Amino acid dipepetide frequency for Novosphingobium sp. Rr 2-17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.68AlaAla: 17.68 ± 0.19	1.051AlaCys: 1.051 ± 0.035	7.258AlaAsp: 7.258 ± 0.089	6.683AlaGlu: 6.683 ± 0.098	4.248AlaPhe: 4.248 ± 0.058	10.914AlaGly: 10.914 ± 0.097	2.436AlaHis: 2.436 ± 0.05	6.808AlaIle: 6.808 ± 0.074	4.209AlaLys: 4.209 ± 0.074	13.924AlaLeu: 13.924 ± 0.131	3.851AlaMet: 3.851 ± 0.059	3.262AlaAsn: 3.262 ± 0.055	6.167AlaPro: 6.167 ± 0.08	5.055AlaGln: 5.055 ± 0.076	9.2AlaArg: 9.2 ± 0.109	7.172AlaSer: 7.172 ± 0.08	6.695AlaThr: 6.695 ± 0.084	8.462AlaVal: 8.462 ± 0.088	1.712AlaTrp: 1.712 ± 0.04	2.673AlaTyr: 2.673 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.0CysAla: 1.0 ± 0.026	0.106CysCys: 0.106 ± 0.008	0.533CysAsp: 0.533 ± 0.019	0.498CysGlu: 0.498 ± 0.018	0.261CysPhe: 0.261 ± 0.013	0.866CysGly: 0.866 ± 0.029	0.218CysHis: 0.218 ± 0.015	0.368CysIle: 0.368 ± 0.02	0.189CysLys: 0.189 ± 0.012	0.773CysLeu: 0.773 ± 0.028	0.158CysMet: 0.158 ± 0.012	0.212CysAsn: 0.212 ± 0.014	0.431CysPro: 0.431 ± 0.02	0.217CysGln: 0.217 ± 0.013	0.577CysArg: 0.577 ± 0.024	0.491CysSer: 0.491 ± 0.018	0.387CysThr: 0.387 ± 0.018	0.603CysVal: 0.603 ± 0.021	0.125CysTrp: 0.125 ± 0.01	0.171CysTyr: 0.171 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.404AspAla: 7.404 ± 0.084	0.488AspCys: 0.488 ± 0.021	3.171AspAsp: 3.171 ± 0.051	3.185AspGlu: 3.185 ± 0.06	2.095AspPhe: 2.095 ± 0.04	5.411AspGly: 5.411 ± 0.071	1.292AspHis: 1.292 ± 0.032	2.752AspIle: 2.752 ± 0.052	1.897AspLys: 1.897 ± 0.039	5.838AspLeu: 5.838 ± 0.065	1.325AspMet: 1.325 ± 0.038	1.47AspAsn: 1.47 ± 0.036	3.509AspPro: 3.509 ± 0.057	1.866AspGln: 1.866 ± 0.04	4.439AspArg: 4.439 ± 0.067	2.293AspSer: 2.293 ± 0.046	2.816AspThr: 2.816 ± 0.049	4.168AspVal: 4.168 ± 0.055	1.082AspTrp: 1.082 ± 0.029	1.626AspTyr: 1.626 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	7.296GluAla: 7.296 ± 0.093	0.332GluCys: 0.332 ± 0.015	2.7GluAsp: 2.7 ± 0.053	2.647GluGlu: 2.647 ± 0.058	1.482GluPhe: 1.482 ± 0.032	4.267GluGly: 4.267 ± 0.057	1.215GluHis: 1.215 ± 0.031	2.884GluIle: 2.884 ± 0.051	1.851GluLys: 1.851 ± 0.046	4.95GluLeu: 4.95 ± 0.062	1.323GluMet: 1.323 ± 0.035	1.367GluAsn: 1.367 ± 0.03	2.515GluPro: 2.515 ± 0.045	2.052GluGln: 2.052 ± 0.041	4.581GluArg: 4.581 ± 0.067	2.115GluSer: 2.115 ± 0.039	3.153GluThr: 3.153 ± 0.049	3.59GluVal: 3.59 ± 0.051	0.791GluTrp: 0.791 ± 0.023	1.056GluTyr: 1.056 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.767PheAla: 4.767 ± 0.066	0.341PheCys: 0.341 ± 0.014	2.562PheAsp: 2.562 ± 0.041	1.98PheGlu: 1.98 ± 0.04	1.208PhePhe: 1.208 ± 0.035	3.497PheGly: 3.497 ± 0.054	0.704PheHis: 0.704 ± 0.024	1.315PheIle: 1.315 ± 0.031	0.974PheLys: 0.974 ± 0.026	3.009PheLeu: 3.009 ± 0.049	0.729PheMet: 0.729 ± 0.025	1.046PheAsn: 1.046 ± 0.029	1.413PhePro: 1.413 ± 0.034	0.954PheGln: 0.954 ± 0.027	2.119PheArg: 2.119 ± 0.038	2.126PheSer: 2.126 ± 0.042	2.102PheThr: 2.102 ± 0.038	2.693PheVal: 2.693 ± 0.049	0.5PheTrp: 0.5 ± 0.025	0.917PheTyr: 0.917 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.713GlyAla: 9.713 ± 0.099	0.828GlyCys: 0.828 ± 0.023	4.635GlyAsp: 4.635 ± 0.07	4.53GlyGlu: 4.53 ± 0.057	3.449GlyPhe: 3.449 ± 0.058	7.716GlyGly: 7.716 ± 0.109	1.878GlyHis: 1.878 ± 0.037	4.398GlyIle: 4.398 ± 0.053	3.425GlyLys: 3.425 ± 0.05	8.795GlyLeu: 8.795 ± 0.101	2.333GlyMet: 2.333 ± 0.045	2.32GlyAsn: 2.32 ± 0.051	3.638GlyPro: 3.638 ± 0.057	3.084GlyGln: 3.084 ± 0.049	6.031GlyArg: 6.031 ± 0.07	5.156GlySer: 5.156 ± 0.08	5.092GlyThr: 5.092 ± 0.099	6.229GlyVal: 6.229 ± 0.068	1.553GlyTrp: 1.553 ± 0.036	2.464GlyTyr: 2.464 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.435HisAla: 2.435 ± 0.051	0.239HisCys: 0.239 ± 0.013	1.264HisAsp: 1.264 ± 0.035	1.043HisGlu: 1.043 ± 0.028	0.833HisPhe: 0.833 ± 0.028	1.917HisGly: 1.917 ± 0.043	0.586HisHis: 0.586 ± 0.025	0.915HisIle: 0.915 ± 0.027	0.478HisLys: 0.478 ± 0.017	1.935HisLeu: 1.935 ± 0.038	0.458HisMet: 0.458 ± 0.022	0.505HisAsn: 0.505 ± 0.02	1.256HisPro: 1.256 ± 0.029	0.64HisGln: 0.64 ± 0.018	1.468HisArg: 1.468 ± 0.035	1.106HisSer: 1.106 ± 0.028	0.873HisThr: 0.873 ± 0.026	1.51HisVal: 1.51 ± 0.035	0.38HisTrp: 0.38 ± 0.017	0.581HisTyr: 0.581 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.695IleAla: 7.695 ± 0.084	0.454IleCys: 0.454 ± 0.019	3.857IleAsp: 3.857 ± 0.059	3.58IleGlu: 3.58 ± 0.061	1.411IlePhe: 1.411 ± 0.035	4.947IleGly: 4.947 ± 0.07	0.902IleHis: 0.902 ± 0.027	1.872IleIle: 1.872 ± 0.035	1.293IleLys: 1.293 ± 0.033	3.755IleLeu: 3.755 ± 0.057	0.844IleMet: 0.844 ± 0.023	1.313IleAsn: 1.313 ± 0.032	2.206IlePro: 2.206 ± 0.042	1.234IleGln: 1.234 ± 0.027	3.022IleArg: 3.022 ± 0.046	2.783IleSer: 2.783 ± 0.047	2.643IleThr: 2.643 ± 0.05	4.11IleVal: 4.11 ± 0.058	0.579IleTrp: 0.579 ± 0.022	1.098IleTyr: 1.098 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.125LysAla: 4.125 ± 0.063	0.155LysCys: 0.155 ± 0.01	1.632LysAsp: 1.632 ± 0.038	1.259LysGlu: 1.259 ± 0.037	0.867LysPhe: 0.867 ± 0.024	2.713LysGly: 2.713 ± 0.048	0.543LysHis: 0.543 ± 0.021	1.563LysIle: 1.563 ± 0.037	1.081LysLys: 1.081 ± 0.036	3.326LysLeu: 3.326 ± 0.056	0.746LysMet: 0.746 ± 0.024	0.833LysAsn: 0.833 ± 0.03	2.068LysPro: 2.068 ± 0.045	1.043LysGln: 1.043 ± 0.028	2.266LysArg: 2.266 ± 0.044	1.68LysSer: 1.68 ± 0.037	1.821LysThr: 1.821 ± 0.039	2.414LysVal: 2.414 ± 0.041	0.398LysTrp: 0.398 ± 0.018	0.635LysTyr: 0.635 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.127LeuAla: 14.127 ± 0.126	0.863LeuCys: 0.863 ± 0.025	6.131LeuAsp: 6.131 ± 0.069	4.791LeuGlu: 4.791 ± 0.055	3.466LeuPhe: 3.466 ± 0.057	8.418LeuGly: 8.418 ± 0.097	1.875LeuHis: 1.875 ± 0.032	4.611LeuIle: 4.611 ± 0.061	3.055LeuLys: 3.055 ± 0.051	9.5LeuLeu: 9.5 ± 0.113	2.137LeuMet: 2.137 ± 0.041	2.454LeuAsn: 2.454 ± 0.043	5.757LeuPro: 5.757 ± 0.077	2.625LeuGln: 2.625 ± 0.047	6.89LeuArg: 6.89 ± 0.08	6.428LeuSer: 6.428 ± 0.07	5.743LeuThr: 5.743 ± 0.074	7.401LeuVal: 7.401 ± 0.077	1.175LeuTrp: 1.175 ± 0.03	2.022LeuTyr: 2.022 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	3.082MetAla: 3.082 ± 0.054	0.153MetCys: 0.153 ± 0.01	1.105MetAsp: 1.105 ± 0.03	1.017MetGlu: 1.017 ± 0.031	0.645MetPhe: 0.645 ± 0.024	1.82MetGly: 1.82 ± 0.041	0.428MetHis: 0.428 ± 0.017	1.308MetIle: 1.308 ± 0.036	0.896MetLys: 0.896 ± 0.024	2.548MetLeu: 2.548 ± 0.044	0.595MetMet: 0.595 ± 0.021	0.687MetAsn: 0.687 ± 0.022	1.564MetPro: 1.564 ± 0.035	0.693MetGln: 0.693 ± 0.025	1.74MetArg: 1.74 ± 0.036	1.494MetSer: 1.494 ± 0.034	1.764MetThr: 1.764 ± 0.038	1.698MetVal: 1.698 ± 0.035	0.2MetTrp: 0.2 ± 0.012	0.269MetTyr: 0.269 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.377AsnAla: 3.377 ± 0.055	0.244AsnCys: 0.244 ± 0.015	1.451AsnAsp: 1.451 ± 0.032	1.153AsnGlu: 1.153 ± 0.027	1.019AsnPhe: 1.019 ± 0.027	2.477AsnGly: 2.477 ± 0.05	0.488AsnHis: 0.488 ± 0.018	1.245AsnIle: 1.245 ± 0.036	0.722AsnLys: 0.722 ± 0.026	2.635AsnLeu: 2.635 ± 0.044	0.521AsnMet: 0.521 ± 0.02	0.684AsnAsn: 0.684 ± 0.031	1.795AsnPro: 1.795 ± 0.042	0.792AsnGln: 0.792 ± 0.027	1.86AsnArg: 1.86 ± 0.041	1.374AsnSer: 1.374 ± 0.047	1.254AsnThr: 1.254 ± 0.04	2.082AsnVal: 2.082 ± 0.048	0.427AsnTrp: 0.427 ± 0.018	0.79AsnTyr: 0.79 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	6.837ProAla: 6.837 ± 0.083	0.344ProCys: 0.344 ± 0.017	3.545ProAsp: 3.545 ± 0.056	3.379ProGlu: 3.379 ± 0.052	1.909ProPhe: 1.909 ± 0.039	4.818ProGly: 4.818 ± 0.066	1.052ProHis: 1.052 ± 0.028	2.456ProIle: 2.456 ± 0.045	1.625ProLys: 1.625 ± 0.035	4.812ProLeu: 4.812 ± 0.071	1.174ProMet: 1.174 ± 0.035	1.314ProAsn: 1.314 ± 0.034	2.898ProPro: 2.898 ± 0.076	1.876ProGln: 1.876 ± 0.04	2.983ProArg: 2.983 ± 0.049	3.13ProSer: 3.13 ± 0.047	2.742ProThr: 2.742 ± 0.045	4.22ProVal: 4.22 ± 0.062	0.722ProTrp: 0.722 ± 0.024	1.112ProTyr: 1.112 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.439GlnAla: 4.439 ± 0.059	0.239GlnCys: 0.239 ± 0.013	1.635GlnAsp: 1.635 ± 0.033	1.375GlnGlu: 1.375 ± 0.03	1.161GlnPhe: 1.161 ± 0.031	2.747GlnGly: 2.747 ± 0.046	0.682GlnHis: 0.682 ± 0.025	1.89GlnIle: 1.89 ± 0.038	0.938GlnLys: 0.938 ± 0.027	3.112GlnLeu: 3.112 ± 0.052	0.803GlnMet: 0.803 ± 0.027	0.836GlnAsn: 0.836 ± 0.024	1.916GlnPro: 1.916 ± 0.036	1.2GlnGln: 1.2 ± 0.038	2.518GlnArg: 2.518 ± 0.048	1.909GlnSer: 1.909 ± 0.038	1.87GlnThr: 1.87 ± 0.04	2.686GlnVal: 2.686 ± 0.044	0.498GlnTrp: 0.498 ± 0.02	0.726GlnTyr: 0.726 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	8.133ArgAla: 8.133 ± 0.088	0.49ArgCys: 0.49 ± 0.018	4.046ArgAsp: 4.046 ± 0.058	3.872ArgGlu: 3.872 ± 0.061	2.999ArgPhe: 2.999 ± 0.048	4.873ArgGly: 4.873 ± 0.071	1.7ArgHis: 1.7 ± 0.04	4.028ArgIle: 4.028 ± 0.062	2.259ArgLys: 2.259 ± 0.044	7.782ArgLeu: 7.782 ± 0.084	1.862ArgMet: 1.862 ± 0.036	1.906ArgAsn: 1.906 ± 0.044	3.528ArgPro: 3.528 ± 0.054	2.632ArgGln: 2.632 ± 0.049	5.525ArgArg: 5.525 ± 0.077	3.829ArgSer: 3.829 ± 0.057	3.579ArgThr: 3.579 ± 0.05	4.586ArgVal: 4.586 ± 0.056	1.17ArgTrp: 1.17 ± 0.033	1.984ArgTyr: 1.984 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.989SerAla: 6.989 ± 0.076	0.422SerCys: 0.422 ± 0.023	3.261SerAsp: 3.261 ± 0.055	2.655SerGlu: 2.655 ± 0.048	2.155SerPhe: 2.155 ± 0.044	5.703SerGly: 5.703 ± 0.096	1.112SerHis: 1.112 ± 0.031	2.797SerIle: 2.797 ± 0.055	1.6SerLys: 1.6 ± 0.033	5.596SerLeu: 5.596 ± 0.067	1.239SerMet: 1.239 ± 0.03	1.567SerAsn: 1.567 ± 0.048	3.02SerPro: 3.02 ± 0.043	1.897SerGln: 1.897 ± 0.043	3.671SerArg: 3.671 ± 0.062	3.292SerSer: 3.292 ± 0.067	3.056SerThr: 3.056 ± 0.064	3.932SerVal: 3.932 ± 0.053	0.787SerTrp: 0.787 ± 0.029	1.526SerTyr: 1.526 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.688ThrAla: 6.688 ± 0.08	0.423ThrCys: 0.423 ± 0.018	2.935ThrAsp: 2.935 ± 0.049	2.355ThrGlu: 2.355 ± 0.047	1.927ThrPhe: 1.927 ± 0.039	5.378ThrGly: 5.378 ± 0.082	1.046ThrHis: 1.046 ± 0.029	3.058ThrIle: 3.058 ± 0.064	1.472ThrLys: 1.472 ± 0.034	6.022ThrLeu: 6.022 ± 0.073	1.215ThrMet: 1.215 ± 0.028	1.392ThrAsn: 1.392 ± 0.043	3.539ThrPro: 3.539 ± 0.054	1.759ThrGln: 1.759 ± 0.032	3.457ThrArg: 3.457 ± 0.049	3.235ThrSer: 3.235 ± 0.061	3.013ThrThr: 3.013 ± 0.053	4.33ThrVal: 4.33 ± 0.05	0.705ThrTrp: 0.705 ± 0.022	1.391ThrTyr: 1.391 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	9.471ValAla: 9.471 ± 0.088	0.653ValCys: 0.653 ± 0.022	4.096ValAsp: 4.096 ± 0.051	4.327ValGlu: 4.327 ± 0.061	2.347ValPhe: 2.347 ± 0.046	5.621ValGly: 5.621 ± 0.058	1.387ValHis: 1.387 ± 0.031	3.793ValIle: 3.793 ± 0.066	2.022ValLys: 2.022 ± 0.044	7.205ValLeu: 7.205 ± 0.081	1.653ValMet: 1.653 ± 0.037	1.976ValAsn: 1.976 ± 0.045	3.984ValPro: 3.984 ± 0.063	2.101ValGln: 2.101 ± 0.042	5.17ValArg: 5.17 ± 0.072	4.446ValSer: 4.446 ± 0.06	4.671ValThr: 4.671 ± 0.065	5.644ValVal: 5.644 ± 0.083	0.86ValTrp: 0.86 ± 0.026	1.413ValTyr: 1.413 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.356TrpAla: 1.356 ± 0.032	0.139TrpCys: 0.139 ± 0.01	0.68TrpAsp: 0.68 ± 0.021	0.604TrpGlu: 0.604 ± 0.024	0.567TrpPhe: 0.567 ± 0.021	0.974TrpGly: 0.974 ± 0.03	0.363TrpHis: 0.363 ± 0.016	0.683TrpIle: 0.683 ± 0.022	0.506TrpLys: 0.506 ± 0.023	1.77TrpLeu: 1.77 ± 0.045	0.345TrpMet: 0.345 ± 0.015	0.503TrpAsn: 0.503 ± 0.02	0.698TrpPro: 0.698 ± 0.026	0.66TrpGln: 0.66 ± 0.021	1.326TrpArg: 1.326 ± 0.031	0.893TrpSer: 0.893 ± 0.027	0.838TrpThr: 0.838 ± 0.025	0.831TrpVal: 0.831 ± 0.024	0.248TrpTrp: 0.248 ± 0.015	0.281TrpTyr: 0.281 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.844TyrAla: 2.844 ± 0.043	0.221TyrCys: 0.221 ± 0.014	1.632TyrAsp: 1.632 ± 0.037	1.235TyrGlu: 1.235 ± 0.034	0.905TyrPhe: 0.905 ± 0.026	2.19TyrGly: 2.19 ± 0.047	0.512TyrHis: 0.512 ± 0.021	0.856TyrIle: 0.856 ± 0.026	0.676TyrLys: 0.676 ± 0.022	2.182TyrLeu: 2.182 ± 0.041	0.4TyrMet: 0.4 ± 0.017	0.746TyrAsn: 0.746 ± 0.03	1.044TyrPro: 1.044 ± 0.025	0.799TyrGln: 0.799 ± 0.024	1.939TyrArg: 1.939 ± 0.041	1.326TyrSer: 1.326 ± 0.038	1.226TyrThr: 1.226 ± 0.035	1.651TyrVal: 1.651 ± 0.04	0.353TyrTrp: 0.353 ± 0.017	0.713TyrTyr: 0.713 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4301 proteins (1333011 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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