Lactobacillus phage Dionysus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Harbinvirus; unclassified Harbinvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8HXM8|A0A3Q8HXM8_9CAUD Uncharacterized protein OS=Lactobacillus phage Dionysus OX=2315484 PE=4 SV=1
MM1 pKa = 7.34TKK3 pKa = 10.36SFNLDD8 pKa = 3.55KK9 pKa = 10.71IFKK12 pKa = 10.55SMNDD16 pKa = 3.01DD17 pKa = 3.32WFLMDD22 pKa = 3.11SHH24 pKa = 8.39AEE26 pKa = 3.99EE27 pKa = 4.74FVTLILYY34 pKa = 9.99DD35 pKa = 3.46IEE37 pKa = 5.34NYY39 pKa = 10.02MIVGRR44 pKa = 11.84TNDD47 pKa = 3.13IVAIDD52 pKa = 4.11YY53 pKa = 11.17ADD55 pKa = 3.39NDD57 pKa = 3.94YY58 pKa = 11.71VEE60 pKa = 6.05DD61 pKa = 3.99YY62 pKa = 11.07QNKK65 pKa = 9.45FDD67 pKa = 6.0DD68 pKa = 4.85LYY70 pKa = 11.31EE71 pKa = 4.28EE72 pKa = 4.35QTGHH76 pKa = 6.45EE77 pKa = 3.98NSQYY81 pKa = 10.17EE82 pKa = 4.54FKK84 pKa = 10.6LVPVGITSVALKK96 pKa = 10.67YY97 pKa = 10.76DD98 pKa = 3.87LL99 pKa = 5.03

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7UPT1|A0A3S7UPT1_9CAUD Uncharacterized protein OS=Lactobacillus phage Dionysus OX=2315484 PE=4 SV=1
MM1 pKa = 7.5LTRR4 pKa = 11.84KK5 pKa = 10.05EE6 pKa = 4.09EE7 pKa = 4.26IKK9 pKa = 10.35MKK11 pKa = 10.36LYY13 pKa = 10.6KK14 pKa = 10.63LLIAVVAGVTLSLVTAYY31 pKa = 10.63NADD34 pKa = 3.41ASRR37 pKa = 11.84PNYY40 pKa = 10.55AYY42 pKa = 9.54TLTRR46 pKa = 11.84TKK48 pKa = 10.29QHH50 pKa = 5.87AVKK53 pKa = 9.12LTNTGRR59 pKa = 11.84TEE61 pKa = 3.42NMYY64 pKa = 10.48RR65 pKa = 11.84VTVKK69 pKa = 9.57TGKK72 pKa = 10.0AVRR75 pKa = 11.84WYY77 pKa = 11.31YY78 pKa = 9.8MALNAKK84 pKa = 9.18QSWLVKK90 pKa = 9.53QPRR93 pKa = 11.84KK94 pKa = 9.96YY95 pKa = 9.44SVTVRR100 pKa = 11.84RR101 pKa = 11.84VSKK104 pKa = 10.81SDD106 pKa = 3.2EE107 pKa = 4.01KK108 pKa = 11.29RR109 pKa = 11.84NADD112 pKa = 3.57PRR114 pKa = 11.84NHH116 pKa = 5.76FTPLGIRR123 pKa = 11.84NTQRR127 pKa = 11.84TVWNRR132 pKa = 2.9

Molecular weight: 15.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

172

0

172

40880

43

1421

237.7

26.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.15 ± 0.209

0.538 ± 0.057

7.339 ± 0.195

5.717 ± 0.225

3.464 ± 0.113

6.235 ± 0.297

1.566 ± 0.099

6.48 ± 0.145

7.962 ± 0.301

8.452 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.081

6.818 ± 0.147

2.889 ± 0.138

3.545 ± 0.138

3.493 ± 0.133

7.718 ± 0.32

6.629 ± 0.315

6.676 ± 0.136

1.037 ± 0.067

4.936 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski