Pseudomonas phage phi13

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus; Pseudomonas virus phi13

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9FZT6|Q9FZT6_9VIRU p3a OS=Pseudomonas phage phi13 OX=134554 GN=3a PE=4 SV=1
MM1 pKa = 8.35DD2 pKa = 6.43DD3 pKa = 4.87NNLLDD8 pKa = 3.77IPVDD12 pKa = 3.54HH13 pKa = 6.63VFTRR17 pKa = 11.84TEE19 pKa = 4.02VAEE22 pKa = 4.31GTAAGFAASGLLAVLPEE39 pKa = 4.18LKK41 pKa = 9.16KK42 pKa = 8.83TQLTEE47 pKa = 3.69ILSVSEE53 pKa = 4.37FDD55 pKa = 3.74GTSDD59 pKa = 3.79LMDD62 pKa = 3.33VVVSPNVVTRR72 pKa = 11.84VWLFQAISEE81 pKa = 4.44YY82 pKa = 10.13IARR85 pKa = 11.84GDD87 pKa = 3.57ADD89 pKa = 3.53GHH91 pKa = 4.74VV92 pKa = 3.59

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9FZU1|Q9FZU1_9VIRU p12 OS=Pseudomonas phage phi13 OX=134554 GN=12 PE=4 SV=1
MM1 pKa = 7.53GLYY4 pKa = 7.15TTYY7 pKa = 10.71KK8 pKa = 10.13KK9 pKa = 10.64SAEE12 pKa = 3.95QTVDD16 pKa = 3.58KK17 pKa = 10.94EE18 pKa = 3.93NDD20 pKa = 3.74SIGKK24 pKa = 9.44KK25 pKa = 9.64VVGFLTAPVSFKK37 pKa = 11.29DD38 pKa = 4.12DD39 pKa = 3.54VLSLRR44 pKa = 11.84NPSHH48 pKa = 5.17QRR50 pKa = 11.84SRR52 pKa = 11.84LGRR55 pKa = 11.84SDD57 pKa = 3.52LPGRR61 pKa = 11.84RR62 pKa = 11.84VAGSPPCGG70 pKa = 3.23

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

3746

38

801

288.2

31.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.559 ± 0.637

0.507 ± 0.151

5.713 ± 0.556

4.778 ± 0.412

3.764 ± 0.327

7.341 ± 0.38

1.789 ± 0.277

4.939 ± 0.397

3.951 ± 0.404

9.824 ± 0.491

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.097 ± 0.315

3.284 ± 0.409

5.099 ± 0.442

2.776 ± 0.343

5.446 ± 0.55

8.089 ± 0.468

5.873 ± 0.312

8.142 ± 0.607

1.575 ± 0.324

2.456 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski