Pseudomonas phage KPP21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Migulavirinae; Luzseptimavirus; Pseudomonas virus KPP21

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H5AXU9|A0A0H5AXU9_BPK21 Uncharacterized protein OS=Pseudomonas phage KPP21 OX=1678082 PE=4 SV=1
MM1 pKa = 7.8AEE3 pKa = 4.52DD4 pKa = 3.87DD5 pKa = 4.15TVEE8 pKa = 4.16EE9 pKa = 4.16AWLLLWSLHH18 pKa = 6.73DD19 pKa = 4.35KK20 pKa = 8.4YY21 pKa = 9.62TKK23 pKa = 10.46EE24 pKa = 4.05GLSNPNLQLGDD35 pKa = 4.19LDD37 pKa = 5.48DD38 pKa = 4.73GPFYY42 pKa = 10.93EE43 pKa = 6.02DD44 pKa = 3.44YY45 pKa = 11.38DD46 pKa = 4.03EE47 pKa = 4.64GTEE50 pKa = 4.16EE51 pKa = 4.33PACSGDD57 pKa = 3.24AA58 pKa = 3.71

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H5BI79|A0A0H5BI79_BPK21 Uncharacterized protein OS=Pseudomonas phage KPP21 OX=1678082 PE=4 SV=1
MM1 pKa = 7.92LILQLVWLFLVFAAVTFWVSRR22 pKa = 11.84MEE24 pKa = 4.36PKK26 pKa = 10.57RR27 pKa = 11.84PWGWLWWVWLGVLAVSSHH45 pKa = 6.05LLAMVAVGIIVLFIVPP61 pKa = 4.41

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

23264

39

3403

205.9

23.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.401 ± 0.531

0.993 ± 0.156

5.799 ± 0.129

7.058 ± 0.286

3.624 ± 0.133

6.676 ± 0.243

2.149 ± 0.207

4.862 ± 0.167

6.147 ± 0.287

9.237 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.146 ± 0.123

4.436 ± 0.169

3.783 ± 0.177

4.406 ± 0.26

5.266 ± 0.182

5.872 ± 0.199

5.536 ± 0.214

6.77 ± 0.162

1.367 ± 0.168

3.473 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski