Clostridium phage phiCD24-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8WHQ1|A0A0A8WHQ1_9CAUD Uncharacterized protein OS=Clostridium phage phiCD24-1 OX=1582149 GN=PHICD2401_20026 PE=4 SV=1
MM1 pKa = 7.7ALQYY5 pKa = 10.45CVSEE9 pKa = 4.31NEE11 pKa = 4.34LVSFQYY17 pKa = 11.39GEE19 pKa = 3.85INSRR23 pKa = 11.84DD24 pKa = 3.74FGIVITDD31 pKa = 3.41INEE34 pKa = 3.98LASPEE39 pKa = 3.88RR40 pKa = 11.84RR41 pKa = 11.84FEE43 pKa = 4.42RR44 pKa = 11.84IEE46 pKa = 3.67IPGRR50 pKa = 11.84NGSLILDD57 pKa = 4.18EE58 pKa = 4.86GCYY61 pKa = 10.85SNFNLEE67 pKa = 3.97IEE69 pKa = 4.81CYY71 pKa = 9.99IDD73 pKa = 5.0VDD75 pKa = 3.98GKK77 pKa = 10.72DD78 pKa = 3.43INILASEE85 pKa = 4.37IKK87 pKa = 9.92MLLQTDD93 pKa = 4.31FSYY96 pKa = 11.6KK97 pKa = 10.56NLIISTDD104 pKa = 3.26PNYY107 pKa = 10.5YY108 pKa = 10.1RR109 pKa = 11.84EE110 pKa = 4.19AVCINKK116 pKa = 9.63LDD118 pKa = 3.79LEE120 pKa = 4.66EE121 pKa = 5.73IIKK124 pKa = 10.72DD125 pKa = 3.37LGYY128 pKa = 10.91FKK130 pKa = 9.97ITFEE134 pKa = 4.36CKK136 pKa = 9.22PLKK139 pKa = 10.46KK140 pKa = 10.34EE141 pKa = 4.07LVNNLITINEE151 pKa = 4.19SDD153 pKa = 3.78SKK155 pKa = 11.26LINTGMASYY164 pKa = 10.54PLIKK168 pKa = 10.65VFGNGDD174 pKa = 2.88ITLEE178 pKa = 3.98INNEE182 pKa = 4.15VIEE185 pKa = 4.07LTEE188 pKa = 4.2VQGHH192 pKa = 6.47IYY194 pKa = 9.85IDD196 pKa = 3.89CEE198 pKa = 3.94LMNAYY203 pKa = 9.64KK204 pKa = 9.94IDD206 pKa = 3.01IHH208 pKa = 6.41TNDD211 pKa = 3.26VVNEE215 pKa = 3.89NSRR218 pKa = 11.84MFGDD222 pKa = 4.7FPTFKK227 pKa = 10.2HH228 pKa = 6.11GEE230 pKa = 4.03NIITYY235 pKa = 9.81SGDD238 pKa = 3.32VEE240 pKa = 4.5KK241 pKa = 10.93IEE243 pKa = 4.32IEE245 pKa = 4.12PRR247 pKa = 11.84WVVLL251 pKa = 3.73

Molecular weight: 28.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8WEK1|A0A0A8WEK1_9CAUD Putative phage-related protein OS=Clostridium phage phiCD24-1 OX=1582149 GN=PHICD2401_20036 PE=4 SV=1
MM1 pKa = 7.39GRR3 pKa = 11.84LEE5 pKa = 3.79RR6 pKa = 11.84SKK8 pKa = 11.3NKK10 pKa = 10.19RR11 pKa = 11.84EE12 pKa = 3.88NKK14 pKa = 9.22FNKK17 pKa = 9.0IKK19 pKa = 10.92NVFSFTLVLINLVLAILRR37 pKa = 11.84LIKK40 pKa = 10.35EE41 pKa = 4.16LL42 pKa = 3.81

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13367

36

1842

212.2

24.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.893 ± 0.635

0.883 ± 0.111

5.873 ± 0.199

8.947 ± 0.661

3.613 ± 0.217

5.177 ± 0.266

1.032 ± 0.146

8.985 ± 0.371

10.279 ± 0.563

8.379 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.194

7.548 ± 0.441

1.96 ± 0.227

3.284 ± 0.215

3.441 ± 0.303

6.957 ± 0.43

6.187 ± 0.409

5.08 ± 0.218

0.98 ± 0.126

4.107 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski