Nitrosomonas sp. Nm51

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2986 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9NHP9|A0A1H9NHP9_9PROT Uncharacterized protein OS=Nitrosomonas sp. Nm51 OX=133720 GN=SAMN05421690_10217 PE=4 SV=1
MM1 pKa = 7.14IRR3 pKa = 11.84KK4 pKa = 8.77VPTFVSHH11 pKa = 7.22LPPPNPTGISVLSTVEE27 pKa = 3.96VNSFQTTGITIDD39 pKa = 4.34SPDD42 pKa = 3.88FPNPLASFMQNLSSCIVDD60 pKa = 4.34APADD64 pKa = 4.39LINLVVNTDD73 pKa = 2.34ICYY76 pKa = 10.72DD77 pKa = 3.55LTGQLSGFPSGFGITINPPVTVSTVSSSINRR108 pKa = 11.84QVADD112 pKa = 4.33CFATDD117 pKa = 3.37AAVINNPVTGEE128 pKa = 3.69VWVNVDD134 pKa = 3.15GSFVPVSEE142 pKa = 4.47SSGFF146 pKa = 3.42

Molecular weight: 15.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9LWS3|A0A1H9LWS3_9PROT Metal-dependent hydrolase endonuclease/exonuclease/phosphatase family OS=Nitrosomonas sp. Nm51 OX=133720 GN=SAMN05421690_101047 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTKK11 pKa = 10.59RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.03TRR25 pKa = 11.84SGAAIIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.88GRR39 pKa = 11.84ARR41 pKa = 11.84LGVV44 pKa = 3.34

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2986

0

2986

914779

24

3193

306.4

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.088 ± 0.05

1.059 ± 0.018

5.475 ± 0.045

5.894 ± 0.052

4.074 ± 0.032

6.919 ± 0.053

2.512 ± 0.025

6.736 ± 0.041

4.748 ± 0.051

10.336 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.024

4.191 ± 0.038

4.296 ± 0.034

4.321 ± 0.036

5.634 ± 0.043

6.021 ± 0.038

5.456 ± 0.049

6.545 ± 0.04

1.274 ± 0.021

2.945 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski