Cellulophaga phage phi19:3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZWL2|R9ZWL2_9CAUD Uncharacterized protein OS=Cellulophaga phage phi19:3 OX=1327971 GN=Phi19:3_gp092 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.61NKK4 pKa = 8.56ITIIMILMSISFGYY18 pKa = 9.85SQVLVPEE25 pKa = 4.2NTGYY29 pKa = 11.18GYY31 pKa = 11.06DD32 pKa = 3.4NLTTVEE38 pKa = 4.61LNALTKK44 pKa = 10.63KK45 pKa = 10.5KK46 pKa = 10.94VGDD49 pKa = 3.23IYY51 pKa = 11.59YY52 pKa = 10.48NADD55 pKa = 3.03LGYY58 pKa = 9.03HH59 pKa = 5.2VKK61 pKa = 9.49WNGVIFEE68 pKa = 4.6SLASGISGIDD78 pKa = 3.43PADD81 pKa = 3.37QDD83 pKa = 3.85KK84 pKa = 11.33LNNISITQPVNLDD97 pKa = 3.69TVEE100 pKa = 4.31SLANSALQTEE110 pKa = 4.96TDD112 pKa = 3.69PTTTPANIKK121 pKa = 10.45AKK123 pKa = 10.55LEE125 pKa = 4.19SLTGTNRR132 pKa = 11.84LDD134 pKa = 3.27AFAIKK139 pKa = 10.27NLSAQEE145 pKa = 3.96TDD147 pKa = 3.46PNLTKK152 pKa = 11.0SNVEE156 pKa = 3.97GLGISYY162 pKa = 9.46TSLSDD167 pKa = 3.3IPAVSSPLTFGPGFTRR183 pKa = 11.84TGDD186 pKa = 3.71NIVLDD191 pKa = 4.09NPFTTANEE199 pKa = 4.21TTLNSALQSEE209 pKa = 4.65TDD211 pKa = 3.7PLYY214 pKa = 10.96SAWDD218 pKa = 3.41KK219 pKa = 11.41DD220 pKa = 4.07YY221 pKa = 11.58NDD223 pKa = 5.36LINRR227 pKa = 11.84PVNLVTYY234 pKa = 10.27DD235 pKa = 3.75PLTAQDD241 pKa = 3.7VEE243 pKa = 4.39ISVLGGSDD251 pKa = 3.46SEE253 pKa = 4.81SYY255 pKa = 10.7ILEE258 pKa = 4.31FDD260 pKa = 3.96PNGYY264 pKa = 10.14DD265 pKa = 3.5YY266 pKa = 11.39SGEE269 pKa = 4.11SSGTGTVDD277 pKa = 2.9QTIIDD282 pKa = 4.09GSTNAVSGNAVFDD295 pKa = 4.11GLSSKK300 pKa = 11.05LNLSGGTMTGEE311 pKa = 4.38LDD313 pKa = 4.21LSLLMPSSVVSFIGSNTDD331 pKa = 3.35PFSSGSFKK339 pKa = 11.04DD340 pKa = 4.13FNVSDD345 pKa = 4.1GSTLIDD351 pKa = 3.56YY352 pKa = 8.76QFSPTSTALSIRR364 pKa = 11.84TNTLVNQTAYY374 pKa = 7.58TAKK377 pKa = 9.42YY378 pKa = 7.38TLPHH382 pKa = 6.52TGTPVNDD389 pKa = 3.52TDD391 pKa = 6.46LITKK395 pKa = 9.38VYY397 pKa = 11.2ADD399 pKa = 3.58ATYY402 pKa = 10.1GASGSYY408 pKa = 10.67EE409 pKa = 3.92EE410 pKa = 5.03GTFTLTNQDD419 pKa = 2.46TDD421 pKa = 3.69YY422 pKa = 11.9SFISQNNTFVRR433 pKa = 11.84FGDD436 pKa = 3.6LVYY439 pKa = 10.39YY440 pKa = 10.16KK441 pKa = 10.81VYY443 pKa = 9.09ITLNKK448 pKa = 7.14TTNTSDD454 pKa = 3.34VFFTLSSASTLPAHH468 pKa = 6.91ATQSVNNINPYY479 pKa = 10.26FSDD482 pKa = 3.94LLSTSGNLNANATALYY498 pKa = 8.82FQHH501 pKa = 6.67NGNVWYY507 pKa = 10.39LKK509 pKa = 10.28QSYY512 pKa = 9.93AGNIGVNVSNIDD524 pKa = 3.53STSSGNFVISFSGTYY539 pKa = 8.85LTDD542 pKa = 3.05

Molecular weight: 58.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9ZY34|R9ZY34_9CAUD Site specific recombinase tyrosine OS=Cellulophaga phage phi19:3 OX=1327971 GN=Phi19:3_gp036 PE=3 SV=1
MM1 pKa = 7.58EE2 pKa = 4.14IWKK5 pKa = 9.34PIKK8 pKa = 10.3GYY10 pKa = 10.09EE11 pKa = 4.1NIYY14 pKa = 9.89QVSNFGRR21 pKa = 11.84VKK23 pKa = 10.44SLDD26 pKa = 3.19RR27 pKa = 11.84IVFNKK32 pKa = 10.51GNGTRR37 pKa = 11.84CKK39 pKa = 10.16TKK41 pKa = 10.52GRR43 pKa = 11.84VLKK46 pKa = 10.45QSKK49 pKa = 10.59DD50 pKa = 3.22KK51 pKa = 11.39GGYY54 pKa = 10.14LYY56 pKa = 11.1VGLYY60 pKa = 10.7NKK62 pKa = 10.43DD63 pKa = 3.48NEE65 pKa = 4.48KK66 pKa = 9.6TSSIKK71 pKa = 8.59VHH73 pKa = 6.31RR74 pKa = 11.84LVAFSFCSGYY84 pKa = 9.2TEE86 pKa = 4.04GLEE89 pKa = 4.25VNHH92 pKa = 7.29KK93 pKa = 10.87DD94 pKa = 4.39GIRR97 pKa = 11.84DD98 pKa = 3.5NNLYY102 pKa = 10.26TNLEE106 pKa = 4.14WVTRR110 pKa = 11.84SQNIRR115 pKa = 11.84DD116 pKa = 3.62TYY118 pKa = 9.56KK119 pKa = 10.43RR120 pKa = 11.84GRR122 pKa = 11.84ITYY125 pKa = 7.32GQKK128 pKa = 10.74NNASKK133 pKa = 11.07LMDD136 pKa = 4.12RR137 pKa = 11.84DD138 pKa = 3.12IGVISSLYY146 pKa = 10.48DD147 pKa = 3.19SGVSQSIISLAFGVSQSTISNVIKK171 pKa = 10.62NKK173 pKa = 9.74HH174 pKa = 5.46YY175 pKa = 11.08KK176 pKa = 10.11NGLIEE181 pKa = 5.52KK182 pKa = 10.11GLADD186 pKa = 3.63QQRR189 pKa = 11.84TNN191 pKa = 3.23

Molecular weight: 21.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

23542

33

1871

182.5

20.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.944 ± 0.265

1.058 ± 0.137

6.448 ± 0.177

7.616 ± 0.389

4.333 ± 0.194

5.926 ± 0.241

1.355 ± 0.169

7.897 ± 0.249

9.162 ± 0.44

8.168 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.16

6.92 ± 0.252

2.646 ± 0.131

3.25 ± 0.188

3.53 ± 0.18

7.051 ± 0.235

6.525 ± 0.32

5.569 ± 0.156

1.053 ± 0.077

4.282 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski