 4.13PLPRR256 pKa = 11.84STRR259 pKa = 11.84IVSEE263 pKa = 4.0SAIPPFISGTPGRR276 pKa = 11.84KK277 pKa = 7.14TVPLGIPSSPQVAPSTSLLSRR298 pKa = 11.84SRR300 pKa = 11.84SDD302 pKa = 4.68PIPGSPQTQRR312 pKa = 11.84KK313 pKa = 9.21LIPQVPAPVITNVITPSHH331 pKa = 6.68RR332 pKa = 11.84EE333 pKa = 4.02SNGMPLSPIVPPATRR348 pKa = 11.84PGRR351 pKa = 11.84NRR353 pKa = 11.84NLEE356 pKa = 4.07VNMDD360 pKa = 3.43SPRR363 pKa = 11.84RR364 pKa = 11.84GRR366 pKa = 11.84SRR368 pKa = 11.84EE369 pKa = 3.92RR370 pKa = 11.84QEE372 pKa = 3.92HH373 pKa = 6.26HH374 pKa = 6.37VGQMTSNFGGPADD387 pKa = 4.34RR388 pKa = 11.84EE389 pKa = 4.26QAPSRR394 pKa = 11.84SRR396 pKa = 11.84TRR398 pKa = 11.84RR399 pKa = 11.84RR400 pKa = 11.84SDD402 pKa = 2.76SRR404 pKa = 11.84EE405 pKa = 3.7RR406 pKa = 11.84EE407 pKa = 3.9RR408 pKa = 11.84EE409 pKa = 3.58RR410 pKa = 11.84GRR412 pKa = 11.84GRR414 pKa = 11.84DD415 pKa = 3.08SRR417 pKa = 11.84EE418 pKa = 3.54RR419 pKa = 11.84EE420 pKa = 3.77RR421 pKa = 11.84EE422 pKa = 3.76RR423 pKa = 11.84DD424 pKa = 3.64HH425 pKa = 7.29SGHH428 pKa = 6.39TSPIKK433 pKa = 9.4QTQTPQILPSWSRR446 pKa = 11.84RR447 pKa = 11.84ASEE450 pKa = 3.8ATADD454 pKa = 3.7RR455 pKa = 11.84RR456 pKa = 11.84KK457 pKa = 10.53VLGEE461 pKa = 3.59VAPAMRR467 pKa = 11.84RR468 pKa = 11.84TASNGKK474 pKa = 9.78KK475 pKa = 10.2SPTYY479 pKa = 10.4DD480 pKa = 3.4EE481 pKa = 4.76EE482 pKa = 4.07EE483 pKa = 4.13KK484 pKa = 10.53QRR486 pKa = 11.84KK487 pKa = 8.54KK488 pKa = 11.23DD489 pKa = 3.68EE490 pKa = 4.07INRR493 pKa = 11.84ASKK496 pKa = 10.61QLGQVFGVAAGG507 pKa = 3.38

Molecular weight: 54.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9131

0

9131

4442483

49

5016

486.5

53.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.382 ± 0.029

1.003 ± 0.009

5.562 ± 0.019

6.529 ± 0.027

3.335 ± 0.015

7.258 ± 0.023

2.325 ± 0.011

5.119 ± 0.017

5.24 ± 0.024

8.764 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.009

3.781 ± 0.016

6.669 ± 0.031

3.883 ± 0.023

5.791 ± 0.022

9.442 ± 0.033

6.101 ± 0.017

5.732 ± 0.018

1.377 ± 0.009

2.635 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski