Cellulophaga phage phi48:2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA bacterial viruses

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZY19|R9ZY19_9VIRU Uncharacterized protein OS=Cellulophaga phage phi48:2 OX=1327968 GN=Phi48:2_gp04 PE=4 SV=1
MM1 pKa = 7.36SNKK4 pKa = 9.96EE5 pKa = 3.95IEE7 pKa = 4.3KK8 pKa = 10.41LDD10 pKa = 3.59YY11 pKa = 10.51TLEE14 pKa = 4.18EE15 pKa = 3.99LCNRR19 pKa = 11.84YY20 pKa = 10.05DD21 pKa = 4.65KK22 pKa = 11.38EE23 pKa = 4.22DD24 pKa = 3.43AKK26 pKa = 10.54LTNFINILNYY36 pKa = 10.0VLAFVLAVAFGYY48 pKa = 9.6FINTLIFF55 pKa = 4.04

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9ZZ84|R9ZZ84_9VIRU Uncharacterized protein OS=Cellulophaga phage phi48:2 OX=1327968 GN=Phi48:2_gp25 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 9.96YY4 pKa = 9.98PKK6 pKa = 10.4VPSDD10 pKa = 5.65KK11 pKa = 10.49KK12 pKa = 11.01NKK14 pKa = 8.76WNLLSSSKK22 pKa = 10.62FEE24 pKa = 4.34KK25 pKa = 10.54SYY27 pKa = 10.12EE28 pKa = 4.13NKK30 pKa = 10.02NRR32 pKa = 11.84FARR35 pKa = 11.84HH36 pKa = 5.67RR37 pKa = 11.84YY38 pKa = 7.84KK39 pKa = 10.7TFDD42 pKa = 3.48YY43 pKa = 10.78EE44 pKa = 4.94KK45 pKa = 10.97GILYY49 pKa = 8.36YY50 pKa = 10.43QNSRR54 pKa = 11.84EE55 pKa = 4.18RR56 pKa = 11.84ITKK59 pKa = 7.55TFKK62 pKa = 10.7LKK64 pKa = 10.45KK65 pKa = 9.87KK66 pKa = 10.25RR67 pKa = 3.59

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

3666

44

662

126.4

14.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.201 ± 0.6

0.982 ± 0.199

4.801 ± 0.357

5.919 ± 0.464

6.71 ± 0.643

5.128 ± 0.531

1.637 ± 0.341

8.347 ± 0.538

10.011 ± 0.848

10.065 ± 0.597

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.219

6.929 ± 0.624

2.237 ± 0.308

2.673 ± 0.302

3.546 ± 0.298

6.847 ± 0.465

6.001 ± 0.573

6.028 ± 0.53

0.709 ± 0.206

4.801 ± 0.47
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski