Escherichia phage Bp4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Escherichia virus Bp4

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2KMH7|X2KMH7_9CAUD Uncharacterized protein OS=Escherichia phage Bp4 OX=1458848 GN=EpBp4_0008 PE=4 SV=1
MM1 pKa = 7.53LGVPITFIKK10 pKa = 10.02IDD12 pKa = 3.5TSIEE16 pKa = 3.9VPEE19 pKa = 4.09NVVVYY24 pKa = 9.81IYY26 pKa = 10.51SSCEE30 pKa = 3.66PMVVTTLTGRR40 pKa = 11.84LITLYY45 pKa = 10.11PSTSYY50 pKa = 11.06LSSDD54 pKa = 3.71EE55 pKa = 4.32SCC57 pKa = 3.97

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7X6Y9|A0A0A7X6Y9_9CAUD Uncharacterized protein OS=Escherichia phage Bp4 OX=1458848 GN=EpBp4_0090 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.01SLAAILVMLFILAVYY17 pKa = 8.21LIPTIIAFARR27 pKa = 11.84GHH29 pKa = 5.64ASKK32 pKa = 10.1WGIGVLNIVLGWSLVFWVVALIWSLSNKK60 pKa = 9.07GRR62 pKa = 11.84TRR64 pKa = 11.84LQMM67 pKa = 4.05

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

21673

34

3570

230.6

25.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.637 ± 0.579

0.895 ± 0.17

5.657 ± 0.183

6.34 ± 0.278

3.613 ± 0.175

6.778 ± 0.267

1.776 ± 0.168

5.772 ± 0.238

6.367 ± 0.267

8.365 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.953 ± 0.119

5.717 ± 0.164

4.093 ± 0.158

4.305 ± 0.261

4.328 ± 0.164

6.178 ± 0.213

6.437 ± 0.278

7.009 ± 0.209

1.19 ± 0.118

3.59 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski