Burkholderiales bacterium 8X

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4766 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653JT96|A0A653JT96_9BURK LysR family transcriptional regulator OS=Burkholderiales bacterium 8X OX=2653183 GN=VARIO8X_50455 PE=3 SV=1
MM1 pKa = 6.58TTYY4 pKa = 10.9PMPLEE9 pKa = 3.95QAVIDD14 pKa = 3.96AVLDD18 pKa = 3.73LFEE21 pKa = 6.42ASGSPAFCLTIPGTDD36 pKa = 3.23PTIYY40 pKa = 9.79IACGEE45 pKa = 4.18AGSINIMMPDD55 pKa = 3.42AVNDD59 pKa = 3.88FTVSEE64 pKa = 4.22AASNQAFDD72 pKa = 4.0DD73 pKa = 4.13HH74 pKa = 7.56SNLPVQDD81 pKa = 4.76PKK83 pKa = 11.65DD84 pKa = 4.06DD85 pKa = 4.27DD86 pKa = 4.2PRR88 pKa = 11.84ASDD91 pKa = 3.32SPGRR95 pKa = 11.84ARR97 pKa = 11.84GCSDD101 pKa = 2.9

Molecular weight: 10.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653JP78|A0A653JP78_9BURK Beta-alanine--pyruvate aminotransferase OS=Burkholderiales bacterium 8X OX=2653183 GN=bauA PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84SLRR5 pKa = 11.84PPTRR9 pKa = 11.84RR10 pKa = 11.84PLAIRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84GPGAKK22 pKa = 9.1SHH24 pKa = 4.25VRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84GSLLRR33 pKa = 11.84GKK35 pKa = 10.34ARR37 pKa = 11.84GLAGLSAARR46 pKa = 11.84SPQSTLHH53 pKa = 6.65RR54 pKa = 11.84FTVPPFHH61 pKa = 7.33RR62 pKa = 11.84FTVSAIGRR70 pKa = 11.84LARR73 pKa = 11.84PRR75 pKa = 11.84LTTPPP80 pKa = 3.6

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4766

0

4766

1542289

20

2883

323.6

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.997 ± 0.046

0.952 ± 0.011

5.282 ± 0.023

5.344 ± 0.031

3.516 ± 0.024

8.716 ± 0.043

2.22 ± 0.023

4.297 ± 0.027

3.093 ± 0.038

10.368 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.017

2.478 ± 0.024

5.544 ± 0.027

3.678 ± 0.023

7.695 ± 0.053

5.749 ± 0.029

4.871 ± 0.031

7.397 ± 0.03

1.402 ± 0.014

2.027 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski