Actinomadura sp. J1-007

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I4PQL0|A0A6I4PQL0_9ACTN Helix-turn-helix domain-containing protein OS=Actinomadura sp. J1-007 OX=2661913 GN=GEV43_24405 PE=4 SV=1
MM1 pKa = 6.15TTYY4 pKa = 10.39IVVFGLIVVVVLVVVLVALGMRR26 pKa = 11.84ASRR29 pKa = 11.84AGQDD33 pKa = 3.48DD34 pKa = 5.38DD35 pKa = 5.87DD36 pKa = 5.3WDD38 pKa = 4.68DD39 pKa = 3.73QPQPQPRR46 pKa = 11.84GRR48 pKa = 11.84RR49 pKa = 11.84GVPAEE54 pKa = 5.28DD55 pKa = 3.54MGQDD59 pKa = 3.53AMGGGYY65 pKa = 10.6DD66 pKa = 3.68NGYY69 pKa = 10.94DD70 pKa = 3.5EE71 pKa = 5.88AGYY74 pKa = 10.31DD75 pKa = 3.28AAGYY79 pKa = 10.44DD80 pKa = 3.82GAQYY84 pKa = 11.13GPGSADD90 pKa = 2.84PGYY93 pKa = 10.65DD94 pKa = 2.67RR95 pKa = 11.84RR96 pKa = 11.84VAGAPGGGQGGPGGPQGGGPLAAPAAAPAPPPPAPAGPASDD137 pKa = 4.14EE138 pKa = 4.48MEE140 pKa = 5.67DD141 pKa = 3.45DD142 pKa = 5.39DD143 pKa = 4.41YY144 pKa = 11.55WATITFDD151 pKa = 3.31KK152 pKa = 11.02PKK154 pKa = 10.48FPWQHH159 pKa = 6.61DD160 pKa = 4.04GQPEE164 pKa = 3.87RR165 pKa = 11.84SEE167 pKa = 5.02AEE169 pKa = 4.02MAADD173 pKa = 4.2PLNNPQAPAAEE184 pKa = 4.26QHH186 pKa = 6.36APPAAEE192 pKa = 4.85PPNLTQPVPMGADD205 pKa = 3.38LHH207 pKa = 6.88ALGGVSGPPPAPQGPGGPGGPGGMGTGPQPSGTNPFPGGSPFGGDD252 pKa = 3.4PSATANDD259 pKa = 5.98GIPADD264 pKa = 3.92IGGHH268 pKa = 4.92GQQGPYY274 pKa = 10.68GLGGPGGPDD283 pKa = 2.93QPVYY287 pKa = 10.84GHH289 pKa = 6.09EE290 pKa = 4.02QQPSYY295 pKa = 10.74GAPDD299 pKa = 3.62PSGYY303 pKa = 10.57GGQDD307 pKa = 3.03PSGYY311 pKa = 10.12GQEE314 pKa = 4.09QPSYY318 pKa = 10.72GGDD321 pKa = 3.31PSSPFGVPEE330 pKa = 3.74QQPAYY335 pKa = 10.43GSAQDD340 pKa = 3.45QPSYY344 pKa = 11.27GGAQDD349 pKa = 3.37QPAYY353 pKa = 10.18GGQPEE358 pKa = 4.22QPGPYY363 pKa = 9.91GGGQDD368 pKa = 3.36QPSYY372 pKa = 11.26GDD374 pKa = 3.71YY375 pKa = 11.26GSDD378 pKa = 3.53PLGSRR383 pKa = 11.84PAAAASPPPPPQGRR397 pKa = 11.84PDD399 pKa = 3.78AFDD402 pKa = 4.0MPLSPPAPNAPDD414 pKa = 3.77PRR416 pKa = 11.84ASDD419 pKa = 3.87PLGLPLGRR427 pKa = 11.84TEE429 pKa = 4.89EE430 pKa = 4.3PAPPPAPPAPRR441 pKa = 11.84PAGSDD446 pKa = 3.23TDD448 pKa = 3.61NHH450 pKa = 6.45KK451 pKa = 11.04LPTVDD456 pKa = 5.16EE457 pKa = 4.24LLQRR461 pKa = 11.84IQTDD465 pKa = 3.71RR466 pKa = 11.84QRR468 pKa = 11.84TSGPSDD474 pKa = 2.91QGGSYY479 pKa = 10.87GGGSLNDD486 pKa = 4.02PLGDD490 pKa = 3.79PLGSGSFGTGGGAGIGGSSTGPWAPPPSSGPPSGQGGYY528 pKa = 10.62DD529 pKa = 3.48SDD531 pKa = 4.44PAGSTMSYY539 pKa = 10.85GGGLPAGGSGYY550 pKa = 10.9GQGSQNDD557 pKa = 5.15GYY559 pKa = 9.53PASPAYY565 pKa = 10.12GDD567 pKa = 3.39SSRR570 pKa = 11.84YY571 pKa = 9.75DD572 pKa = 3.48DD573 pKa = 4.47PLGGSGRR580 pKa = 11.84DD581 pKa = 3.39SYY583 pKa = 12.1GSGQGGGMSPAPTGEE598 pKa = 3.86PGRR601 pKa = 11.84YY602 pKa = 9.29GDD604 pKa = 4.93FSGSSYY610 pKa = 11.42NGADD614 pKa = 3.9PLSAPSDD621 pKa = 3.68PGGSGGNYY629 pKa = 9.86DD630 pKa = 3.96PNATQAYY637 pKa = 8.55GSGYY641 pKa = 9.79MNQQSGGYY649 pKa = 7.48QAPSGGDD656 pKa = 3.12TGPYY660 pKa = 8.77GSSYY664 pKa = 10.05PGSDD668 pKa = 3.27QRR670 pKa = 11.84QPDD673 pKa = 3.36DD674 pKa = 3.64WEE676 pKa = 3.72NHH678 pKa = 4.52RR679 pKa = 11.84DD680 pKa = 3.59YY681 pKa = 11.29RR682 pKa = 11.84RR683 pKa = 3.16

Molecular weight: 67.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I4PMR3|A0A6I4PMR3_9ACTN D-alanine--D-alanine ligase OS=Actinomadura sp. J1-007 OX=2661913 GN=ddl PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSGRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ARR42 pKa = 11.84IAVV45 pKa = 3.5

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8101

0

8101

2547833

29

4770

314.5

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.301 ± 0.045

0.793 ± 0.007

5.912 ± 0.019

5.47 ± 0.025

2.745 ± 0.014

9.99 ± 0.027

2.135 ± 0.012

3.078 ± 0.016

1.873 ± 0.022

10.175 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.783 ± 0.01

1.673 ± 0.013

6.465 ± 0.026

2.307 ± 0.014

9.014 ± 0.034

4.818 ± 0.021

5.5 ± 0.023

8.469 ± 0.025

1.512 ± 0.011

1.987 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski