Streptococcus phage Spn1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2L097|X2L097_9CAUD Uncharacterized protein OS=Streptococcus phage Spn1 OX=1461246 PE=4 SV=1
MM1 pKa = 7.91AILDD5 pKa = 4.57DD6 pKa = 4.75LQALYY11 pKa = 11.25DD12 pKa = 4.06NGWDD16 pKa = 4.07ASFNYY21 pKa = 9.53NGQVCGIFPNSIYY34 pKa = 10.86DD35 pKa = 3.58VVVIIADD42 pKa = 3.53DD43 pKa = 4.13EE44 pKa = 4.47YY45 pKa = 10.96RR46 pKa = 11.84ASSFDD51 pKa = 4.4DD52 pKa = 4.95LISLQIEE59 pKa = 4.74GKK61 pKa = 7.71TLPEE65 pKa = 3.62IMNEE69 pKa = 3.98VEE71 pKa = 4.11VQYY74 pKa = 11.6GG75 pKa = 3.21

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2KPG0|X2KPG0_9CAUD Uncharacterized protein OS=Streptococcus phage Spn1 OX=1461246 PE=4 SV=1
MM1 pKa = 8.02HH2 pKa = 7.61EE3 pKa = 4.27ICPGRR8 pKa = 11.84LTPEE12 pKa = 3.75VTRR15 pKa = 11.84LLNEE19 pKa = 4.12KK20 pKa = 10.51FGTNYY25 pKa = 10.38NKK27 pKa = 10.51SQIGGVRR34 pKa = 11.84KK35 pKa = 9.89RR36 pKa = 11.84LGLAVGKK43 pKa = 10.01VYY45 pKa = 10.4QGRR48 pKa = 11.84LLTKK52 pKa = 9.04EE53 pKa = 3.61QHH55 pKa = 6.88DD56 pKa = 4.56YY57 pKa = 11.46LVLIQKK63 pKa = 10.1NKK65 pKa = 9.26ISRR68 pKa = 11.84NVANEE73 pKa = 3.75MNQKK77 pKa = 10.35FGLSLTEE84 pKa = 4.06KK85 pKa = 10.22QIKK88 pKa = 9.48SYY90 pKa = 10.67RR91 pKa = 11.84RR92 pKa = 11.84NNNLHH97 pKa = 6.24SGLTGRR103 pKa = 11.84FEE105 pKa = 5.1KK106 pKa = 11.07GQTPHH111 pKa = 6.58NKK113 pKa = 8.94GKK115 pKa = 10.51KK116 pKa = 8.0YY117 pKa = 10.9PNMPKK122 pKa = 10.37NGGQFKK128 pKa = 10.52KK129 pKa = 10.86GNRR132 pKa = 11.84PPNYY136 pKa = 9.98VPVGTINYY144 pKa = 6.46TTNGYY149 pKa = 8.85PKK151 pKa = 10.35EE152 pKa = 4.48KK153 pKa = 9.66IGEE156 pKa = 4.09PNQWVLKK163 pKa = 9.51HH164 pKa = 5.61RR165 pKa = 11.84KK166 pKa = 8.01VWEE169 pKa = 4.09EE170 pKa = 3.39HH171 pKa = 6.51HH172 pKa = 6.78GPIPKK177 pKa = 9.74GHH179 pKa = 6.74SIVFLDD185 pKa = 4.36GDD187 pKa = 3.75KK188 pKa = 11.06TNYY191 pKa = 10.28DD192 pKa = 3.57ISNLACLSKK201 pKa = 11.52NEE203 pKa = 4.07IARR206 pKa = 11.84MNQNHH211 pKa = 7.16LFTSNADD218 pKa = 3.59LTKK221 pKa = 10.82SGIGLTKK228 pKa = 9.48LTNKK232 pKa = 9.28IRR234 pKa = 11.84EE235 pKa = 4.16VEE237 pKa = 4.19KK238 pKa = 11.28NGG240 pKa = 3.57

Molecular weight: 27.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12765

43

1550

202.6

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.823 ± 0.547

0.533 ± 0.096

6.173 ± 0.326

7.967 ± 0.529

4.034 ± 0.255

6.385 ± 0.582

1.543 ± 0.191

6.769 ± 0.303

8.703 ± 0.418

8.249 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.212

5.272 ± 0.349

2.742 ± 0.251

3.972 ± 0.175

4.599 ± 0.311

6.15 ± 0.413

6.142 ± 0.309

6.314 ± 0.256

1.355 ± 0.159

3.807 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski